Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.

Apoptosis Inducing Factor (AIF) is a highly conserved, ubiquitous flavoprotein localized in the mitochondrial intermembrane space. In vivo, AIF provides protection against neuronal and cardiomyocyte apoptosis induced by oxidative stress. Conversely in vitro, AIF has been demonstrated to have a pro-a...

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Autores principales:	Anne-Sophie Armand, Iman Laziz, Dounia Djeghloul, Sylvie Lécolle, Anne T Bertrand, Olivier Biondi, Leon J De Windt, Christophe Chanoine
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd65edbb7d954aa8a79f6e7af60092b8
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spelling	oai:doaj.org-article:cd65edbb7d954aa8a79f6e7af60092b82021-11-18T07:34:54ZApoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.1932-620310.1371/journal.pone.0027283https://doaj.org/article/cd65edbb7d954aa8a79f6e7af60092b82011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22076146/?tool=EBIhttps://doaj.org/toc/1932-6203Apoptosis Inducing Factor (AIF) is a highly conserved, ubiquitous flavoprotein localized in the mitochondrial intermembrane space. In vivo, AIF provides protection against neuronal and cardiomyocyte apoptosis induced by oxidative stress. Conversely in vitro, AIF has been demonstrated to have a pro-apoptotic role upon induction of the mitochondrial death pathway, once AIF translocates to the nucleus where it facilitates chromatin condensation and large scale DNA fragmentation. Given that the aif hypomorphic harlequin (Hq) mutant mouse model displays severe sarcopenia, we examined skeletal muscle from the aif hypomorphic mice in more detail. Adult AIF-deficient skeletal myofibers display oxidative stress and a severe form of atrophy, associated with a loss of myonuclei and a fast to slow fiber type switch, both in "slow" muscles such as soleus, as well as in "fast" muscles such as extensor digitorum longus, most likely resulting from an increase of MEF2 activity. This fiber type switch was conserved in regenerated soleus and EDL muscles of Hq mice subjected to cardiotoxin injection. In addition, muscle regeneration in soleus and EDL muscles of Hq mice was severely delayed. Freshly cultured myofibers, soleus and EDL muscle sections from Hq mice displayed a decreased satellite cell pool, which could be rescued by pretreating aif hypomorphic mice with the manganese-salen free radical scavenger EUK-8. Satellite cell activation seems to be abnormally long in Hq primary culture compared to controls. However, AIF deficiency did not affect myoblast cell proliferation and differentiation. Thus, AIF protects skeletal muscles against oxidative stress-induced damage probably by protecting satellite cells against oxidative stress and maintaining skeletal muscle stem cell number and activation.Anne-Sophie ArmandIman LazizDounia DjeghloulSylvie LécolleAnne T BertrandOlivier BiondiLeon J De WindtChristophe ChanoinePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27283 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anne-Sophie Armand Iman Laziz Dounia Djeghloul Sylvie Lécolle Anne T Bertrand Olivier Biondi Leon J De Windt Christophe Chanoine Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.
description	Apoptosis Inducing Factor (AIF) is a highly conserved, ubiquitous flavoprotein localized in the mitochondrial intermembrane space. In vivo, AIF provides protection against neuronal and cardiomyocyte apoptosis induced by oxidative stress. Conversely in vitro, AIF has been demonstrated to have a pro-apoptotic role upon induction of the mitochondrial death pathway, once AIF translocates to the nucleus where it facilitates chromatin condensation and large scale DNA fragmentation. Given that the aif hypomorphic harlequin (Hq) mutant mouse model displays severe sarcopenia, we examined skeletal muscle from the aif hypomorphic mice in more detail. Adult AIF-deficient skeletal myofibers display oxidative stress and a severe form of atrophy, associated with a loss of myonuclei and a fast to slow fiber type switch, both in "slow" muscles such as soleus, as well as in "fast" muscles such as extensor digitorum longus, most likely resulting from an increase of MEF2 activity. This fiber type switch was conserved in regenerated soleus and EDL muscles of Hq mice subjected to cardiotoxin injection. In addition, muscle regeneration in soleus and EDL muscles of Hq mice was severely delayed. Freshly cultured myofibers, soleus and EDL muscle sections from Hq mice displayed a decreased satellite cell pool, which could be rescued by pretreating aif hypomorphic mice with the manganese-salen free radical scavenger EUK-8. Satellite cell activation seems to be abnormally long in Hq primary culture compared to controls. However, AIF deficiency did not affect myoblast cell proliferation and differentiation. Thus, AIF protects skeletal muscles against oxidative stress-induced damage probably by protecting satellite cells against oxidative stress and maintaining skeletal muscle stem cell number and activation.
format	article
author	Anne-Sophie Armand Iman Laziz Dounia Djeghloul Sylvie Lécolle Anne T Bertrand Olivier Biondi Leon J De Windt Christophe Chanoine
author_facet	Anne-Sophie Armand Iman Laziz Dounia Djeghloul Sylvie Lécolle Anne T Bertrand Olivier Biondi Leon J De Windt Christophe Chanoine
author_sort	Anne-Sophie Armand
title	Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.
title_short	Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.
title_full	Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.
title_fullStr	Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.
title_full_unstemmed	Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.
title_sort	apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/cd65edbb7d954aa8a79f6e7af60092b8
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Apoptosis-inducing factor regulates skeletal muscle progenitor cell number and muscle phenotype.

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