Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria

Abstract. Ayuningrum D, Kristiana R, Nisa AA, Radjasa SK, Muchlissin SI, Radjasa OK, Sabdono A, Trianto A. 2019. Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria. Biodiversitas 20: 956-964. Tunicate is a rich secondar...

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Autores principales:	DIAH AYUNINGRUM, RHESI KRISTIANA, AYUNDA AINUN NISA, SEPTHY KUSUMA RADJASA, SAKTI IMAM MUCHLISSIN, OCKY KARNA RADJASA, AGUS SABDONO, AGUS TRIANTO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cd6811e5936b41b1a9e002ba15b153a6
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spelling	oai:doaj.org-article:cd6811e5936b41b1a9e002ba15b153a62021-11-21T21:40:39ZBacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria1412-033X2085-472210.13057/biodiv/d200404https://doaj.org/article/cd6811e5936b41b1a9e002ba15b153a62019-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3510https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ayuningrum D, Kristiana R, Nisa AA, Radjasa SK, Muchlissin SI, Radjasa OK, Sabdono A, Trianto A. 2019. Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria. Biodiversitas 20: 956-964. Tunicate is a rich secondary metabolites producer with various biological activities whether as an original producer or produced by the associated microorganisms. In this study, a total of 11 tunicate specimens were identified as Polycarpa aurata with four color variations based on morphological characteristic and COI gene identification and BLAST analysis. The P. aurata associated-bacteria were isolated and tested for antimicrobial activity against multi-drug resistant (MDR) bacteria. A total of 86 axenic isolates were successfully purified. Furthermore, nine isolates (10.5%) exhibited antibacterial activity on preliminary screening. Nine prospective isolates were fermented in respective medium (Zobell 2216, modified M1 or modified ISP2 media) then extracted using ethyl acetate. The ethyl acetate extracts from liquid fermentation were tested against MDR Escherichia coli, MDR Bacillus cereus, Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Sensitive and Staphylococcus aureus (MSSA). As a result, seven isolates (8.1%) still retained the activity at the extract concentration 150 µg/disk. Molecular analysis based on 16S rDNA sequencing revealed the most active isolates, TSB 47, TSC 10 and TSB 34 identified as Bacillus tropicus, Vibrio alginolyticus and Virgibacillus massiliensis, with BLAST homology 99%.DIAH AYUNINGRUMRHESI KRISTIANAAYUNDA AINUN NISASEPTHY KUSUMA RADJASASAKTI IMAM MUCHLISSINOCKY KARNA RADJASAAGUS SABDONOAGUS TRIANTOMBI & UNS SoloarticleBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 4, Pp 956-964 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 DIAH AYUNINGRUM RHESI KRISTIANA AYUNDA AINUN NISA SEPTHY KUSUMA RADJASA SAKTI IMAM MUCHLISSIN OCKY KARNA RADJASA AGUS SABDONO AGUS TRIANTO Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria
description	Abstract. Ayuningrum D, Kristiana R, Nisa AA, Radjasa SK, Muchlissin SI, Radjasa OK, Sabdono A, Trianto A. 2019. Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria. Biodiversitas 20: 956-964. Tunicate is a rich secondary metabolites producer with various biological activities whether as an original producer or produced by the associated microorganisms. In this study, a total of 11 tunicate specimens were identified as Polycarpa aurata with four color variations based on morphological characteristic and COI gene identification and BLAST analysis. The P. aurata associated-bacteria were isolated and tested for antimicrobial activity against multi-drug resistant (MDR) bacteria. A total of 86 axenic isolates were successfully purified. Furthermore, nine isolates (10.5%) exhibited antibacterial activity on preliminary screening. Nine prospective isolates were fermented in respective medium (Zobell 2216, modified M1 or modified ISP2 media) then extracted using ethyl acetate. The ethyl acetate extracts from liquid fermentation were tested against MDR Escherichia coli, MDR Bacillus cereus, Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Sensitive and Staphylococcus aureus (MSSA). As a result, seven isolates (8.1%) still retained the activity at the extract concentration 150 µg/disk. Molecular analysis based on 16S rDNA sequencing revealed the most active isolates, TSB 47, TSC 10 and TSB 34 identified as Bacillus tropicus, Vibrio alginolyticus and Virgibacillus massiliensis, with BLAST homology 99%.
format	article
author	DIAH AYUNINGRUM RHESI KRISTIANA AYUNDA AINUN NISA SEPTHY KUSUMA RADJASA SAKTI IMAM MUCHLISSIN OCKY KARNA RADJASA AGUS SABDONO AGUS TRIANTO
author_facet	DIAH AYUNINGRUM RHESI KRISTIANA AYUNDA AINUN NISA SEPTHY KUSUMA RADJASA SAKTI IMAM MUCHLISSIN OCKY KARNA RADJASA AGUS SABDONO AGUS TRIANTO
author_sort	DIAH AYUNINGRUM
title	Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria
title_short	Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria
title_full	Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria
title_fullStr	Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria
title_full_unstemmed	Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria
title_sort	bacteria associated with tunicate, polycarpa aurata, from lease sea, maluku, indonesia exhibiting anti-multidrug resistant bacteria
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/cd6811e5936b41b1a9e002ba15b153a6
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Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria

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