Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen

The current study was a part of the project on generating viral particle traps occurring due to covalent immobilization on the interface of recombinant virus-specific polymer-based nano- and microparticles. It is assumed that protein-particle conjugates could be able to bind virions followed by engu...

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Autores principales:	R. G. Sakhabeev, D. S. Polyakov, A. D. Goshina, A. A. Vishnya, I. V. Kudryavtsev, E. S. Sinitcina, V. А. Korzhikov-Vlakh, T. B. Tennikova, M. M. Shavlovsky
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	pla-based microparticles pla-peg-based nanoparticles virus “traps” green fluorescent protein t-cell immune response Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/cd767eb94c63444c817009717ac208ad
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spelling	oai:doaj.org-article:cd767eb94c63444c817009717ac208ad2021-11-22T07:09:55ZEnhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen2220-76192313-739810.15789/2220-7619-ETS-1374https://doaj.org/article/cd767eb94c63444c817009717ac208ad2021-09-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1374https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The current study was a part of the project on generating viral particle traps occurring due to covalent immobilization on the interface of recombinant virus-specific polymer-based nano- and microparticles. It is assumed that protein-particle conjugates could be able to bind virions followed by engulfment by immune cells. The study was aimed to examine the effect of polylactic acid (PLA) and PLA block-copolymer with polyethylene glycol (PLA-PEG)-based micro- and nanoparticles on the cellular immune response against polymeric particle-bound antigen. Materials and methods. A recombinant chimeric protein beta-2-microglobulin — green fluorescent protein (β2M-sfGFP) was obtained by affine chromatography. The recombinant protein was immobilized onto the polymer particles, which were further used for mice immunization. Female F1 hybrid mice (CBA x C57BL) in experimental and control groups consisted of 4–6-month-old 15 animals (weighted 20–25 g). Intracellular cytokine staining was used to evaluate the cellular immune response. Results and discussion. It was shown that the nanoparticles of PLA block-copolymer with polyethylene glycol (PLA-PEG) were able to bind 10 microgram protein per 1 mg polymer. The polylactic acid nanoparticles were able to bind 2,3 microgram protein per 1 mg polymer. In experiment, mice in group 1 were immunized with 100 nm PLA-PEG particle-β2M-sfGFP conjugate, in group 2 — with same particles together with soluble β2M-sfGFP. In group 3, mice were immunized with 1400 nm PLA particles-β2M-sfGFP conjugate, and in group 4 — with same particles together with soluble protein. The spleens isolated 2 weeks after the four-time intraperitoneal immunization. Comparison of immune response between groups was assessed by nonparametric Kruskal–Wallis criterion with Tukey correction. It was shown that the number of antigen-specific CD4+ T cells produced to model protein was significantly higher after immunization with particle-β2M-sfGFP conjugate, as compared to control groups, wherein immunization was performed with a mixture of protein and unmodified particles (p < 0.001). It was found that the number of antigen-specific CD8+ T cells formed against β2m-sfGFP did not differ between all groups examined.R. G. SakhabeevD. S. PolyakovA. D. GoshinaA. A. VishnyaI. V. KudryavtsevE. S. SinitcinaV. А. Korzhikov-VlakhT. B. TennikovaM. M. ShavlovskySankt-Peterburg : NIIÈM imeni Pasteraarticlepla-based microparticlespla-peg-based nanoparticlesvirus “traps”green fluorescent proteint-cell immune responseInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 4, Pp 777-783 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	pla-based microparticles pla-peg-based nanoparticles virus “traps” green fluorescent protein t-cell immune response Infectious and parasitic diseases RC109-216
spellingShingle	pla-based microparticles pla-peg-based nanoparticles virus “traps” green fluorescent protein t-cell immune response Infectious and parasitic diseases RC109-216 R. G. Sakhabeev D. S. Polyakov A. D. Goshina A. A. Vishnya I. V. Kudryavtsev E. S. Sinitcina V. А. Korzhikov-Vlakh T. B. Tennikova M. M. Shavlovsky Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen
description	The current study was a part of the project on generating viral particle traps occurring due to covalent immobilization on the interface of recombinant virus-specific polymer-based nano- and microparticles. It is assumed that protein-particle conjugates could be able to bind virions followed by engulfment by immune cells. The study was aimed to examine the effect of polylactic acid (PLA) and PLA block-copolymer with polyethylene glycol (PLA-PEG)-based micro- and nanoparticles on the cellular immune response against polymeric particle-bound antigen. Materials and methods. A recombinant chimeric protein beta-2-microglobulin — green fluorescent protein (β2M-sfGFP) was obtained by affine chromatography. The recombinant protein was immobilized onto the polymer particles, which were further used for mice immunization. Female F1 hybrid mice (CBA x C57BL) in experimental and control groups consisted of 4–6-month-old 15 animals (weighted 20–25 g). Intracellular cytokine staining was used to evaluate the cellular immune response. Results and discussion. It was shown that the nanoparticles of PLA block-copolymer with polyethylene glycol (PLA-PEG) were able to bind 10 microgram protein per 1 mg polymer. The polylactic acid nanoparticles were able to bind 2,3 microgram protein per 1 mg polymer. In experiment, mice in group 1 were immunized with 100 nm PLA-PEG particle-β2M-sfGFP conjugate, in group 2 — with same particles together with soluble β2M-sfGFP. In group 3, mice were immunized with 1400 nm PLA particles-β2M-sfGFP conjugate, and in group 4 — with same particles together with soluble protein. The spleens isolated 2 weeks after the four-time intraperitoneal immunization. Comparison of immune response between groups was assessed by nonparametric Kruskal–Wallis criterion with Tukey correction. It was shown that the number of antigen-specific CD4+ T cells produced to model protein was significantly higher after immunization with particle-β2M-sfGFP conjugate, as compared to control groups, wherein immunization was performed with a mixture of protein and unmodified particles (p < 0.001). It was found that the number of antigen-specific CD8+ T cells formed against β2m-sfGFP did not differ between all groups examined.
format	article
author	R. G. Sakhabeev D. S. Polyakov A. D. Goshina A. A. Vishnya I. V. Kudryavtsev E. S. Sinitcina V. А. Korzhikov-Vlakh T. B. Tennikova M. M. Shavlovsky
author_facet	R. G. Sakhabeev D. S. Polyakov A. D. Goshina A. A. Vishnya I. V. Kudryavtsev E. S. Sinitcina V. А. Korzhikov-Vlakh T. B. Tennikova M. M. Shavlovsky
author_sort	R. G. Sakhabeev
title	Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen
title_short	Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen
title_full	Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen
title_fullStr	Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen
title_full_unstemmed	Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen
title_sort	enhancing the specific t cell immune response against micro- and nanoparticle immobilized antigen
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/cd767eb94c63444c817009717ac208ad
work_keys_str_mv	AT rgsakhabeev enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen AT dspolyakov enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen AT adgoshina enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen AT aavishnya enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen AT ivkudryavtsev enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen AT essinitcina enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen AT vakorzhikovvlakh enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen AT tbtennikova enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen AT mmshavlovsky enhancingthespecifictcellimmuneresponseagainstmicroandnanoparticleimmobilizedantigen
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Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen

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