MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.

Metabolic interaction via lactate between glial cells and neurons has been proposed as one of the mechanisms involved in hypothalamic glucosensing. We have postulated that hypothalamic glial cells, also known as tanycytes, produce lactate by glycolytic metabolism of glucose. Transfer of lactate to n...

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Autores principales:	Christian Cortés-Campos, Roberto Elizondo, Paula Llanos, Romina María Uranga, Francisco Nualart, María Angeles García
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd8ee6c04b214617b5d61c39d9ecbf4e
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spelling	oai:doaj.org-article:cd8ee6c04b214617b5d61c39d9ecbf4e2021-11-18T06:59:38ZMCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.1932-620310.1371/journal.pone.0016411https://doaj.org/article/cd8ee6c04b214617b5d61c39d9ecbf4e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21297988/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Metabolic interaction via lactate between glial cells and neurons has been proposed as one of the mechanisms involved in hypothalamic glucosensing. We have postulated that hypothalamic glial cells, also known as tanycytes, produce lactate by glycolytic metabolism of glucose. Transfer of lactate to neighboring neurons stimulates ATP synthesis and thus contributes to their activation. Because destruction of third ventricle (III-V) tanycytes is sufficient to alter blood glucose levels and food intake in rats, it is hypothesized that tanycytes are involved in the hypothalamic glucose sensing mechanism. Here, we demonstrate the presence and function of monocarboxylate transporters (MCTs) in tanycytes. Specifically, MCT1 and MCT4 expression as well as their distribution were analyzed in Sprague Dawley rat brain, and we demonstrate that both transporters are expressed in tanycytes. Using primary tanycyte cultures, kinetic analyses and sensitivity to inhibitors were undertaken to confirm that MCT1 and MCT4 were functional for lactate influx. Additionally, physiological concentrations of glucose induced lactate efflux in cultured tanycytes, which was inhibited by classical MCT inhibitors. Because the expression of both MCT1 and MCT4 has been linked to lactate efflux, we propose that tanycytes participate in glucose sensing based on a metabolic interaction with neurons of the arcuate nucleus, which are stimulated by lactate released from MCT1 and MCT4-expressing tanycytes.Christian Cortés-CamposRoberto ElizondoPaula LlanosRomina María UrangaFrancisco NualartMaría Angeles GarcíaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16411 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Cortés-Campos Roberto Elizondo Paula Llanos Romina María Uranga Francisco Nualart María Angeles García MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.
description	Metabolic interaction via lactate between glial cells and neurons has been proposed as one of the mechanisms involved in hypothalamic glucosensing. We have postulated that hypothalamic glial cells, also known as tanycytes, produce lactate by glycolytic metabolism of glucose. Transfer of lactate to neighboring neurons stimulates ATP synthesis and thus contributes to their activation. Because destruction of third ventricle (III-V) tanycytes is sufficient to alter blood glucose levels and food intake in rats, it is hypothesized that tanycytes are involved in the hypothalamic glucose sensing mechanism. Here, we demonstrate the presence and function of monocarboxylate transporters (MCTs) in tanycytes. Specifically, MCT1 and MCT4 expression as well as their distribution were analyzed in Sprague Dawley rat brain, and we demonstrate that both transporters are expressed in tanycytes. Using primary tanycyte cultures, kinetic analyses and sensitivity to inhibitors were undertaken to confirm that MCT1 and MCT4 were functional for lactate influx. Additionally, physiological concentrations of glucose induced lactate efflux in cultured tanycytes, which was inhibited by classical MCT inhibitors. Because the expression of both MCT1 and MCT4 has been linked to lactate efflux, we propose that tanycytes participate in glucose sensing based on a metabolic interaction with neurons of the arcuate nucleus, which are stimulated by lactate released from MCT1 and MCT4-expressing tanycytes.
format	article
author	Christian Cortés-Campos Roberto Elizondo Paula Llanos Romina María Uranga Francisco Nualart María Angeles García
author_facet	Christian Cortés-Campos Roberto Elizondo Paula Llanos Romina María Uranga Francisco Nualart María Angeles García
author_sort	Christian Cortés-Campos
title	MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.
title_short	MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.
title_full	MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.
title_fullStr	MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.
title_full_unstemmed	MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.
title_sort	mct expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/cd8ee6c04b214617b5d61c39d9ecbf4e
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MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction.

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