Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.

The transcription factor etsrp/Er71/Etv2 is a master control gene for vasculogenesis in all species studied to date. It is also required for hematopoiesis in zebrafish and mice. Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos o...

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Autores principales:	Gustavo Gomez, Jae-Hyung Lee, Matthew B Veldman, Jing Lu, Xinshu Xiao, Shuo Lin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd93bc4cac0a4717bfae0e9a0b64977e
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spelling	oai:doaj.org-article:cd93bc4cac0a4717bfae0e9a0b64977e2021-11-18T07:25:03ZIdentification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.1932-620310.1371/journal.pone.0031658https://doaj.org/article/cd93bc4cac0a4717bfae0e9a0b64977e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22438865/?tool=EBIhttps://doaj.org/toc/1932-6203The transcription factor etsrp/Er71/Etv2 is a master control gene for vasculogenesis in all species studied to date. It is also required for hematopoiesis in zebrafish and mice. Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos overexpressing etsrp by microarrays. Here we re-examined this transcriptional profile by Illumina RNA-sequencing technology, revealing a substantially increased number of candidate genes regulated by etsrp. Expression studies of 50 selected candidate genes from this dataset resulted in the identification of 39 new genes that are expressed in vascular cells. Regulation of these genes by etsrp was confirmed by their ectopic induction in etsrp overexpressing and decreased expression in etsrp deficient embryos. Our studies demonstrate the effectiveness of the RNA-sequencing technology to identify biologically relevant genes in zebrfish and produced a comprehensive profile of genes previously unexplored in vascular endothelial cell biology.Gustavo GomezJae-Hyung LeeMatthew B VeldmanJing LuXinshu XiaoShuo LinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e31658 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gustavo Gomez Jae-Hyung Lee Matthew B Veldman Jing Lu Xinshu Xiao Shuo Lin Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
description	The transcription factor etsrp/Er71/Etv2 is a master control gene for vasculogenesis in all species studied to date. It is also required for hematopoiesis in zebrafish and mice. Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos overexpressing etsrp by microarrays. Here we re-examined this transcriptional profile by Illumina RNA-sequencing technology, revealing a substantially increased number of candidate genes regulated by etsrp. Expression studies of 50 selected candidate genes from this dataset resulted in the identification of 39 new genes that are expressed in vascular cells. Regulation of these genes by etsrp was confirmed by their ectopic induction in etsrp overexpressing and decreased expression in etsrp deficient embryos. Our studies demonstrate the effectiveness of the RNA-sequencing technology to identify biologically relevant genes in zebrfish and produced a comprehensive profile of genes previously unexplored in vascular endothelial cell biology.
format	article
author	Gustavo Gomez Jae-Hyung Lee Matthew B Veldman Jing Lu Xinshu Xiao Shuo Lin
author_facet	Gustavo Gomez Jae-Hyung Lee Matthew B Veldman Jing Lu Xinshu Xiao Shuo Lin
author_sort	Gustavo Gomez
title	Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
title_short	Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
title_full	Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
title_fullStr	Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
title_full_unstemmed	Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
title_sort	identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/cd93bc4cac0a4717bfae0e9a0b64977e
work_keys_str_mv	AT gustavogomez identificationofvascularandhematopoieticgenesdownstreamofetsrpbydeepsequencinginzebrafish AT jaehyunglee identificationofvascularandhematopoieticgenesdownstreamofetsrpbydeepsequencinginzebrafish AT matthewbveldman identificationofvascularandhematopoieticgenesdownstreamofetsrpbydeepsequencinginzebrafish AT jinglu identificationofvascularandhematopoieticgenesdownstreamofetsrpbydeepsequencinginzebrafish AT xinshuxiao identificationofvascularandhematopoieticgenesdownstreamofetsrpbydeepsequencinginzebrafish AT shuolin identificationofvascularandhematopoieticgenesdownstreamofetsrpbydeepsequencinginzebrafish
_version_	1718423500533792768

Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.

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