Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape

Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.

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Detalles Bibliográficos
Autores principales: Boyang Hua, Christopher P. Jones, Jaba Mitra, Peter J. Murray, Rebecca Rosenthal, Adrian R. Ferré-D’Amaré, Taekjip Ha
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/cd9a444440f54f6dbaa2b5c8d83e460c
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Descripción
Sumario:Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.