Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape

Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.

Guardado en:
Detalles Bibliográficos
Autores principales: Boyang Hua, Christopher P. Jones, Jaba Mitra, Peter J. Murray, Rebecca Rosenthal, Adrian R. Ferré-D’Amaré, Taekjip Ha
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
Q
Acceso en línea:https://doaj.org/article/cd9a444440f54f6dbaa2b5c8d83e460c
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:cd9a444440f54f6dbaa2b5c8d83e460c
record_format dspace
spelling oai:doaj.org-article:cd9a444440f54f6dbaa2b5c8d83e460c2021-12-02T14:55:15ZReal-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape10.1038/s41467-020-18283-12041-1723https://doaj.org/article/cd9a444440f54f6dbaa2b5c8d83e460c2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18283-1https://doaj.org/toc/2041-1723Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.Boyang HuaChristopher P. JonesJaba MitraPeter J. MurrayRebecca RosenthalAdrian R. Ferré-D’AmaréTaekjip HaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Boyang Hua
Christopher P. Jones
Jaba Mitra
Peter J. Murray
Rebecca Rosenthal
Adrian R. Ferré-D’Amaré
Taekjip Ha
Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
description Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.
format article
author Boyang Hua
Christopher P. Jones
Jaba Mitra
Peter J. Murray
Rebecca Rosenthal
Adrian R. Ferré-D’Amaré
Taekjip Ha
author_facet Boyang Hua
Christopher P. Jones
Jaba Mitra
Peter J. Murray
Rebecca Rosenthal
Adrian R. Ferré-D’Amaré
Taekjip Ha
author_sort Boyang Hua
title Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_short Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_full Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_fullStr Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_full_unstemmed Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_sort real-time monitoring of single ztp riboswitches reveals a complex and kinetically controlled decision landscape
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/cd9a444440f54f6dbaa2b5c8d83e460c
work_keys_str_mv AT boyanghua realtimemonitoringofsingleztpriboswitchesrevealsacomplexandkineticallycontrolleddecisionlandscape
AT christopherpjones realtimemonitoringofsingleztpriboswitchesrevealsacomplexandkineticallycontrolleddecisionlandscape
AT jabamitra realtimemonitoringofsingleztpriboswitchesrevealsacomplexandkineticallycontrolleddecisionlandscape
AT peterjmurray realtimemonitoringofsingleztpriboswitchesrevealsacomplexandkineticallycontrolleddecisionlandscape
AT rebeccarosenthal realtimemonitoringofsingleztpriboswitchesrevealsacomplexandkineticallycontrolleddecisionlandscape
AT adrianrferredamare realtimemonitoringofsingleztpriboswitchesrevealsacomplexandkineticallycontrolleddecisionlandscape
AT taekjipha realtimemonitoringofsingleztpriboswitchesrevealsacomplexandkineticallycontrolleddecisionlandscape
_version_ 1718389351713341440