Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape

Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.

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Main Authors:	Boyang Hua, Christopher P. Jones, Jaba Mitra, Peter J. Murray, Rebecca Rosenthal, Adrian R. Ferré-D’Amaré, Taekjip Ha
Format:	article
Language:	EN
Published:	Nature Portfolio 2020
Subjects:	Science Q
Online Access:	https://doaj.org/article/cd9a444440f54f6dbaa2b5c8d83e460c
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spelling	oai:doaj.org-article:cd9a444440f54f6dbaa2b5c8d83e460c2021-12-02T14:55:15ZReal-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape10.1038/s41467-020-18283-12041-1723https://doaj.org/article/cd9a444440f54f6dbaa2b5c8d83e460c2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18283-1https://doaj.org/toc/2041-1723Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.Boyang HuaChristopher P. JonesJaba MitraPeter J. MurrayRebecca RosenthalAdrian R. Ferré-D’AmaréTaekjip HaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Boyang Hua Christopher P. Jones Jaba Mitra Peter J. Murray Rebecca Rosenthal Adrian R. Ferré-D’Amaré Taekjip Ha Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
description	Many RNAs become functional before their synthesis completes. Here the authors employ a single-molecule vectorial folding assay mimicking RNA transcription and show that the ZTP riboswitch is kinetically controlled and activated by slower unwinding and strategic pausing.
format	article
author	Boyang Hua Christopher P. Jones Jaba Mitra Peter J. Murray Rebecca Rosenthal Adrian R. Ferré-D’Amaré Taekjip Ha
author_facet	Boyang Hua Christopher P. Jones Jaba Mitra Peter J. Murray Rebecca Rosenthal Adrian R. Ferré-D’Amaré Taekjip Ha
author_sort	Boyang Hua
title	Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_short	Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_full	Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_fullStr	Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_full_unstemmed	Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape
title_sort	real-time monitoring of single ztp riboswitches reveals a complex and kinetically controlled decision landscape
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/cd9a444440f54f6dbaa2b5c8d83e460c
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Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape

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