Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles

Exosomes are extracellular vehicles (EVs) that encapsulate genomic and proteomic material from the cell of origin that can be used as biomarkers for non-invasive disease diagnostics in point of care settings. The efficient and accurate detection, quantification, and molecular profiling of exosomes a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Saif Mohammad Ishraq Bari, Faria Binte Hossain, Gergana G. Nestorova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sensors exosomes lab-on-a-chip Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/cda4c2f4cb4f4121959d298e9fe80bfc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cda4c2f4cb4f4121959d298e9fe80bfc
record_format	dspace
spelling	oai:doaj.org-article:cda4c2f4cb4f4121959d298e9fe80bfc2021-11-25T18:58:10ZAdvances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles10.3390/s212276451424-8220https://doaj.org/article/cda4c2f4cb4f4121959d298e9fe80bfc2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/22/7645https://doaj.org/toc/1424-8220Exosomes are extracellular vehicles (EVs) that encapsulate genomic and proteomic material from the cell of origin that can be used as biomarkers for non-invasive disease diagnostics in point of care settings. The efficient and accurate detection, quantification, and molecular profiling of exosomes are crucial for the accurate identification of disease biomarkers. Conventional isolation methods, while well-established, provide the co-purification of proteins and other types of EVs. Exosome purification, characterization, and OMICS analysis are performed separately, which increases the complexity, duration, and cost of the process. Due to these constraints, the point-of-care and personalized analysis of exosomes are limited in clinical settings. Lab-on-a-chip biosensing has enabled the integration of isolation and characterization processes in a single platform. The presented review discusses recent advancements in biosensing technology for the separation and detection of exosomes. Fluorescent, colorimetric, electrochemical, magnetic, and surface plasmon resonance technologies have been developed for the quantification of exosomes in biological fluids. Size-exclusion filtration, immunoaffinity, electroactive, and acoustic-fluid-based technologies were successfully applied for the on-chip isolation of exosomes. The advancement of biosensing technology for the detection of exosomes provides better sensitivity and a reduced signal-to-noise ratio. The key challenge for the integration of clinical settings remains the lack of capabilities for on-chip genomic and proteomic analysis.Saif Mohammad Ishraq BariFaria Binte HossainGergana G. NestorovaMDPI AGarticlesensorsexosomeslab-on-a-chipChemical technologyTP1-1185ENSensors, Vol 21, Iss 7645, p 7645 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sensors exosomes lab-on-a-chip Chemical technology TP1-1185
spellingShingle	sensors exosomes lab-on-a-chip Chemical technology TP1-1185 Saif Mohammad Ishraq Bari Faria Binte Hossain Gergana G. Nestorova Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles
description	Exosomes are extracellular vehicles (EVs) that encapsulate genomic and proteomic material from the cell of origin that can be used as biomarkers for non-invasive disease diagnostics in point of care settings. The efficient and accurate detection, quantification, and molecular profiling of exosomes are crucial for the accurate identification of disease biomarkers. Conventional isolation methods, while well-established, provide the co-purification of proteins and other types of EVs. Exosome purification, characterization, and OMICS analysis are performed separately, which increases the complexity, duration, and cost of the process. Due to these constraints, the point-of-care and personalized analysis of exosomes are limited in clinical settings. Lab-on-a-chip biosensing has enabled the integration of isolation and characterization processes in a single platform. The presented review discusses recent advancements in biosensing technology for the separation and detection of exosomes. Fluorescent, colorimetric, electrochemical, magnetic, and surface plasmon resonance technologies have been developed for the quantification of exosomes in biological fluids. Size-exclusion filtration, immunoaffinity, electroactive, and acoustic-fluid-based technologies were successfully applied for the on-chip isolation of exosomes. The advancement of biosensing technology for the detection of exosomes provides better sensitivity and a reduced signal-to-noise ratio. The key challenge for the integration of clinical settings remains the lack of capabilities for on-chip genomic and proteomic analysis.
format	article
author	Saif Mohammad Ishraq Bari Faria Binte Hossain Gergana G. Nestorova
author_facet	Saif Mohammad Ishraq Bari Faria Binte Hossain Gergana G. Nestorova
author_sort	Saif Mohammad Ishraq Bari
title	Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles
title_short	Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles
title_full	Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles
title_fullStr	Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles
title_full_unstemmed	Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles
title_sort	advances in biosensors technology for detection and characterization of extracellular vesicles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cda4c2f4cb4f4121959d298e9fe80bfc
work_keys_str_mv	AT saifmohammadishraqbari advancesinbiosensorstechnologyfordetectionandcharacterizationofextracellularvesicles AT fariabintehossain advancesinbiosensorstechnologyfordetectionandcharacterizationofextracellularvesicles AT gerganagnestorova advancesinbiosensorstechnologyfordetectionandcharacterizationofextracellularvesicles
_version_	1718410490985578496

Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles

Ejemplares similares