Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains

Potato virus Y (PVY) and <i>Phytophthora infestans</i> (Mont.) de Bary that causes potato late blight (LB), pose serious constraints to cultivated potatoes due to significant yield reduction, and phenotyping for resistance remains challenging. Breeding operations for vegetatively propaga...

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Autores principales:	Moctar Kante, Hannele Lindqvist-Kreuze, Leticia Portal, Maria David, Manuel Gastelo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	potato late blight PVY quality control KASP Agriculture S
Acceso en línea:	https://doaj.org/article/cdae1abf3d8e496698523a9a1948bbc5
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spelling	oai:doaj.org-article:cdae1abf3d8e496698523a9a1948bbc52021-11-25T16:11:20ZKompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains10.3390/agronomy111123152073-4395https://doaj.org/article/cdae1abf3d8e496698523a9a1948bbc52021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2315https://doaj.org/toc/2073-4395Potato virus Y (PVY) and <i>Phytophthora infestans</i> (Mont.) de Bary that causes potato late blight (LB), pose serious constraints to cultivated potatoes due to significant yield reduction, and phenotyping for resistance remains challenging. Breeding operations for vegetatively propagated crops can lead to genotype mislabeling that, in turn, reduces genetic gains. Low-density and low-cost molecular marker assessment for phenotype prediction and quality control is a viable option for breeding programs. Here, we report on the development of kompetitive allele specific PCR (KASP) markers for LB and PVY resistance, and for routine quality control assessment of different breeding populations. Two KASP markers for LB resistance and two for PVY <i>Ryadg</i> were validated with an estimated assay power that ranged between 0.65 and 0.88. The developed QC KASP markers demonstrated the capability of discriminating tetraploid calls in breeding materials, including full-sibs and half-sibs. Routine implementation of the developed markers in a breeding program would assist with better allocation of resources and enable precise characterization of breeding material, thereby leading to increased genetic gains.Moctar KanteHannele Lindqvist-KreuzeLeticia PortalMaria DavidManuel GasteloMDPI AGarticlepotatolate blightPVYquality controlKASPAgricultureSENAgronomy, Vol 11, Iss 2315, p 2315 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	potato late blight PVY quality control KASP Agriculture S
spellingShingle	potato late blight PVY quality control KASP Agriculture S Moctar Kante Hannele Lindqvist-Kreuze Leticia Portal Maria David Manuel Gastelo Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains
description	Potato virus Y (PVY) and <i>Phytophthora infestans</i> (Mont.) de Bary that causes potato late blight (LB), pose serious constraints to cultivated potatoes due to significant yield reduction, and phenotyping for resistance remains challenging. Breeding operations for vegetatively propagated crops can lead to genotype mislabeling that, in turn, reduces genetic gains. Low-density and low-cost molecular marker assessment for phenotype prediction and quality control is a viable option for breeding programs. Here, we report on the development of kompetitive allele specific PCR (KASP) markers for LB and PVY resistance, and for routine quality control assessment of different breeding populations. Two KASP markers for LB resistance and two for PVY <i>Ryadg</i> were validated with an estimated assay power that ranged between 0.65 and 0.88. The developed QC KASP markers demonstrated the capability of discriminating tetraploid calls in breeding materials, including full-sibs and half-sibs. Routine implementation of the developed markers in a breeding program would assist with better allocation of resources and enable precise characterization of breeding material, thereby leading to increased genetic gains.
format	article
author	Moctar Kante Hannele Lindqvist-Kreuze Leticia Portal Maria David Manuel Gastelo
author_facet	Moctar Kante Hannele Lindqvist-Kreuze Leticia Portal Maria David Manuel Gastelo
author_sort	Moctar Kante
title	Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains
title_short	Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains
title_full	Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains
title_fullStr	Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains
title_full_unstemmed	Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains
title_sort	kompetitive allele specific pcr (kasp) markers for potato: an effective tool for increased genetic gains
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cdae1abf3d8e496698523a9a1948bbc5
work_keys_str_mv	AT moctarkante kompetitiveallelespecificpcrkaspmarkersforpotatoaneffectivetoolforincreasedgeneticgains AT hannelelindqvistkreuze kompetitiveallelespecificpcrkaspmarkersforpotatoaneffectivetoolforincreasedgeneticgains AT leticiaportal kompetitiveallelespecificpcrkaspmarkersforpotatoaneffectivetoolforincreasedgeneticgains AT mariadavid kompetitiveallelespecificpcrkaspmarkersforpotatoaneffectivetoolforincreasedgeneticgains AT manuelgastelo kompetitiveallelespecificpcrkaspmarkersforpotatoaneffectivetoolforincreasedgeneticgains
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Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains

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