An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker
Armin Salek Maghsoudi,1,* Shokoufeh Hassani,1,2,* Milad Rezaei Akmal,3 Mohammad Reza Ganjali,3,4 Kayvan Mirnia,5 Parviz Norouzi,3,4 Mohammad Abdollahi1,2 1Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 2Toxicology and Diseases Grou...
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Dove Medical Press
2020
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oai:doaj.org-article:cdae864379d24aec847b1d1515e8d2752021-12-02T08:05:19ZAn Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker1178-2013https://doaj.org/article/cdae864379d24aec847b1d1515e8d2752020-03-01T00:00:00Zhttps://www.dovepress.com/an-electrochemical-aptasensor-platform-based-on-flower-like-gold-micro-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Armin Salek Maghsoudi,1,* Shokoufeh Hassani,1,2,* Milad Rezaei Akmal,3 Mohammad Reza Ganjali,3,4 Kayvan Mirnia,5 Parviz Norouzi,3,4 Mohammad Abdollahi1,2 1Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 2Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran; 3Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran; 4Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran; 5Department of Neonatology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran*These authors contributed equally to this workCorrespondence: Mohammad AbdollahiToxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran 1417614411, IranTel +98 21 64122319Email mohammad@tums.ac.irPurpose: In the present study, a highly sensitive and simple electrochemical (EC) aptasensor for the detection of serpin A12 as a novel biomarker of diabetes was developed on a platform where flower-like gold microstructures (FLGMs) are electrodeposited onto a disposable screen-printed carbon electrode. Meanwhile, serpin A12-specific thiolated aptamer was covalently immobilized on the FLGMs.Methods: The electrochemical activity of a fabricated aptasensor under various conditions were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Aptamer concentration, deposition time, self-assembly time, and incubation time were optimized for assay of serpin A12. The differential pulse voltammetry (DPV) was implemented for quantitative detection of serpin A12 in K3 [Fe (CN) 6]/K4 [Fe (CN) 6] solution (redox probe).Results: The label-free aptasensor revealed a linear range of serpin A12 concentration (0.039– 10 ng/mL), detection limit of 0.020 ng/mL (S/N=3), and 0.031 ng/mL in solution buffer and plasma, respectively.Conclusion: The results indicate that this aptasensor has a high sensitivity, selectivity, stability, and acceptable reproducibility for detection of serpin A12 in diabetic patients.Keywords: electrochemical aptasensor, screen-printed carbon electrode, flower-like gold microstructures, label-free detection, serpin A12, diabetes biomarkersSalek Maghsoudi AHassani SRezaei Akmal MGanjali MRMirnia KNorouzi PAbdollahi MDove Medical Pressarticleelectrochemical aptasensorscreen-printed carbon electrodeflower-like gold microstructureslabel free detectionserpin a12diabetes biomarkersMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2219-2230 (2020) |
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electrochemical aptasensor screen-printed carbon electrode flower-like gold microstructures label free detection serpin a12 diabetes biomarkers Medicine (General) R5-920 |
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electrochemical aptasensor screen-printed carbon electrode flower-like gold microstructures label free detection serpin a12 diabetes biomarkers Medicine (General) R5-920 Salek Maghsoudi A Hassani S Rezaei Akmal M Ganjali MR Mirnia K Norouzi P Abdollahi M An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker |
| description |
Armin Salek Maghsoudi,1,* Shokoufeh Hassani,1,2,* Milad Rezaei Akmal,3 Mohammad Reza Ganjali,3,4 Kayvan Mirnia,5 Parviz Norouzi,3,4 Mohammad Abdollahi1,2 1Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 2Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran; 3Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran; 4Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran; 5Department of Neonatology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran*These authors contributed equally to this workCorrespondence: Mohammad AbdollahiToxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran 1417614411, IranTel +98 21 64122319Email mohammad@tums.ac.irPurpose: In the present study, a highly sensitive and simple electrochemical (EC) aptasensor for the detection of serpin A12 as a novel biomarker of diabetes was developed on a platform where flower-like gold microstructures (FLGMs) are electrodeposited onto a disposable screen-printed carbon electrode. Meanwhile, serpin A12-specific thiolated aptamer was covalently immobilized on the FLGMs.Methods: The electrochemical activity of a fabricated aptasensor under various conditions were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Aptamer concentration, deposition time, self-assembly time, and incubation time were optimized for assay of serpin A12. The differential pulse voltammetry (DPV) was implemented for quantitative detection of serpin A12 in K3 [Fe (CN) 6]/K4 [Fe (CN) 6] solution (redox probe).Results: The label-free aptasensor revealed a linear range of serpin A12 concentration (0.039– 10 ng/mL), detection limit of 0.020 ng/mL (S/N=3), and 0.031 ng/mL in solution buffer and plasma, respectively.Conclusion: The results indicate that this aptasensor has a high sensitivity, selectivity, stability, and acceptable reproducibility for detection of serpin A12 in diabetic patients.Keywords: electrochemical aptasensor, screen-printed carbon electrode, flower-like gold microstructures, label-free detection, serpin A12, diabetes biomarkers |
| format |
article |
| author |
Salek Maghsoudi A Hassani S Rezaei Akmal M Ganjali MR Mirnia K Norouzi P Abdollahi M |
| author_facet |
Salek Maghsoudi A Hassani S Rezaei Akmal M Ganjali MR Mirnia K Norouzi P Abdollahi M |
| author_sort |
Salek Maghsoudi A |
| title |
An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker |
| title_short |
An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker |
| title_full |
An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker |
| title_fullStr |
An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker |
| title_full_unstemmed |
An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker |
| title_sort |
electrochemical aptasensor platform based on flower-like gold microstructure-modified screen-printed carbon electrode for detection of serpin a12 as a type 2 diabetes biomarker |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/cdae864379d24aec847b1d1515e8d275 |
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