Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219

Abstract Clostridium botulinum is an anaerobic sporeforming bacterium that is notorious for producing a potent neurotoxin. Spores of C. botulinum can survive mild food processing treatments and subsequently germinate, multiply, produce toxin and cause botulism. Control of spore germination and outgr...

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Autores principales:	Charlien Clauwers, Cédric Lood, Bram Van den Bergh, Vera van Noort, Chris W. Michiels
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdb112d1d3154a2fa46d1f3f9b1cd11c
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spelling	oai:doaj.org-article:cdb112d1d3154a2fa46d1f3f9b1cd11c2021-12-02T15:05:37ZCanonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 1121910.1038/s41598-017-15839-y2045-2322https://doaj.org/article/cdb112d1d3154a2fa46d1f3f9b1cd11c2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15839-yhttps://doaj.org/toc/2045-2322Abstract Clostridium botulinum is an anaerobic sporeforming bacterium that is notorious for producing a potent neurotoxin. Spores of C. botulinum can survive mild food processing treatments and subsequently germinate, multiply, produce toxin and cause botulism. Control of spore germination and outgrowth is therefore essential for the safety of mildly processed foods. However, little is known about the process of spore germination in group II C. botulinum (gIICb), which are a major concern in chilled foods because they are psychrotrophic. The classical model of spore germination states that germination is triggered by the binding of a germinant molecule to a cognate germinant receptor. Remarkably, unlike many other sporeformers, gIICb has only one predicted canonical germinant receptor although it responds to multiple germinants. Therefore, we deleted the gerBAC locus that encodes this germinant receptor to determine its role in germination. Surprisingly, the deletion did not affect germination by any of the nutrient germinants, nor by the non-nutrient dodecylamine. We conclude that one or more other, so far unidentified, germinant receptors must be responsible for nutrient induced germination in gIICb. Furthermore, the gerBAC locus was strongly conserved with intact open reading frames in 159 gIICb genomes, suggesting that it has nevertheless an important function.Charlien ClauwersCédric LoodBram Van den BerghVera van NoortChris W. MichielsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charlien Clauwers Cédric Lood Bram Van den Bergh Vera van Noort Chris W. Michiels Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219
description	Abstract Clostridium botulinum is an anaerobic sporeforming bacterium that is notorious for producing a potent neurotoxin. Spores of C. botulinum can survive mild food processing treatments and subsequently germinate, multiply, produce toxin and cause botulism. Control of spore germination and outgrowth is therefore essential for the safety of mildly processed foods. However, little is known about the process of spore germination in group II C. botulinum (gIICb), which are a major concern in chilled foods because they are psychrotrophic. The classical model of spore germination states that germination is triggered by the binding of a germinant molecule to a cognate germinant receptor. Remarkably, unlike many other sporeformers, gIICb has only one predicted canonical germinant receptor although it responds to multiple germinants. Therefore, we deleted the gerBAC locus that encodes this germinant receptor to determine its role in germination. Surprisingly, the deletion did not affect germination by any of the nutrient germinants, nor by the non-nutrient dodecylamine. We conclude that one or more other, so far unidentified, germinant receptors must be responsible for nutrient induced germination in gIICb. Furthermore, the gerBAC locus was strongly conserved with intact open reading frames in 159 gIICb genomes, suggesting that it has nevertheless an important function.
format	article
author	Charlien Clauwers Cédric Lood Bram Van den Bergh Vera van Noort Chris W. Michiels
author_facet	Charlien Clauwers Cédric Lood Bram Van den Bergh Vera van Noort Chris W. Michiels
author_sort	Charlien Clauwers
title	Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219
title_short	Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219
title_full	Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219
title_fullStr	Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219
title_full_unstemmed	Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219
title_sort	canonical germinant receptor is dispensable for spore germination in clostridium botulinum group ii strain nctc 11219
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cdb112d1d3154a2fa46d1f3f9b1cd11c
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Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219

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