Analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria

Abstract Due to tetracycline abuse, the safe bifidobacteria in the human gastrointestinal intestinal tract (GIT) may serve as a reservoir of tetracycline resistance genes. In the present investigation of 92 bifidobacterial strains originating from the human GIT, tetracycline resistance in 29 strains...

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Autores principales:	Na Wang, Xiaomin Hang, Min Zhang, Xianglong Liu, Hong Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdb46fa0ef4d4ca294b3fb824b4255cb
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spelling	oai:doaj.org-article:cdb46fa0ef4d4ca294b3fb824b4255cb2021-12-02T16:06:07ZAnalysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria10.1038/s41598-017-06595-02045-2322https://doaj.org/article/cdb46fa0ef4d4ca294b3fb824b4255cb2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06595-0https://doaj.org/toc/2045-2322Abstract Due to tetracycline abuse, the safe bifidobacteria in the human gastrointestinal intestinal tract (GIT) may serve as a reservoir of tetracycline resistance genes. In the present investigation of 92 bifidobacterial strains originating from the human GIT, tetracycline resistance in 29 strains was mediated by the tet(W), tet(O) or tet(S) gene, and this is the first report of tet(O)- and tet(S)-mediated tetracycline resistance in bifidobacteria. Antibiotic resistance genes harbored by bifidobacteria are transferred from other bacteria. However, the characteristics of the spread and integration of tetracycline resistance genes into the human intestinal bifidobacteria chromosome are poorly understood. Here, conserved sequences were identified in bifidobacterial strains positive for tet(W), tet(O), or tet(S), including the tet(W), tet(O), or tet(S) and their partial flanking sequences, which exhibited identity with the sequences in multiple human intestinal pathogens, and genes encoding 23 S rRNA, an ATP transporter, a Cpp protein, and a membrane-spanning protein were flanking by the 1920-bp tet(W), 1920-bp tet(O), 1800-bp tet(O) and 252-bp tet(S) in bifidobacteria, respectively. These findings suggest that tetracycline resistance genes harbored by human intestinal bifidobacteria might initially be transferred from pathogens and that each kind of tetracycline resistance gene might tend to insert in the vicinity of specific bifidobacteria genes.Na WangXiaomin HangMin ZhangXianglong LiuHong YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Na Wang Xiaomin Hang Min Zhang Xianglong Liu Hong Yang Analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria
description	Abstract Due to tetracycline abuse, the safe bifidobacteria in the human gastrointestinal intestinal tract (GIT) may serve as a reservoir of tetracycline resistance genes. In the present investigation of 92 bifidobacterial strains originating from the human GIT, tetracycline resistance in 29 strains was mediated by the tet(W), tet(O) or tet(S) gene, and this is the first report of tet(O)- and tet(S)-mediated tetracycline resistance in bifidobacteria. Antibiotic resistance genes harbored by bifidobacteria are transferred from other bacteria. However, the characteristics of the spread and integration of tetracycline resistance genes into the human intestinal bifidobacteria chromosome are poorly understood. Here, conserved sequences were identified in bifidobacterial strains positive for tet(W), tet(O), or tet(S), including the tet(W), tet(O), or tet(S) and their partial flanking sequences, which exhibited identity with the sequences in multiple human intestinal pathogens, and genes encoding 23 S rRNA, an ATP transporter, a Cpp protein, and a membrane-spanning protein were flanking by the 1920-bp tet(W), 1920-bp tet(O), 1800-bp tet(O) and 252-bp tet(S) in bifidobacteria, respectively. These findings suggest that tetracycline resistance genes harbored by human intestinal bifidobacteria might initially be transferred from pathogens and that each kind of tetracycline resistance gene might tend to insert in the vicinity of specific bifidobacteria genes.
format	article
author	Na Wang Xiaomin Hang Min Zhang Xianglong Liu Hong Yang
author_facet	Na Wang Xiaomin Hang Min Zhang Xianglong Liu Hong Yang
author_sort	Na Wang
title	Analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria
title_short	Analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria
title_full	Analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria
title_fullStr	Analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria
title_full_unstemmed	Analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria
title_sort	analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cdb46fa0ef4d4ca294b3fb824b4255cb
work_keys_str_mv	AT nawang analysisofnewlydetectedtetracyclineresistancegenesandtheirflankingsequencesinhumanintestinalbifidobacteria AT xiaominhang analysisofnewlydetectedtetracyclineresistancegenesandtheirflankingsequencesinhumanintestinalbifidobacteria AT minzhang analysisofnewlydetectedtetracyclineresistancegenesandtheirflankingsequencesinhumanintestinalbifidobacteria AT xianglongliu analysisofnewlydetectedtetracyclineresistancegenesandtheirflankingsequencesinhumanintestinalbifidobacteria AT hongyang analysisofnewlydetectedtetracyclineresistancegenesandtheirflankingsequencesinhumanintestinalbifidobacteria
_version_	1718385114806747136

Analysis of newly detected tetracycline resistance genes and their flanking sequences in human intestinal bifidobacteria

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