Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy

Abstract We present the first direct nuclear magnetic resonance (NMR) evidence of enhanced entry of Ca2+ ions into human erythrocytes (red blood cells; RBCs), when these cells are mechanically distorted. For this we loaded the RBCs with the fluorinated Ca2+ chelator, 1,2-bis(2-amino-5-fluorophenoxy)...

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Autores principales:	Philip W. Kuchel, Konstantin Romanenko, Dmitry Shishmarev, Petrik Galvosas, Charles D. Cox
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdb8c935e89246b4977bec8744a49a67
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spelling	oai:doaj.org-article:cdb8c935e89246b4977bec8744a49a672021-12-02T13:30:18ZEnhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy10.1038/s41598-021-83044-z2045-2322https://doaj.org/article/cdb8c935e89246b4977bec8744a49a672021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83044-zhttps://doaj.org/toc/2045-2322Abstract We present the first direct nuclear magnetic resonance (NMR) evidence of enhanced entry of Ca2+ ions into human erythrocytes (red blood cells; RBCs), when these cells are mechanically distorted. For this we loaded the RBCs with the fluorinated Ca2+ chelator, 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N′,N′-tetraacetic acid (5FBAPTA), and recorded 19F NMR spectra. The RBCs were suspended in gelatin gel in a special stretching/compression apparatus. The 5FBAPTA was loaded into the cells as the tetraacetoxymethyl ester; and 13C NMR spectroscopy with [1,6-13C]d-glucose as substrate showed active glycolysis albeit at a reduced rate in cell suspensions and gels. The enhancement of Ca2+ influx is concluded to be via the mechanosensitive cation channel Piezo1. The increased rate of influx brought about by the activator of Piezo1, 2-[5-[[(2,6-dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]-pyrazine (Yoda1) supported this conclusion; while the specificity of the cation-sensing by 5FBAPTA was confirmed by using the Ca2+ ionophore, A23187.Philip W. KuchelKonstantin RomanenkoDmitry ShishmarevPetrik GalvosasCharles D. CoxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Philip W. Kuchel Konstantin Romanenko Dmitry Shishmarev Petrik Galvosas Charles D. Cox Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy
description	Abstract We present the first direct nuclear magnetic resonance (NMR) evidence of enhanced entry of Ca2+ ions into human erythrocytes (red blood cells; RBCs), when these cells are mechanically distorted. For this we loaded the RBCs with the fluorinated Ca2+ chelator, 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N′,N′-tetraacetic acid (5FBAPTA), and recorded 19F NMR spectra. The RBCs were suspended in gelatin gel in a special stretching/compression apparatus. The 5FBAPTA was loaded into the cells as the tetraacetoxymethyl ester; and 13C NMR spectroscopy with [1,6-13C]d-glucose as substrate showed active glycolysis albeit at a reduced rate in cell suspensions and gels. The enhancement of Ca2+ influx is concluded to be via the mechanosensitive cation channel Piezo1. The increased rate of influx brought about by the activator of Piezo1, 2-[5-[[(2,6-dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]-pyrazine (Yoda1) supported this conclusion; while the specificity of the cation-sensing by 5FBAPTA was confirmed by using the Ca2+ ionophore, A23187.
format	article
author	Philip W. Kuchel Konstantin Romanenko Dmitry Shishmarev Petrik Galvosas Charles D. Cox
author_facet	Philip W. Kuchel Konstantin Romanenko Dmitry Shishmarev Petrik Galvosas Charles D. Cox
author_sort	Philip W. Kuchel
title	Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy
title_short	Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy
title_full	Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy
title_fullStr	Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy
title_full_unstemmed	Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy
title_sort	enhanced ca2+ influx in mechanically distorted erythrocytes measured with 19f nuclear magnetic resonance spectroscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cdb8c935e89246b4977bec8744a49a67
work_keys_str_mv	AT philipwkuchel enhancedca2influxinmechanicallydistortederythrocytesmeasuredwith19fnuclearmagneticresonancespectroscopy AT konstantinromanenko enhancedca2influxinmechanicallydistortederythrocytesmeasuredwith19fnuclearmagneticresonancespectroscopy AT dmitryshishmarev enhancedca2influxinmechanicallydistortederythrocytesmeasuredwith19fnuclearmagneticresonancespectroscopy AT petrikgalvosas enhancedca2influxinmechanicallydistortederythrocytesmeasuredwith19fnuclearmagneticresonancespectroscopy AT charlesdcox enhancedca2influxinmechanicallydistortederythrocytesmeasuredwith19fnuclearmagneticresonancespectroscopy
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Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy

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