Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins

Abstract The membrane insertase YidC inserts newly synthesized proteins by its hydrophobic slide consisting of the two transmembrane (TM) segments TM3 and TM5. Mutations in this part of the protein affect the insertion of the client proteins. We show here that a quintuple mutation, termed YidC-5S, i...

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Autores principales:	Anja Steudle, Dirk Spann, Eva Pross, Sri Karthika Shanmugam, Ross E. Dalbey, Andreas Kuhn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdbe565b07bf43628edf13ae8296fd9e
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spelling	oai:doaj.org-article:cdbe565b07bf43628edf13ae8296fd9e2021-12-02T12:11:17ZMolecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins10.1038/s41598-021-83224-x2045-2322https://doaj.org/article/cdbe565b07bf43628edf13ae8296fd9e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83224-xhttps://doaj.org/toc/2045-2322Abstract The membrane insertase YidC inserts newly synthesized proteins by its hydrophobic slide consisting of the two transmembrane (TM) segments TM3 and TM5. Mutations in this part of the protein affect the insertion of the client proteins. We show here that a quintuple mutation, termed YidC-5S, inhibits the insertion of the subunit a of the FoF1 ATP synthase but has no effect on the insertion of the Sec-independent M13 procoat protein and the C-tail protein SciP. Further investigations show that the interaction of YidC-5S with SecY is inhibited. The purified and fluorescently labeled YidC-5S did not approach SecYEG when both were co-reconstituted in proteoliposomes in contrast to the co-reconstituted YidC wild type. These results suggest that TM3 and TM5 are involved in the formation of a common YidC-SecYEG complex that is required for the insertion of Sec/YidC-dependent client proteins.Anja SteudleDirk SpannEva ProssSri Karthika ShanmugamRoss E. DalbeyAndreas KuhnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anja Steudle Dirk Spann Eva Pross Sri Karthika Shanmugam Ross E. Dalbey Andreas Kuhn Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins
description	Abstract The membrane insertase YidC inserts newly synthesized proteins by its hydrophobic slide consisting of the two transmembrane (TM) segments TM3 and TM5. Mutations in this part of the protein affect the insertion of the client proteins. We show here that a quintuple mutation, termed YidC-5S, inhibits the insertion of the subunit a of the FoF1 ATP synthase but has no effect on the insertion of the Sec-independent M13 procoat protein and the C-tail protein SciP. Further investigations show that the interaction of YidC-5S with SecY is inhibited. The purified and fluorescently labeled YidC-5S did not approach SecYEG when both were co-reconstituted in proteoliposomes in contrast to the co-reconstituted YidC wild type. These results suggest that TM3 and TM5 are involved in the formation of a common YidC-SecYEG complex that is required for the insertion of Sec/YidC-dependent client proteins.
format	article
author	Anja Steudle Dirk Spann Eva Pross Sri Karthika Shanmugam Ross E. Dalbey Andreas Kuhn
author_facet	Anja Steudle Dirk Spann Eva Pross Sri Karthika Shanmugam Ross E. Dalbey Andreas Kuhn
author_sort	Anja Steudle
title	Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins
title_short	Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins
title_full	Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins
title_fullStr	Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins
title_full_unstemmed	Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins
title_sort	molecular communication of the membrane insertase yidc with translocase secyeg affects client proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cdbe565b07bf43628edf13ae8296fd9e
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Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins

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