Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects

Abstract Epimedium brevicornum Maxim has a long history of use in the treatment of estrogen deficiency-related diseases. However, the chemical constituents and mechanism of action of this medicinal plant are not fully understood. In the present study, we isolated four new isoprenylated flavonoid gly...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fu Li, Bao-Wen Du, Dan-Feng Lu, Wen-Xuan Wu, Kanjana Wongkrajang, Lun Wang, Wen-Chen Pu, Chang-Lu Liu, Han-Wei Liu, Ming-Kui Wang, Fei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdbf79c242b5456e9d71bb62e04b9411
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cdbf79c242b5456e9d71bb62e04b9411
record_format	dspace
spelling	oai:doaj.org-article:cdbf79c242b5456e9d71bb62e04b94112021-12-02T12:32:07ZFlavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects10.1038/s41598-017-08203-72045-2322https://doaj.org/article/cdbf79c242b5456e9d71bb62e04b94112017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08203-7https://doaj.org/toc/2045-2322Abstract Epimedium brevicornum Maxim has a long history of use in the treatment of estrogen deficiency-related diseases. However, the chemical constituents and mechanism of action of this medicinal plant are not fully understood. In the present study, we isolated four new isoprenylated flavonoid glycosides, as well as 16 known flavonoids (13 isoprenylated flavonoids), from this plant. The chemical structures of the new flavonoid glycosides were elucidated by extensive spectroscopic analysis. The new compounds 1–4 were potent promoters of estrogen biosynthesis in human ovarian granulosa-like KGN cells. ZW1, an isoprenylated flavonoid analogue and a specific inhibitor of phosphodiesterase 5 (PDE5), was synthesized and used to explore the mechanism of the isoprenylated analogues on estrogen biosynthesis. ZW1 treatment increased estrogen production by upregulation of aromatase mRNA and protein expression. ZW1 increased the phosphorylation of cAMP response element-binding protein (CREB). Further study showed that the inhibition of PDE5 by ZW1 increased estrogen biosynthesis partly through suppression of phosphodiesterase 3 (PDE3). Our results suggested that the isoprenylated flavonoids from E. brevicornum may produce beneficial health effects through the promotion of estrogen biosynthesis. PDE5 warrants further investigation as a new therapeutic target for estrogen biosynthesis in the prevention and treatment of estrogen-deficiency related diseases.Fu LiBao-Wen DuDan-Feng LuWen-Xuan WuKanjana WongkrajangLun WangWen-Chen PuChang-Lu LiuHan-Wei LiuMing-Kui WangFei WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fu Li Bao-Wen Du Dan-Feng Lu Wen-Xuan Wu Kanjana Wongkrajang Lun Wang Wen-Chen Pu Chang-Lu Liu Han-Wei Liu Ming-Kui Wang Fei Wang Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects
description	Abstract Epimedium brevicornum Maxim has a long history of use in the treatment of estrogen deficiency-related diseases. However, the chemical constituents and mechanism of action of this medicinal plant are not fully understood. In the present study, we isolated four new isoprenylated flavonoid glycosides, as well as 16 known flavonoids (13 isoprenylated flavonoids), from this plant. The chemical structures of the new flavonoid glycosides were elucidated by extensive spectroscopic analysis. The new compounds 1–4 were potent promoters of estrogen biosynthesis in human ovarian granulosa-like KGN cells. ZW1, an isoprenylated flavonoid analogue and a specific inhibitor of phosphodiesterase 5 (PDE5), was synthesized and used to explore the mechanism of the isoprenylated analogues on estrogen biosynthesis. ZW1 treatment increased estrogen production by upregulation of aromatase mRNA and protein expression. ZW1 increased the phosphorylation of cAMP response element-binding protein (CREB). Further study showed that the inhibition of PDE5 by ZW1 increased estrogen biosynthesis partly through suppression of phosphodiesterase 3 (PDE3). Our results suggested that the isoprenylated flavonoids from E. brevicornum may produce beneficial health effects through the promotion of estrogen biosynthesis. PDE5 warrants further investigation as a new therapeutic target for estrogen biosynthesis in the prevention and treatment of estrogen-deficiency related diseases.
format	article
author	Fu Li Bao-Wen Du Dan-Feng Lu Wen-Xuan Wu Kanjana Wongkrajang Lun Wang Wen-Chen Pu Chang-Lu Liu Han-Wei Liu Ming-Kui Wang Fei Wang
author_facet	Fu Li Bao-Wen Du Dan-Feng Lu Wen-Xuan Wu Kanjana Wongkrajang Lun Wang Wen-Chen Pu Chang-Lu Liu Han-Wei Liu Ming-Kui Wang Fei Wang
author_sort	Fu Li
title	Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects
title_short	Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects
title_full	Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects
title_fullStr	Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects
title_full_unstemmed	Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects
title_sort	flavonoid glycosides isolated from epimedium brevicornum and their estrogen biosynthesis-promoting effects
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cdbf79c242b5456e9d71bb62e04b9411
work_keys_str_mv	AT fuli flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT baowendu flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT danfenglu flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT wenxuanwu flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT kanjanawongkrajang flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT lunwang flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT wenchenpu flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT changluliu flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT hanweiliu flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT mingkuiwang flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects AT feiwang flavonoidglycosidesisolatedfromepimediumbrevicornumandtheirestrogenbiosynthesispromotingeffects
_version_	1718394184393555968

Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects

Ejemplares similares