Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.

<h4>Background</h4>Granulocytes and monocytes/macrophages differentiate from common myeloid progenitor cells. Granulocyte colony-stimulating factor (G-CSF) and CD137 (4-1BB, TNFRSF9) are growth and differentiation factors that induce granulocyte and macrophage survival and differentiatio...

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Autores principales:	Dongsheng Jiang, Herbert Schwarz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdc2469932774f98a84350d462ec7cd7
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spelling	oai:doaj.org-article:cdc2469932774f98a84350d462ec7cd72021-11-18T07:01:46ZRegulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.1932-620310.1371/journal.pone.0015565https://doaj.org/article/cdc2469932774f98a84350d462ec7cd72010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21179444/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Granulocytes and monocytes/macrophages differentiate from common myeloid progenitor cells. Granulocyte colony-stimulating factor (G-CSF) and CD137 (4-1BB, TNFRSF9) are growth and differentiation factors that induce granulocyte and macrophage survival and differentiation, respectively. This study describes the influence of G-CSF and recombinant CD137-Fc protein on myelopoiesis.<h4>Methodology/principal findings</h4>Both, G-CSF and CD137 protein support proliferation and survival of murine bone marrow cells. G-CSF enhances granulocyte numbers while CD137 protein enhances macrophage numbers. Both growth factors together give rise to more cells than each factor alone. Titration of G-CSF and CD137 protein dose-dependently changes the granulocyte/macrophage ratio in bone marrow cells. Both factors individually induce proliferation of hematopoietic progenitor cells (lin-, c-kit+) and differentiation to granulocytes and macrophages, respectively. The combination of G-CSF and CD137 protein further increases proliferation, and results in a higher number of macrophages than CD137 protein alone, and a lower number of granulocytes than G-CSF alone demonstrating that CD137 protein-induced monocytic differentiation is dominant over G-CSF-induced granulocytic differentiation. CD137 protein induces monocytic differentiation even in early hematopoietic progenitor cells, the common myeloid progenitors and the granulocyte macrophage progenitors.<h4>Conclusions/significance</h4>This study confirms earlier data on the regulation of myelopoiesis by CD137 receptor - ligand interaction, and extends them by demonstrating the restriction of this growth promoting influence to the monocytic lineage.Dongsheng JiangHerbert SchwarzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15565 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dongsheng Jiang Herbert Schwarz Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.
description	<h4>Background</h4>Granulocytes and monocytes/macrophages differentiate from common myeloid progenitor cells. Granulocyte colony-stimulating factor (G-CSF) and CD137 (4-1BB, TNFRSF9) are growth and differentiation factors that induce granulocyte and macrophage survival and differentiation, respectively. This study describes the influence of G-CSF and recombinant CD137-Fc protein on myelopoiesis.<h4>Methodology/principal findings</h4>Both, G-CSF and CD137 protein support proliferation and survival of murine bone marrow cells. G-CSF enhances granulocyte numbers while CD137 protein enhances macrophage numbers. Both growth factors together give rise to more cells than each factor alone. Titration of G-CSF and CD137 protein dose-dependently changes the granulocyte/macrophage ratio in bone marrow cells. Both factors individually induce proliferation of hematopoietic progenitor cells (lin-, c-kit+) and differentiation to granulocytes and macrophages, respectively. The combination of G-CSF and CD137 protein further increases proliferation, and results in a higher number of macrophages than CD137 protein alone, and a lower number of granulocytes than G-CSF alone demonstrating that CD137 protein-induced monocytic differentiation is dominant over G-CSF-induced granulocytic differentiation. CD137 protein induces monocytic differentiation even in early hematopoietic progenitor cells, the common myeloid progenitors and the granulocyte macrophage progenitors.<h4>Conclusions/significance</h4>This study confirms earlier data on the regulation of myelopoiesis by CD137 receptor - ligand interaction, and extends them by demonstrating the restriction of this growth promoting influence to the monocytic lineage.
format	article
author	Dongsheng Jiang Herbert Schwarz
author_facet	Dongsheng Jiang Herbert Schwarz
author_sort	Dongsheng Jiang
title	Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.
title_short	Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.
title_full	Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.
title_fullStr	Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.
title_full_unstemmed	Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.
title_sort	regulation of granulocyte and macrophage populations of murine bone marrow cells by g-csf and cd137 protein.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/cdc2469932774f98a84350d462ec7cd7
work_keys_str_mv	AT dongshengjiang regulationofgranulocyteandmacrophagepopulationsofmurinebonemarrowcellsbygcsfandcd137protein AT herbertschwarz regulationofgranulocyteandmacrophagepopulationsofmurinebonemarrowcellsbygcsfandcd137protein
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Regulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.

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