A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis

Rafael Salmazi, Giovana Calixto, Jéssica Bernegossi, Matheus Aparecido dos Santos Ramos, Taís Maria Bauab, Marlus Chorilli School of Pharmaceutical Sciences, UNESP – Sao Paulo State University, Campus Araraquara, Department of Drugs and Medicines, Araraquara, Sao Paul...

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Autores principales:	Salmazi R, Calixto G, Bernegossi J, Ramos MA, Bauab TM, Chorilli M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cdc61fb3abb94105910e3bd2a229279a
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spelling	oai:doaj.org-article:cdc61fb3abb94105910e3bd2a229279a2021-12-02T04:23:12ZA curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis1178-2013https://doaj.org/article/cdc61fb3abb94105910e3bd2a229279a2015-07-01T00:00:00Zhttp://www.dovepress.com/a-curcumin-loaded-liquid-crystal-precursor-mucoadhesive-system-for-the-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rafael Salmazi, Giovana Calixto, Jéssica Bernegossi, Matheus Aparecido dos Santos Ramos, Taís Maria Bauab, Marlus Chorilli School of Pharmaceutical Sciences, UNESP – Sao Paulo State University, Campus Araraquara, Department of Drugs and Medicines, Araraquara, Sao Paulo, Brazil Abstract: Women often develop vaginal infections that are caused primarily by organisms of the genus Candida. The current treatments of vaginal candidiasis usually involve azole-based antifungals, though fungal resistance to these compounds has become prevalent. Therefore, much attention has been given to molecules with antifungal properties from natural sources, such as curcumin (CUR). However, CUR has poor solubility in aqueous solvents and poor oral bioavailability. This study attempted to overcome this problem by developing, characterizing, and evaluating the in vitro antifungal action of a CUR-loaded liquid crystal precursor mucoadhesive system (LCPM) for vaginal administration. A low-viscosity LCPM (F) consisting of 40% wt/wt polyoxpropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, 50% wt/wt oleic acid, and 10% wt/wt chitosan dispersion at 0.5% with the addition of 16% poloxamer 407 was developed to take advantage of the lyotropic phase behavior of this formulation. Notably, F could transform into liquid crystal systems when diluted with artificial vaginal mucus at ratios of 1:3 and 1:1 (wt/wt), resulting in the formation of F30 and F100, respectively. Polarized light microscopy and rheological studies revealed that F behaved like an isotropic formulation, whereas F30 and F100 behaved like an anisotropic liquid crystalline system (LCS). Moreover, F30 and F100 presented higher mucoadhesion to porcine vaginal mucosa than F. The analysis of the in vitro activity against Candida albicans revealed that CUR-loaded F was more potent against standard and clinical strains compared with a CUR solution. Therefore, the vaginal administration of CUR-loaded LCPMs represents a promising platform for the treatment of vaginal candidiasis. Keywords: nanostructured drug delivery systems, liquid crystalline systems, mucoadhesive polymers, vaginal administration, Candida albicans Salmazi RCalixto GBernegossi JRamos MABauab TMChorilli MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 4815-4824 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Salmazi R Calixto G Bernegossi J Ramos MA Bauab TM Chorilli M A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis
description	Rafael Salmazi, Giovana Calixto, Jéssica Bernegossi, Matheus Aparecido dos Santos Ramos, Taís Maria Bauab, Marlus Chorilli School of Pharmaceutical Sciences, UNESP – Sao Paulo State University, Campus Araraquara, Department of Drugs and Medicines, Araraquara, Sao Paulo, Brazil Abstract: Women often develop vaginal infections that are caused primarily by organisms of the genus Candida. The current treatments of vaginal candidiasis usually involve azole-based antifungals, though fungal resistance to these compounds has become prevalent. Therefore, much attention has been given to molecules with antifungal properties from natural sources, such as curcumin (CUR). However, CUR has poor solubility in aqueous solvents and poor oral bioavailability. This study attempted to overcome this problem by developing, characterizing, and evaluating the in vitro antifungal action of a CUR-loaded liquid crystal precursor mucoadhesive system (LCPM) for vaginal administration. A low-viscosity LCPM (F) consisting of 40% wt/wt polyoxpropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, 50% wt/wt oleic acid, and 10% wt/wt chitosan dispersion at 0.5% with the addition of 16% poloxamer 407 was developed to take advantage of the lyotropic phase behavior of this formulation. Notably, F could transform into liquid crystal systems when diluted with artificial vaginal mucus at ratios of 1:3 and 1:1 (wt/wt), resulting in the formation of F30 and F100, respectively. Polarized light microscopy and rheological studies revealed that F behaved like an isotropic formulation, whereas F30 and F100 behaved like an anisotropic liquid crystalline system (LCS). Moreover, F30 and F100 presented higher mucoadhesion to porcine vaginal mucosa than F. The analysis of the in vitro activity against Candida albicans revealed that CUR-loaded F was more potent against standard and clinical strains compared with a CUR solution. Therefore, the vaginal administration of CUR-loaded LCPMs represents a promising platform for the treatment of vaginal candidiasis. Keywords: nanostructured drug delivery systems, liquid crystalline systems, mucoadhesive polymers, vaginal administration, Candida albicans
format	article
author	Salmazi R Calixto G Bernegossi J Ramos MA Bauab TM Chorilli M
author_facet	Salmazi R Calixto G Bernegossi J Ramos MA Bauab TM Chorilli M
author_sort	Salmazi R
title	A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis
title_short	A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis
title_full	A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis
title_fullStr	A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis
title_full_unstemmed	A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis
title_sort	curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/cdc61fb3abb94105910e3bd2a229279a
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A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis

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