Comparative genomics and transcriptomics of Propionibacterium acnes.

The anaerobic gram-positive bacterium Propionibacterium acnes is a human skin commensal that is occasionally associated with inflammatory diseases. Recent work has indicated that evolutionary distinct lineages of P. acnes play etiologic roles in disease while others are associated with maintenance o...

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Autores principales:	Elzbieta Brzuszkiewicz, January Weiner, Antje Wollherr, Andrea Thürmer, Jennifer Hüpeden, Hans B Lomholt, Mogens Kilian, Gerhard Gottschalk, Rolf Daniel, Hans-Joachim Mollenkopf, Thomas F Meyer, Holger Brüggemann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdc6bf5856fc4d4e96720a1133c10698
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spelling	oai:doaj.org-article:cdc6bf5856fc4d4e96720a1133c106982021-11-18T06:51:11ZComparative genomics and transcriptomics of Propionibacterium acnes.1932-620310.1371/journal.pone.0021581https://doaj.org/article/cdc6bf5856fc4d4e96720a1133c106982011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21738717/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The anaerobic gram-positive bacterium Propionibacterium acnes is a human skin commensal that is occasionally associated with inflammatory diseases. Recent work has indicated that evolutionary distinct lineages of P. acnes play etiologic roles in disease while others are associated with maintenance of skin homeostasis. To shed light on the molecular basis for differential strain properties, we carried out genomic and transcriptomic analysis of distinct P. acnes strains. We sequenced the genome of the P. acnes strain 266, a type I-1a strain. Comparative genome analysis of strain 266 and four other P. acnes strains revealed that overall genome plasticity is relatively low; however, a number of island-like genomic regions, encoding a variety of putative virulence-associated and fitness traits differ between phylotypes, as judged from PCR analysis of a collection of P. acnes strains. Comparative transcriptome analysis of strains KPA171202 (type I-2) and 266 during exponential growth revealed inter-strain differences in gene expression of transport systems and metabolic pathways. In addition, transcript levels of genes encoding possible virulence factors such as dermatan-sulphate adhesin, polyunsaturated fatty acid isomerase, iron acquisition protein HtaA and lipase GehA were upregulated in strain 266. We investigated differential gene expression during exponential and stationary growth phases. Genes encoding components of the energy-conserving respiratory chain as well as secreted and virulence-associated factors were transcribed during the exponential phase, while the stationary growth phase was characterized by upregulation of genes involved in stress responses and amino acid metabolism. Our data highlight the genomic basis for strain diversity and identify, for the first time, the actively transcribed part of the genome, underlining the important role growth status plays in the inflammation-inducing activity of P. acnes. We argue that the disease-causing potential of different P. acnes strains is not only determined by the phylotype-specific genome content but also by variable gene expression.Elzbieta BrzuszkiewiczJanuary WeinerAntje WollherrAndrea ThürmerJennifer HüpedenHans B LomholtMogens KilianGerhard GottschalkRolf DanielHans-Joachim MollenkopfThomas F MeyerHolger BrüggemannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e21581 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elzbieta Brzuszkiewicz January Weiner Antje Wollherr Andrea Thürmer Jennifer Hüpeden Hans B Lomholt Mogens Kilian Gerhard Gottschalk Rolf Daniel Hans-Joachim Mollenkopf Thomas F Meyer Holger Brüggemann Comparative genomics and transcriptomics of Propionibacterium acnes.
description	The anaerobic gram-positive bacterium Propionibacterium acnes is a human skin commensal that is occasionally associated with inflammatory diseases. Recent work has indicated that evolutionary distinct lineages of P. acnes play etiologic roles in disease while others are associated with maintenance of skin homeostasis. To shed light on the molecular basis for differential strain properties, we carried out genomic and transcriptomic analysis of distinct P. acnes strains. We sequenced the genome of the P. acnes strain 266, a type I-1a strain. Comparative genome analysis of strain 266 and four other P. acnes strains revealed that overall genome plasticity is relatively low; however, a number of island-like genomic regions, encoding a variety of putative virulence-associated and fitness traits differ between phylotypes, as judged from PCR analysis of a collection of P. acnes strains. Comparative transcriptome analysis of strains KPA171202 (type I-2) and 266 during exponential growth revealed inter-strain differences in gene expression of transport systems and metabolic pathways. In addition, transcript levels of genes encoding possible virulence factors such as dermatan-sulphate adhesin, polyunsaturated fatty acid isomerase, iron acquisition protein HtaA and lipase GehA were upregulated in strain 266. We investigated differential gene expression during exponential and stationary growth phases. Genes encoding components of the energy-conserving respiratory chain as well as secreted and virulence-associated factors were transcribed during the exponential phase, while the stationary growth phase was characterized by upregulation of genes involved in stress responses and amino acid metabolism. Our data highlight the genomic basis for strain diversity and identify, for the first time, the actively transcribed part of the genome, underlining the important role growth status plays in the inflammation-inducing activity of P. acnes. We argue that the disease-causing potential of different P. acnes strains is not only determined by the phylotype-specific genome content but also by variable gene expression.
format	article
author	Elzbieta Brzuszkiewicz January Weiner Antje Wollherr Andrea Thürmer Jennifer Hüpeden Hans B Lomholt Mogens Kilian Gerhard Gottschalk Rolf Daniel Hans-Joachim Mollenkopf Thomas F Meyer Holger Brüggemann
author_facet	Elzbieta Brzuszkiewicz January Weiner Antje Wollherr Andrea Thürmer Jennifer Hüpeden Hans B Lomholt Mogens Kilian Gerhard Gottschalk Rolf Daniel Hans-Joachim Mollenkopf Thomas F Meyer Holger Brüggemann
author_sort	Elzbieta Brzuszkiewicz
title	Comparative genomics and transcriptomics of Propionibacterium acnes.
title_short	Comparative genomics and transcriptomics of Propionibacterium acnes.
title_full	Comparative genomics and transcriptomics of Propionibacterium acnes.
title_fullStr	Comparative genomics and transcriptomics of Propionibacterium acnes.
title_full_unstemmed	Comparative genomics and transcriptomics of Propionibacterium acnes.
title_sort	comparative genomics and transcriptomics of propionibacterium acnes.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/cdc6bf5856fc4d4e96720a1133c10698
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Comparative genomics and transcriptomics of Propionibacterium acnes.

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