Uncovering Wolbachia diversity upon artificial host transfer.

The common endosymbiotic Wolbachia bacteria influence arthropod hosts in multiple ways. They are mostly recognized for their manipulations of host reproduction, yet, more recent studies demonstrate that Wolbachia also impact host behavior, metabolic pathways and immunity. Besides their biological an...

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Autores principales:	Daniela I Schneider, Markus Riegler, Wolfgang Arthofer, Hervé Merçot, Christian Stauffer, Wolfgang J Miller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdcdf97f3c754e958bfa4be5da63af85
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spelling	oai:doaj.org-article:cdcdf97f3c754e958bfa4be5da63af852021-11-18T08:41:01ZUncovering Wolbachia diversity upon artificial host transfer.1932-620310.1371/journal.pone.0082402https://doaj.org/article/cdcdf97f3c754e958bfa4be5da63af852013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24376534/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The common endosymbiotic Wolbachia bacteria influence arthropod hosts in multiple ways. They are mostly recognized for their manipulations of host reproduction, yet, more recent studies demonstrate that Wolbachia also impact host behavior, metabolic pathways and immunity. Besides their biological and evolutionary roles, Wolbachia are new potential biological control agents for pest and vector management. Importantly, Wolbachia-based control strategies require controlled symbiont transfer between host species and predictable outcomes of novel Wolbachia-host associations. Theoretically, this artificial horizontal transfer could inflict genetic changes within transferred Wolbachia populations. This could be facilitated through de novo mutations in the novel recipient host or changes of haplotype frequencies of polymorphic Wolbachia populations when transferred from donor to recipient hosts. Here we show that Wolbachia resident in the European cherry fruit fly, Rhagoletis cerasi, exhibit ancestral and cryptic sequence polymorphism in three symbiont genes, which are exposed upon microinjection into the new hosts Drosophila simulans and Ceratitis capitata. Our analyses of Wolbachia in microinjected D. simulans over 150 generations after microinjection uncovered infections with multiple Wolbachia strains in trans-infected lines that had previously been typed as single infections. This confirms the persistence of low-titer Wolbachia strains in microinjection experiments that had previously escaped standard detection techniques. Our study demonstrates that infections by multiple Wolbachia strains can shift in prevalence after artificial host transfer driven by either stochastic or selective processes. Trans-infection of Wolbachia can claim fitness costs in new hosts and we speculate that these costs may have driven the shifts of Wolbachia strains that we saw in our model system.Daniela I SchneiderMarkus RieglerWolfgang ArthoferHervé MerçotChristian StaufferWolfgang J MillerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82402 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela I Schneider Markus Riegler Wolfgang Arthofer Hervé Merçot Christian Stauffer Wolfgang J Miller Uncovering Wolbachia diversity upon artificial host transfer.
description	The common endosymbiotic Wolbachia bacteria influence arthropod hosts in multiple ways. They are mostly recognized for their manipulations of host reproduction, yet, more recent studies demonstrate that Wolbachia also impact host behavior, metabolic pathways and immunity. Besides their biological and evolutionary roles, Wolbachia are new potential biological control agents for pest and vector management. Importantly, Wolbachia-based control strategies require controlled symbiont transfer between host species and predictable outcomes of novel Wolbachia-host associations. Theoretically, this artificial horizontal transfer could inflict genetic changes within transferred Wolbachia populations. This could be facilitated through de novo mutations in the novel recipient host or changes of haplotype frequencies of polymorphic Wolbachia populations when transferred from donor to recipient hosts. Here we show that Wolbachia resident in the European cherry fruit fly, Rhagoletis cerasi, exhibit ancestral and cryptic sequence polymorphism in three symbiont genes, which are exposed upon microinjection into the new hosts Drosophila simulans and Ceratitis capitata. Our analyses of Wolbachia in microinjected D. simulans over 150 generations after microinjection uncovered infections with multiple Wolbachia strains in trans-infected lines that had previously been typed as single infections. This confirms the persistence of low-titer Wolbachia strains in microinjection experiments that had previously escaped standard detection techniques. Our study demonstrates that infections by multiple Wolbachia strains can shift in prevalence after artificial host transfer driven by either stochastic or selective processes. Trans-infection of Wolbachia can claim fitness costs in new hosts and we speculate that these costs may have driven the shifts of Wolbachia strains that we saw in our model system.
format	article
author	Daniela I Schneider Markus Riegler Wolfgang Arthofer Hervé Merçot Christian Stauffer Wolfgang J Miller
author_facet	Daniela I Schneider Markus Riegler Wolfgang Arthofer Hervé Merçot Christian Stauffer Wolfgang J Miller
author_sort	Daniela I Schneider
title	Uncovering Wolbachia diversity upon artificial host transfer.
title_short	Uncovering Wolbachia diversity upon artificial host transfer.
title_full	Uncovering Wolbachia diversity upon artificial host transfer.
title_fullStr	Uncovering Wolbachia diversity upon artificial host transfer.
title_full_unstemmed	Uncovering Wolbachia diversity upon artificial host transfer.
title_sort	uncovering wolbachia diversity upon artificial host transfer.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cdcdf97f3c754e958bfa4be5da63af85
work_keys_str_mv	AT danielaischneider uncoveringwolbachiadiversityuponartificialhosttransfer AT markusriegler uncoveringwolbachiadiversityuponartificialhosttransfer AT wolfgangarthofer uncoveringwolbachiadiversityuponartificialhosttransfer AT hervemercot uncoveringwolbachiadiversityuponartificialhosttransfer AT christianstauffer uncoveringwolbachiadiversityuponartificialhosttransfer AT wolfgangjmiller uncoveringwolbachiadiversityuponartificialhosttransfer
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Uncovering Wolbachia diversity upon artificial host transfer.

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