Colorimetric determination of urea using diacetyl monoxime with strong acids.

Urea is a byproduct of the urea cycle in metabolism and is excreted through urine and sweat. Ammonia, which is toxic at low levels, is converted to the safe storage form of urea, which represents the largest efflux of nitrogen from many organisms. Urea is an important nitrogen source in agriculture,...

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Autores principales:	Noah James Langenfeld, Lauren Elizabeth Payne, Bruce Bugbee
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdcecfeade83450db4137df3779778c2
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spelling	oai:doaj.org-article:cdcecfeade83450db4137df3779778c22021-12-02T20:05:56ZColorimetric determination of urea using diacetyl monoxime with strong acids.1932-620310.1371/journal.pone.0259760https://doaj.org/article/cdcecfeade83450db4137df3779778c22021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259760https://doaj.org/toc/1932-6203Urea is a byproduct of the urea cycle in metabolism and is excreted through urine and sweat. Ammonia, which is toxic at low levels, is converted to the safe storage form of urea, which represents the largest efflux of nitrogen from many organisms. Urea is an important nitrogen source in agriculture, is added to many industrial products, and is a large component in wastewater. The enzyme urease hydrolyzes urea to ammonia and bicarbonate. This reaction is microbially mediated in soils, hydroponic solutions, and wastewater recycling and is catalyzed in vivo in plants using native urease, making measurement of urea environmentally important. Both direct and indirect methods to measure urea exist. This protocol uses diacetyl monoxime to directly determine the concentration of urea in solution. The protocol provides repeatable results and stable reagents with good color stability and simple measurement techniques for use in any lab with a spectrophotometer. The reaction between diacetyl monoxime and urea in the presence of sulfuric acid, phosphoric acid, thiosemicarbazide, and ferric chloride produces a chromophore with a peak absorbance at 520 nm and a linear relationship between concentration and absorbance from 0.4 to 5.0 mM urea in this protocol. The lack of detectable interferences makes this protocol suitable for the determination of millimolar levels of urea in wastewater streams and hydroponic solutions.Noah James LangenfeldLauren Elizabeth PayneBruce BugbeePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259760 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Noah James Langenfeld Lauren Elizabeth Payne Bruce Bugbee Colorimetric determination of urea using diacetyl monoxime with strong acids.
description	Urea is a byproduct of the urea cycle in metabolism and is excreted through urine and sweat. Ammonia, which is toxic at low levels, is converted to the safe storage form of urea, which represents the largest efflux of nitrogen from many organisms. Urea is an important nitrogen source in agriculture, is added to many industrial products, and is a large component in wastewater. The enzyme urease hydrolyzes urea to ammonia and bicarbonate. This reaction is microbially mediated in soils, hydroponic solutions, and wastewater recycling and is catalyzed in vivo in plants using native urease, making measurement of urea environmentally important. Both direct and indirect methods to measure urea exist. This protocol uses diacetyl monoxime to directly determine the concentration of urea in solution. The protocol provides repeatable results and stable reagents with good color stability and simple measurement techniques for use in any lab with a spectrophotometer. The reaction between diacetyl monoxime and urea in the presence of sulfuric acid, phosphoric acid, thiosemicarbazide, and ferric chloride produces a chromophore with a peak absorbance at 520 nm and a linear relationship between concentration and absorbance from 0.4 to 5.0 mM urea in this protocol. The lack of detectable interferences makes this protocol suitable for the determination of millimolar levels of urea in wastewater streams and hydroponic solutions.
format	article
author	Noah James Langenfeld Lauren Elizabeth Payne Bruce Bugbee
author_facet	Noah James Langenfeld Lauren Elizabeth Payne Bruce Bugbee
author_sort	Noah James Langenfeld
title	Colorimetric determination of urea using diacetyl monoxime with strong acids.
title_short	Colorimetric determination of urea using diacetyl monoxime with strong acids.
title_full	Colorimetric determination of urea using diacetyl monoxime with strong acids.
title_fullStr	Colorimetric determination of urea using diacetyl monoxime with strong acids.
title_full_unstemmed	Colorimetric determination of urea using diacetyl monoxime with strong acids.
title_sort	colorimetric determination of urea using diacetyl monoxime with strong acids.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/cdcecfeade83450db4137df3779778c2
work_keys_str_mv	AT noahjameslangenfeld colorimetricdeterminationofureausingdiacetylmonoximewithstrongacids AT laurenelizabethpayne colorimetricdeterminationofureausingdiacetylmonoximewithstrongacids AT brucebugbee colorimetricdeterminationofureausingdiacetylmonoximewithstrongacids
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Colorimetric determination of urea using diacetyl monoxime with strong acids.

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