Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks

Abstract Background The oral administration of polystyrene-microplastics (PS-MPs) causes chronic constipation of ICR mice, but there are no reports on their effects on the inflammatory response in the colon. To determine if the oral administration of MPs causes inflammation in the colon, the changes...

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Autores principales:	Yun Ju Choi, Ji Eun Kim, Su Jin Lee, Jeong Eun Gong, You Jeong Jin, Sungbaek Seo, Jae Ho Lee, Dae Youn Hwang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Microplastics Inflammation Colon NF-κB Cytokines Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cdd2a194dd4f4c4082db5179869fbc4e
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spelling	oai:doaj.org-article:cdd2a194dd4f4c4082db5179869fbc4e2021-11-28T12:14:29ZInflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks10.1186/s42826-021-00109-w2233-7660https://doaj.org/article/cdd2a194dd4f4c4082db5179869fbc4e2021-11-01T00:00:00Zhttps://doi.org/10.1186/s42826-021-00109-whttps://doaj.org/toc/2233-7660Abstract Background The oral administration of polystyrene-microplastics (PS-MPs) causes chronic constipation of ICR mice, but there are no reports on their effects on the inflammatory response in the colon. To determine if the oral administration of MPs causes inflammation in the colon, the changes in the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC)-inflammasome pathway, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway, and inflammatory cytokine expression were evaluated in the mid colon of ICR mice treated with 0.5 μm size PS-MPs for two weeks. Results The thicknesses of the mucosa, muscle, flat luminal surface, and crypt layer were decreased significantly (p < 0.01) in the mid colon of the MPs treated group compared to the Vehicle treated group. On the other hand, a remarkable increase in the expression levels of NOD-like receptor pyrin domain-containing protein (NLRP) 3, ASC, and Cleaved Caspase (Cas)-1 protein was observed in the MPs treated group. In addition, similar increasing pattern in the levels of p-NF-κB and phospho-inhibitory subunit of NF-κB (p-IkB) α protein was detected. Four inflammatory cytokines, including NF-κB, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β, showed an increased expression level after the MPs treatment. Conclusions Therefore, the present study suggests that PS-MPs can be a novel cause of an inflammatory response in the mid colon of ICR mice.Yun Ju ChoiJi Eun KimSu Jin LeeJeong Eun GongYou Jeong JinSungbaek SeoJae Ho LeeDae Youn HwangBMCarticleMicroplasticsInflammationColonNF-κBCytokinesMedicine (General)R5-920Biology (General)QH301-705.5ENLaboratory Animal Research, Vol 37, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microplastics Inflammation Colon NF-κB Cytokines Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Microplastics Inflammation Colon NF-κB Cytokines Medicine (General) R5-920 Biology (General) QH301-705.5 Yun Ju Choi Ji Eun Kim Su Jin Lee Jeong Eun Gong You Jeong Jin Sungbaek Seo Jae Ho Lee Dae Youn Hwang Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks
description	Abstract Background The oral administration of polystyrene-microplastics (PS-MPs) causes chronic constipation of ICR mice, but there are no reports on their effects on the inflammatory response in the colon. To determine if the oral administration of MPs causes inflammation in the colon, the changes in the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC)-inflammasome pathway, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway, and inflammatory cytokine expression were evaluated in the mid colon of ICR mice treated with 0.5 μm size PS-MPs for two weeks. Results The thicknesses of the mucosa, muscle, flat luminal surface, and crypt layer were decreased significantly (p < 0.01) in the mid colon of the MPs treated group compared to the Vehicle treated group. On the other hand, a remarkable increase in the expression levels of NOD-like receptor pyrin domain-containing protein (NLRP) 3, ASC, and Cleaved Caspase (Cas)-1 protein was observed in the MPs treated group. In addition, similar increasing pattern in the levels of p-NF-κB and phospho-inhibitory subunit of NF-κB (p-IkB) α protein was detected. Four inflammatory cytokines, including NF-κB, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β, showed an increased expression level after the MPs treatment. Conclusions Therefore, the present study suggests that PS-MPs can be a novel cause of an inflammatory response in the mid colon of ICR mice.
format	article
author	Yun Ju Choi Ji Eun Kim Su Jin Lee Jeong Eun Gong You Jeong Jin Sungbaek Seo Jae Ho Lee Dae Youn Hwang
author_facet	Yun Ju Choi Ji Eun Kim Su Jin Lee Jeong Eun Gong You Jeong Jin Sungbaek Seo Jae Ho Lee Dae Youn Hwang
author_sort	Yun Ju Choi
title	Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks
title_short	Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks
title_full	Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks
title_fullStr	Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks
title_full_unstemmed	Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks
title_sort	inflammatory response in the mid colon of icr mice treated with polystyrene microplastics for two weeks
publisher	BMC
publishDate	2021
url	https://doaj.org/article/cdd2a194dd4f4c4082db5179869fbc4e
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Inflammatory response in the mid colon of ICR mice treated with polystyrene microplastics for two weeks

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