Natural killer cells induce eosinophil activation and apoptosis.

Eosinophils are potent inflammatory cells with numerous immune functions, including antigen presentation and exacerbation of inflammatory responses through their capacity to release a range of largely preformed cytokines and lipid mediators. Thus, timely regulation of eosinophil activation and apopt...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ali Awad, Hanane Yassine, Mathieu Barrier, Han Vorng, Philippe Marquillies, Anne Tsicopoulos, Catherine Duez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdd312ab8a364469b6aa4c25e9ddc9c7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cdd312ab8a364469b6aa4c25e9ddc9c7
record_format	dspace
spelling	oai:doaj.org-article:cdd312ab8a364469b6aa4c25e9ddc9c72021-11-18T08:23:46ZNatural killer cells induce eosinophil activation and apoptosis.1932-620310.1371/journal.pone.0094492https://doaj.org/article/cdd312ab8a364469b6aa4c25e9ddc9c72014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24727794/?tool=EBIhttps://doaj.org/toc/1932-6203Eosinophils are potent inflammatory cells with numerous immune functions, including antigen presentation and exacerbation of inflammatory responses through their capacity to release a range of largely preformed cytokines and lipid mediators. Thus, timely regulation of eosinophil activation and apoptosis is crucial to develop beneficial immune response and to avoid tissue damage and induce resolution of inflammation. Natural Killer (NK) cells have been reported to influence innate and adaptive immune responses by multiple mechanisms including cytotoxicity against other immune cells. In this study, we analyzed the effect of the interaction between NK cells and eosinophils. Co-culture experiments revealed that human NK cells could trigger autologous eosinophil activation, as shown by up-regulation of CD69 and down-regulation of CD62L, as well as degranulation, evidenced by increased CD63 surface expression, secretion of eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN). Moreover, NK cells significantly and dose dependently increased eosinophil apoptosis as shown by annexin V and propidium iodide (PI) staining. Direct contact was necessary for eosinophil degranulation and apoptosis. Increased expression of phosphorylated extracellular signal-regulated kinase (ERK) in cocultured eosinophils and inhibition of eosinophil CD63 expression by pharmacologic inhibitors suggest that MAPK and PI3K pathways are involved in NK cell-induced eosinophil degranulation. Finally, we showed that NK cells increased reactive oxygen species (ROS) expression by eosinophils in co-culture and that mitochondrial inhibitors (rotenone and antimycin) partially diminished NK cell-induced eosinophil apoptosis, suggesting the implication of mitochondrial ROS in NK cell-induced eosinophil apoptosis. Pan-caspase inhibitor (ZVAD-FMK) only slightly decreased eosinophil apoptosis in coculture. Altogether, our results suggest that NK cells regulate eosinophil functions by inducing their activation and their apoptosis.Ali AwadHanane YassineMathieu BarrierHan VorngPhilippe MarquilliesAnne TsicopoulosCatherine DuezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e94492 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ali Awad Hanane Yassine Mathieu Barrier Han Vorng Philippe Marquillies Anne Tsicopoulos Catherine Duez Natural killer cells induce eosinophil activation and apoptosis.
description	Eosinophils are potent inflammatory cells with numerous immune functions, including antigen presentation and exacerbation of inflammatory responses through their capacity to release a range of largely preformed cytokines and lipid mediators. Thus, timely regulation of eosinophil activation and apoptosis is crucial to develop beneficial immune response and to avoid tissue damage and induce resolution of inflammation. Natural Killer (NK) cells have been reported to influence innate and adaptive immune responses by multiple mechanisms including cytotoxicity against other immune cells. In this study, we analyzed the effect of the interaction between NK cells and eosinophils. Co-culture experiments revealed that human NK cells could trigger autologous eosinophil activation, as shown by up-regulation of CD69 and down-regulation of CD62L, as well as degranulation, evidenced by increased CD63 surface expression, secretion of eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN). Moreover, NK cells significantly and dose dependently increased eosinophil apoptosis as shown by annexin V and propidium iodide (PI) staining. Direct contact was necessary for eosinophil degranulation and apoptosis. Increased expression of phosphorylated extracellular signal-regulated kinase (ERK) in cocultured eosinophils and inhibition of eosinophil CD63 expression by pharmacologic inhibitors suggest that MAPK and PI3K pathways are involved in NK cell-induced eosinophil degranulation. Finally, we showed that NK cells increased reactive oxygen species (ROS) expression by eosinophils in co-culture and that mitochondrial inhibitors (rotenone and antimycin) partially diminished NK cell-induced eosinophil apoptosis, suggesting the implication of mitochondrial ROS in NK cell-induced eosinophil apoptosis. Pan-caspase inhibitor (ZVAD-FMK) only slightly decreased eosinophil apoptosis in coculture. Altogether, our results suggest that NK cells regulate eosinophil functions by inducing their activation and their apoptosis.
format	article
author	Ali Awad Hanane Yassine Mathieu Barrier Han Vorng Philippe Marquillies Anne Tsicopoulos Catherine Duez
author_facet	Ali Awad Hanane Yassine Mathieu Barrier Han Vorng Philippe Marquillies Anne Tsicopoulos Catherine Duez
author_sort	Ali Awad
title	Natural killer cells induce eosinophil activation and apoptosis.
title_short	Natural killer cells induce eosinophil activation and apoptosis.
title_full	Natural killer cells induce eosinophil activation and apoptosis.
title_fullStr	Natural killer cells induce eosinophil activation and apoptosis.
title_full_unstemmed	Natural killer cells induce eosinophil activation and apoptosis.
title_sort	natural killer cells induce eosinophil activation and apoptosis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/cdd312ab8a364469b6aa4c25e9ddc9c7
work_keys_str_mv	AT aliawad naturalkillercellsinduceeosinophilactivationandapoptosis AT hananeyassine naturalkillercellsinduceeosinophilactivationandapoptosis AT mathieubarrier naturalkillercellsinduceeosinophilactivationandapoptosis AT hanvorng naturalkillercellsinduceeosinophilactivationandapoptosis AT philippemarquillies naturalkillercellsinduceeosinophilactivationandapoptosis AT annetsicopoulos naturalkillercellsinduceeosinophilactivationandapoptosis AT catherineduez naturalkillercellsinduceeosinophilactivationandapoptosis
_version_	1718421890358312960

Natural killer cells induce eosinophil activation and apoptosis.

Ejemplares similares