The cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.

Clostridium perfringens is the causative agent of clostridial myonecrosis or gas gangrene and produces many different extracellular toxins and enzymes, including the cysteine protease α-clostripain. Mutation of the α-clostripain structural gene, ccp, alters the turnover of secreted extracellular pro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anjana Chakravorty, Milena M Awad, Thomas J Hiscox, Jackie K Cheung, Glen P Carter, Jocelyn M Choo, Dena Lyras, Julian I Rood
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdd50de2fdbb4a7f839702ab1e424f54
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cdd50de2fdbb4a7f839702ab1e424f54
record_format	dspace
spelling	oai:doaj.org-article:cdd50de2fdbb4a7f839702ab1e424f542021-11-18T06:49:02ZThe cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.1932-620310.1371/journal.pone.0022762https://doaj.org/article/cdd50de2fdbb4a7f839702ab1e424f542011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21829506/?tool=EBIhttps://doaj.org/toc/1932-6203Clostridium perfringens is the causative agent of clostridial myonecrosis or gas gangrene and produces many different extracellular toxins and enzymes, including the cysteine protease α-clostripain. Mutation of the α-clostripain structural gene, ccp, alters the turnover of secreted extracellular proteins in C. perfringens, but the role of α-clostripain in disease pathogenesis is not known. We insertionally inactivated the ccp gene C. perfringens strain 13 using TargeTron technology, constructing a strain that was no longer proteolytic on skim milk agar. Quantitative protease assays confirmed the absence of extracellular protease activity, which was restored by complementation with the wild-type ccp gene. The role of α-clostripain in virulence was assessed by analysing the isogenic wild-type, mutant and complemented strains in a mouse myonecrosis model. The results showed that although α-clostripain was the major extracellular protease, mutation of the ccp gene did not alter either the progression or the development of disease. These results do not rule out the possibility that this extracellular enzyme may still have a role in the early stages of the disease process.Anjana ChakravortyMilena M AwadThomas J HiscoxJackie K CheungGlen P CarterJocelyn M ChooDena LyrasJulian I RoodPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22762 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anjana Chakravorty Milena M Awad Thomas J Hiscox Jackie K Cheung Glen P Carter Jocelyn M Choo Dena Lyras Julian I Rood The cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.
description	Clostridium perfringens is the causative agent of clostridial myonecrosis or gas gangrene and produces many different extracellular toxins and enzymes, including the cysteine protease α-clostripain. Mutation of the α-clostripain structural gene, ccp, alters the turnover of secreted extracellular proteins in C. perfringens, but the role of α-clostripain in disease pathogenesis is not known. We insertionally inactivated the ccp gene C. perfringens strain 13 using TargeTron technology, constructing a strain that was no longer proteolytic on skim milk agar. Quantitative protease assays confirmed the absence of extracellular protease activity, which was restored by complementation with the wild-type ccp gene. The role of α-clostripain in virulence was assessed by analysing the isogenic wild-type, mutant and complemented strains in a mouse myonecrosis model. The results showed that although α-clostripain was the major extracellular protease, mutation of the ccp gene did not alter either the progression or the development of disease. These results do not rule out the possibility that this extracellular enzyme may still have a role in the early stages of the disease process.
format	article
author	Anjana Chakravorty Milena M Awad Thomas J Hiscox Jackie K Cheung Glen P Carter Jocelyn M Choo Dena Lyras Julian I Rood
author_facet	Anjana Chakravorty Milena M Awad Thomas J Hiscox Jackie K Cheung Glen P Carter Jocelyn M Choo Dena Lyras Julian I Rood
author_sort	Anjana Chakravorty
title	The cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.
title_short	The cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.
title_full	The cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.
title_fullStr	The cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.
title_full_unstemmed	The cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.
title_sort	cysteine protease α-clostripain is not essential for the pathogenesis of clostridium perfringens-mediated myonecrosis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/cdd50de2fdbb4a7f839702ab1e424f54
work_keys_str_mv	AT anjanachakravorty thecysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT milenamawad thecysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT thomasjhiscox thecysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT jackiekcheung thecysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT glenpcarter thecysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT jocelynmchoo thecysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT denalyras thecysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT julianirood thecysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT anjanachakravorty cysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT milenamawad cysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT thomasjhiscox cysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT jackiekcheung cysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT glenpcarter cysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT jocelynmchoo cysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT denalyras cysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis AT julianirood cysteineproteaseaclostripainisnotessentialforthepathogenesisofclostridiumperfringensmediatedmyonecrosis
_version_	1718424380068855808

The cysteine protease α-clostripain is not essential for the pathogenesis of Clostridium perfringens-mediated myonecrosis.

Ejemplares similares