Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study

Abstract Decline in episodic memory performance usually causes the first clinical symptoms of Alzheimer’s disease. At present, Alzheimer’s disease can only be diagnosed at a very late stage when neurodegeneration and cognitive impairment is already irreversible. New early disease markers are needed...

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Autores principales:	M. Konki, N. Lindgren, M. Kyläniemi, R. Venho, E. Laajala, B. Ghimire, R. Lahesmaa, J. Kaprio, J. O. Rinne, R. J. Lund
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdd6a220711b46849621446b2d220093
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spelling	oai:doaj.org-article:cdd6a220711b46849621446b2d2200932021-12-02T19:02:37ZPlasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study10.1038/s41598-020-71239-92045-2322https://doaj.org/article/cdd6a220711b46849621446b2d2200932020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-71239-9https://doaj.org/toc/2045-2322Abstract Decline in episodic memory performance usually causes the first clinical symptoms of Alzheimer’s disease. At present, Alzheimer’s disease can only be diagnosed at a very late stage when neurodegeneration and cognitive impairment is already irreversible. New early disease markers are needed for earlier and more efficient Alzheimer’s disease intervention. To identify early disease markers, we implemented a genome-wide bisulphite sequencing method for the analysis of plasma cell-free DNA methylation profiles and compared differences associated with episodic memory performance in Finnish twin pairs. A noticeable amount of cell-free DNA was present in plasma, however, the amounts as well as the genomic coverage of these fragments varied substantially between individuals. We found no significant markers associated with episodic memory performance in the twins’ plasma cell-free DNA methylation profiles. Furthermore, our results indicate that due to the low genomic coverage of cell-free DNA fragments and the variety in these fragments between individuals, the implemented genome-wide bisulphite sequencing method is not optimal for comparing cell-free DNA methylation differences between large groups of individuals.M. KonkiN. LindgrenM. KyläniemiR. VenhoE. LaajalaB. GhimireR. LahesmaaJ. KaprioJ. O. RinneR. J. LundNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Konki N. Lindgren M. Kyläniemi R. Venho E. Laajala B. Ghimire R. Lahesmaa J. Kaprio J. O. Rinne R. J. Lund Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study
description	Abstract Decline in episodic memory performance usually causes the first clinical symptoms of Alzheimer’s disease. At present, Alzheimer’s disease can only be diagnosed at a very late stage when neurodegeneration and cognitive impairment is already irreversible. New early disease markers are needed for earlier and more efficient Alzheimer’s disease intervention. To identify early disease markers, we implemented a genome-wide bisulphite sequencing method for the analysis of plasma cell-free DNA methylation profiles and compared differences associated with episodic memory performance in Finnish twin pairs. A noticeable amount of cell-free DNA was present in plasma, however, the amounts as well as the genomic coverage of these fragments varied substantially between individuals. We found no significant markers associated with episodic memory performance in the twins’ plasma cell-free DNA methylation profiles. Furthermore, our results indicate that due to the low genomic coverage of cell-free DNA fragments and the variety in these fragments between individuals, the implemented genome-wide bisulphite sequencing method is not optimal for comparing cell-free DNA methylation differences between large groups of individuals.
format	article
author	M. Konki N. Lindgren M. Kyläniemi R. Venho E. Laajala B. Ghimire R. Lahesmaa J. Kaprio J. O. Rinne R. J. Lund
author_facet	M. Konki N. Lindgren M. Kyläniemi R. Venho E. Laajala B. Ghimire R. Lahesmaa J. Kaprio J. O. Rinne R. J. Lund
author_sort	M. Konki
title	Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study
title_short	Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study
title_full	Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study
title_fullStr	Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study
title_full_unstemmed	Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study
title_sort	plasma cell-free dna methylation marks for episodic memory impairment: a pilot twin study
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/cdd6a220711b46849621446b2d220093
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Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study

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