Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences

Priyadi DA, Panjono, Bintara S, Hartatik T. 2017. The genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences. Biodiversitas 18: 795-800. The IGFBP-3 gene is a potential marker of cattle growth, which is related to the functi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	DWI AHMAD PRIYADI, PANJONO PANJONO, SIGIT BINTARA, TETY HARTATIK
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	brahman cattle brahman cross cattle genetic marker insulin-like growth factor binding protein-3 (igfbp-3) single nucleotide polymorphism (snp) Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cdd7a20dbc7c4043bb27472553dad4b5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cdd7a20dbc7c4043bb27472553dad4b5
record_format	dspace
spelling	oai:doaj.org-article:cdd7a20dbc7c4043bb27472553dad4b52021-11-16T13:52:30ZGenotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences1412-033X2085-472210.13057/biodiv/d180255https://doaj.org/article/cdd7a20dbc7c4043bb27472553dad4b52017-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/1714https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Priyadi DA, Panjono, Bintara S, Hartatik T. 2017. The genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences. Biodiversitas 18: 795-800. The IGFBP-3 gene is a potential marker of cattle growth, which is related to the functions that influence the growth, energy metabolism, reproduction and immunity. The genome was isolated from whole blood samples of 10 Brahman cattle and 16 Brahman Cross cattle. Cattle IGFBP-3 gene that targeted in this study was located in the part of intron 2, exon 3 and part of intron 3. The gene targets were amplified using specific primers by Polymerase Chain Reaction (PCR) technique, resulting 563 bp amplification product. As data comparison to reveal the SNP, the GenBank sequences (n = 14) from various breed and countries were used. The objective of this study was to reveal the SNP on the Brahman and Brahman Cross IGFBP-3 gene. The results obtained 3 genotypes from one SNP that spread in the sample population. The SNP was located in intron 2 at position 3,930 (Gïƒ A). The Polymorphism could be recognized by PvuII restriction enzymes. There was not enough evidence to associate the SNP with the phenotype (at pre-weaning age) of Brahman and Brahman Cross cattle. There was a genetic diversity in the population studied. Knowledgeable SNP could be used as genetic markers for further research on Brahman and Brahman Cross cattle.DWI AHMAD PRIYADIPANJONO PANJONOSIGIT BINTARATETY HARTATIKMBI & UNS Soloarticlebrahman cattlebrahman cross cattlegenetic markerinsulin-like growth factor binding protein-3 (igfbp-3)single nucleotide polymorphism (snp)Biology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 2, Pp 795-800 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	brahman cattle brahman cross cattle genetic marker insulin-like growth factor binding protein-3 (igfbp-3) single nucleotide polymorphism (snp) Biology (General) QH301-705.5
spellingShingle	brahman cattle brahman cross cattle genetic marker insulin-like growth factor binding protein-3 (igfbp-3) single nucleotide polymorphism (snp) Biology (General) QH301-705.5 DWI AHMAD PRIYADI PANJONO PANJONO SIGIT BINTARA TETY HARTATIK Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences
description	Priyadi DA, Panjono, Bintara S, Hartatik T. 2017. The genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences. Biodiversitas 18: 795-800. The IGFBP-3 gene is a potential marker of cattle growth, which is related to the functions that influence the growth, energy metabolism, reproduction and immunity. The genome was isolated from whole blood samples of 10 Brahman cattle and 16 Brahman Cross cattle. Cattle IGFBP-3 gene that targeted in this study was located in the part of intron 2, exon 3 and part of intron 3. The gene targets were amplified using specific primers by Polymerase Chain Reaction (PCR) technique, resulting 563 bp amplification product. As data comparison to reveal the SNP, the GenBank sequences (n = 14) from various breed and countries were used. The objective of this study was to reveal the SNP on the Brahman and Brahman Cross IGFBP-3 gene. The results obtained 3 genotypes from one SNP that spread in the sample population. The SNP was located in intron 2 at position 3,930 (Gïƒ A). The Polymorphism could be recognized by PvuII restriction enzymes. There was not enough evidence to associate the SNP with the phenotype (at pre-weaning age) of Brahman and Brahman Cross cattle. There was a genetic diversity in the population studied. Knowledgeable SNP could be used as genetic markers for further research on Brahman and Brahman Cross cattle.
format	article
author	DWI AHMAD PRIYADI PANJONO PANJONO SIGIT BINTARA TETY HARTATIK
author_facet	DWI AHMAD PRIYADI PANJONO PANJONO SIGIT BINTARA TETY HARTATIK
author_sort	DWI AHMAD PRIYADI
title	Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences
title_short	Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences
title_full	Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences
title_fullStr	Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences
title_full_unstemmed	Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences
title_sort	genotype of brahman and brahman cross cattle based on snp in insulin-like growth factor binding protein-3 (igfbp-3) gene sequences
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/cdd7a20dbc7c4043bb27472553dad4b5
work_keys_str_mv	AT dwiahmadpriyadi genotypeofbrahmanandbrahmancrosscattlebasedonsnpininsulinlikegrowthfactorbindingprotein3igfbp3genesequences AT panjonopanjono genotypeofbrahmanandbrahmancrosscattlebasedonsnpininsulinlikegrowthfactorbindingprotein3igfbp3genesequences AT sigitbintara genotypeofbrahmanandbrahmancrosscattlebasedonsnpininsulinlikegrowthfactorbindingprotein3igfbp3genesequences AT tetyhartatik genotypeofbrahmanandbrahmancrosscattlebasedonsnpininsulinlikegrowthfactorbindingprotein3igfbp3genesequences
_version_	1718426507921063936

Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences

Ejemplares similares