Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia

Abstract. Fachrial E, Anggraini S, Harmileni, Nugroho TT, Saryono. 2019. Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia. Biodiversitas 20: 3493-3498. The application of enzymes indus...

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Autores principales:	Edy Fachrial, Sari Anggraini, Harmileni, Titania T Nugroho, Saryono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	carbohydrase, hot spring, protease, thermophilic bacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cdd86ccfcf38448c9d0a4d12a43345c4
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spelling	oai:doaj.org-article:cdd86ccfcf38448c9d0a4d12a43345c42021-11-21T21:59:06ZIsolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia1412-033X2085-472210.13057/biodiv/d201205https://doaj.org/article/cdd86ccfcf38448c9d0a4d12a43345c42019-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4543https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Fachrial E, Anggraini S, Harmileni, Nugroho TT, Saryono. 2019. Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia. Biodiversitas 20: 3493-3498. The application of enzymes industrially is increasing every year and thermophilic microbes are a promising source of these enzymes for industrial use due to their temperature stability. The aim of this study, therefore, was to isolate, characterize and identify the enzyme producer-thermophilic bacteria from the Penen Hot Spring in Deli Serdang, North Sumatra, Indonesia. In the experimental setup, carbohydrase activities including inulinase and amylase were determined by the formation of clear zone around the colonies after soaking with Lugol on the TSA medium supplemented with 1% inulin powder and 1% starch. Similarly, the protease activities were determined by the formation of clear zone around the colonies on Skim Milk Agar Medium. Then, of the 11 isolates, only one known as UTMP 12 showed maximum enzyme activity. The isolate was then characterized based on morphology and biochemistry and found to be Bacillus subtilis strain JCM 1465 (accession number NR_113265, homolog 99.72%). Furthermore, this is the first study on carbohydrase and protease activities of Bacillus subtilis strain JCM 1465 and the result shows that the thermophilic bacteria are needed in the production of carbohydrase and protease.Edy FachrialSari AnggrainiHarmileniTitania T NugrohoSaryonoMBI & UNS Soloarticlecarbohydrase, hot spring, protease, thermophilic bacteriaBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbohydrase, hot spring, protease, thermophilic bacteria Biology (General) QH301-705.5
spellingShingle	carbohydrase, hot spring, protease, thermophilic bacteria Biology (General) QH301-705.5 Edy Fachrial Sari Anggraini Harmileni Titania T Nugroho Saryono Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia
description	Abstract. Fachrial E, Anggraini S, Harmileni, Nugroho TT, Saryono. 2019. Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia. Biodiversitas 20: 3493-3498. The application of enzymes industrially is increasing every year and thermophilic microbes are a promising source of these enzymes for industrial use due to their temperature stability. The aim of this study, therefore, was to isolate, characterize and identify the enzyme producer-thermophilic bacteria from the Penen Hot Spring in Deli Serdang, North Sumatra, Indonesia. In the experimental setup, carbohydrase activities including inulinase and amylase were determined by the formation of clear zone around the colonies after soaking with Lugol on the TSA medium supplemented with 1% inulin powder and 1% starch. Similarly, the protease activities were determined by the formation of clear zone around the colonies on Skim Milk Agar Medium. Then, of the 11 isolates, only one known as UTMP 12 showed maximum enzyme activity. The isolate was then characterized based on morphology and biochemistry and found to be Bacillus subtilis strain JCM 1465 (accession number NR_113265, homolog 99.72%). Furthermore, this is the first study on carbohydrase and protease activities of Bacillus subtilis strain JCM 1465 and the result shows that the thermophilic bacteria are needed in the production of carbohydrase and protease.
format	article
author	Edy Fachrial Sari Anggraini Harmileni Titania T Nugroho Saryono
author_facet	Edy Fachrial Sari Anggraini Harmileni Titania T Nugroho Saryono
author_sort	Edy Fachrial
title	Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia
title_short	Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia
title_full	Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia
title_fullStr	Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia
title_full_unstemmed	Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia
title_sort	isolation and molecular identification of carbohydrase and protease producing bacillus subtilis jcm 1465 isolated from penen hot springs in north sumatra, indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/cdd86ccfcf38448c9d0a4d12a43345c4
work_keys_str_mv	AT edyfachrial isolationandmolecularidentificationofcarbohydraseandproteaseproducingbacillussubtilisjcm1465isolatedfrompenenhotspringsinnorthsumatraindonesia AT sarianggraini isolationandmolecularidentificationofcarbohydraseandproteaseproducingbacillussubtilisjcm1465isolatedfrompenenhotspringsinnorthsumatraindonesia AT harmileni isolationandmolecularidentificationofcarbohydraseandproteaseproducingbacillussubtilisjcm1465isolatedfrompenenhotspringsinnorthsumatraindonesia AT titaniatnugroho isolationandmolecularidentificationofcarbohydraseandproteaseproducingbacillussubtilisjcm1465isolatedfrompenenhotspringsinnorthsumatraindonesia AT saryono isolationandmolecularidentificationofcarbohydraseandproteaseproducingbacillussubtilisjcm1465isolatedfrompenenhotspringsinnorthsumatraindonesia
_version_	1718418609247617024

Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia

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