A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria

Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of t...

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Autores principales:	Andrea Palombieri, Ilia Tsachev, Vittorio Sarchese, Paola Fruci, Federica Di Profio, Roman Pepovich, Magdalena Baymakova, Fulvio Marsilio, Vito Martella, Barbara Di Martino
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hepatitis E virus (HEV) RNA genotype 3 pigs Bulgaria Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/cddd4d1789f5494e896808d12b8b5034
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spelling	oai:doaj.org-article:cddd4d1789f5494e896808d12b8b50342021-11-25T19:12:00ZA Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria10.3390/vetsci81102672306-7381https://doaj.org/article/cddd4d1789f5494e896808d12b8b50342021-11-01T00:00:00Zhttps://www.mdpi.com/2306-7381/8/11/267https://doaj.org/toc/2306-7381Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of the partial polymerase (RdRp) region revealed the highest genetic correlation with HEVs of genotype (Gt) 3 identified in French and Dutch patients. For three such strains, a 700-bp fragment of the open reading frame 2 gene was generated. On phylogenetic analysis, the Bulgarian strains clustered tightly (93.8–98.3% nt) with human and animal HEVs classified within the Gt3 subtype c.Andrea PalombieriIlia TsachevVittorio SarchesePaola FruciFederica Di ProfioRoman PepovichMagdalena BaymakovaFulvio MarsilioVito MartellaBarbara Di MartinoMDPI AGarticlehepatitis E virus (HEV)RNAgenotype 3pigsBulgariaVeterinary medicineSF600-1100ENVeterinary Sciences, Vol 8, Iss 267, p 267 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis E virus (HEV) RNA genotype 3 pigs Bulgaria Veterinary medicine SF600-1100
spellingShingle	hepatitis E virus (HEV) RNA genotype 3 pigs Bulgaria Veterinary medicine SF600-1100 Andrea Palombieri Ilia Tsachev Vittorio Sarchese Paola Fruci Federica Di Profio Roman Pepovich Magdalena Baymakova Fulvio Marsilio Vito Martella Barbara Di Martino A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
description	Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of the partial polymerase (RdRp) region revealed the highest genetic correlation with HEVs of genotype (Gt) 3 identified in French and Dutch patients. For three such strains, a 700-bp fragment of the open reading frame 2 gene was generated. On phylogenetic analysis, the Bulgarian strains clustered tightly (93.8–98.3% nt) with human and animal HEVs classified within the Gt3 subtype c.
format	article
author	Andrea Palombieri Ilia Tsachev Vittorio Sarchese Paola Fruci Federica Di Profio Roman Pepovich Magdalena Baymakova Fulvio Marsilio Vito Martella Barbara Di Martino
author_facet	Andrea Palombieri Ilia Tsachev Vittorio Sarchese Paola Fruci Federica Di Profio Roman Pepovich Magdalena Baymakova Fulvio Marsilio Vito Martella Barbara Di Martino
author_sort	Andrea Palombieri
title	A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_short	A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_full	A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_fullStr	A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_full_unstemmed	A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_sort	molecular study on hepatitis e virus (hev) in pigs in bulgaria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cddd4d1789f5494e896808d12b8b5034
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A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria

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