Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart

Abstract Background Our previous study indicated that Potentilla reptans root has a preconditioning effect by its antioxidant and anti-apoptotic effects in an isolated rat heart ischemia/reperfusion (IR) model. In the present study, we investigated the post-conditioning cardio-protective effects of...

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Autores principales:	Ayesheh Enayati, Aref Salehi, Mostafa Alilou, Hermann Stuppner, Mirali Polshekan, Maryam Rajaei, Mona Pourabouk, Ali Jabbari, Zohreh Mazaheri, Narguess Yassa, Hamid Reza Moheimani, Vahid Khori
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Potentilla reptans root Postconditioning Anti-apoptotic Antioxidant NO GSK-3β Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/cde7c981b51e41d39ddae2dd084682f7
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spelling	oai:doaj.org-article:cde7c981b51e41d39ddae2dd084682f72021-11-28T12:37:36ZPotentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart10.1186/s12906-021-03456-22662-7671https://doaj.org/article/cde7c981b51e41d39ddae2dd084682f72021-11-01T00:00:00Zhttps://doi.org/10.1186/s12906-021-03456-2https://doaj.org/toc/2662-7671Abstract Background Our previous study indicated that Potentilla reptans root has a preconditioning effect by its antioxidant and anti-apoptotic effects in an isolated rat heart ischemia/reperfusion (IR) model. In the present study, we investigated the post-conditioning cardio-protective effects of Potentilla reptans and its active substances. Methods The ethyl acetate fraction of P. reptans root (Et) was subjected to an IR model under 30 min of ischemia and 100 min of reperfusion. To investigate the postconditioning effect, Et was perfused for 15 min at the early phase of reperfusion. RISK/SAFE pathway inhibitors, 5HD and L-NAME, were applied individually 10 min before the ischemia, either alone or in combination with Et during the early reperfusion phase. The hemodynamic factors and ventricular arrhythmia were calculated during the reperfusion. Oxidative stress, apoptosis markers, GSK-3β and SGK1 proteins were assessed at the end of experiments. Results Et postconditioning (Etpost) significantly reduced the infarct size, arrhythmia score, ventricular fibrillation incidence, and enhanced the hemodynamic parameters by decreasing the MDA level and increasing expression of Nrf2, SOD and CAT activities. Meanwhile, Etpost increased the BCl-2/BAX ratio and decreased Caspase-3 expression. The cardioprotective effect of Etpost was abrogated by L-NAME, Wortmannin (a PI3K/Akt inhibitor), and AG490 (a JAK/STAT3 inhibitor). Finally, Etpost reduced the expression of GSK-3β and SGK1 proteins pertaining to the IR group. Conclusion P. reptans reveals the post-conditioning effects via the Nrf2 pathway, NO release, and the RISK/SAFE pathway. Also, Etpost decreased apoptotic indexes by inhibiting GSK-3β and SGK1 expressions. Hence, our data suggest that Etpost can be a suitable natural candidate to protect cardiomyocytes during reperfusion injury.Ayesheh EnayatiAref SalehiMostafa AlilouHermann StuppnerMirali PolshekanMaryam RajaeiMona PouraboukAli JabbariZohreh MazaheriNarguess YassaHamid Reza MoheimaniVahid KhoriBMCarticlePotentilla reptans rootPostconditioningAnti-apoptoticAntioxidantNOGSK-3βOther systems of medicineRZ201-999ENBMC Complementary Medicine and Therapies, Vol 21, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Potentilla reptans root Postconditioning Anti-apoptotic Antioxidant NO GSK-3β Other systems of medicine RZ201-999
spellingShingle	Potentilla reptans root Postconditioning Anti-apoptotic Antioxidant NO GSK-3β Other systems of medicine RZ201-999 Ayesheh Enayati Aref Salehi Mostafa Alilou Hermann Stuppner Mirali Polshekan Maryam Rajaei Mona Pourabouk Ali Jabbari Zohreh Mazaheri Narguess Yassa Hamid Reza Moheimani Vahid Khori Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart
description	Abstract Background Our previous study indicated that Potentilla reptans root has a preconditioning effect by its antioxidant and anti-apoptotic effects in an isolated rat heart ischemia/reperfusion (IR) model. In the present study, we investigated the post-conditioning cardio-protective effects of Potentilla reptans and its active substances. Methods The ethyl acetate fraction of P. reptans root (Et) was subjected to an IR model under 30 min of ischemia and 100 min of reperfusion. To investigate the postconditioning effect, Et was perfused for 15 min at the early phase of reperfusion. RISK/SAFE pathway inhibitors, 5HD and L-NAME, were applied individually 10 min before the ischemia, either alone or in combination with Et during the early reperfusion phase. The hemodynamic factors and ventricular arrhythmia were calculated during the reperfusion. Oxidative stress, apoptosis markers, GSK-3β and SGK1 proteins were assessed at the end of experiments. Results Et postconditioning (Etpost) significantly reduced the infarct size, arrhythmia score, ventricular fibrillation incidence, and enhanced the hemodynamic parameters by decreasing the MDA level and increasing expression of Nrf2, SOD and CAT activities. Meanwhile, Etpost increased the BCl-2/BAX ratio and decreased Caspase-3 expression. The cardioprotective effect of Etpost was abrogated by L-NAME, Wortmannin (a PI3K/Akt inhibitor), and AG490 (a JAK/STAT3 inhibitor). Finally, Etpost reduced the expression of GSK-3β and SGK1 proteins pertaining to the IR group. Conclusion P. reptans reveals the post-conditioning effects via the Nrf2 pathway, NO release, and the RISK/SAFE pathway. Also, Etpost decreased apoptotic indexes by inhibiting GSK-3β and SGK1 expressions. Hence, our data suggest that Etpost can be a suitable natural candidate to protect cardiomyocytes during reperfusion injury.
format	article
author	Ayesheh Enayati Aref Salehi Mostafa Alilou Hermann Stuppner Mirali Polshekan Maryam Rajaei Mona Pourabouk Ali Jabbari Zohreh Mazaheri Narguess Yassa Hamid Reza Moheimani Vahid Khori
author_facet	Ayesheh Enayati Aref Salehi Mostafa Alilou Hermann Stuppner Mirali Polshekan Maryam Rajaei Mona Pourabouk Ali Jabbari Zohreh Mazaheri Narguess Yassa Hamid Reza Moheimani Vahid Khori
author_sort	Ayesheh Enayati
title	Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart
title_short	Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart
title_full	Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart
title_fullStr	Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart
title_full_unstemmed	Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart
title_sort	potentilla reptans l. postconditioning protects reperfusion injury via the risk/safe pathways in an isolated rat heart
publisher	BMC
publishDate	2021
url	https://doaj.org/article/cde7c981b51e41d39ddae2dd084682f7
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Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart

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