Data on Connexin 43 hemichannels regulation of cellular redox in lens

This article describes a dataset that is related to the research paper “Connexin hemichannels regulate redox potential via metabolite exchange and protect lens against cellular oxidative damage”. Growing evidence demonstrates that oxidative stress is a key event in cataract formation. Hemichannels (...

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Autores principales:	Yumeng Quan, Yu Du, Changrui Wu, Sumin Gu, Jean X. Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Connexin Hemichannel Redox metabolite Redox regulation Lens Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/cdea95d1b9af453aa859fa0d513fd49b
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spelling	oai:doaj.org-article:cdea95d1b9af453aa859fa0d513fd49b2021-11-18T04:49:20ZData on Connexin 43 hemichannels regulation of cellular redox in lens2352-340910.1016/j.dib.2021.107572https://doaj.org/article/cdea95d1b9af453aa859fa0d513fd49b2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352340921008477https://doaj.org/toc/2352-3409This article describes a dataset that is related to the research paper “Connexin hemichannels regulate redox potential via metabolite exchange and protect lens against cellular oxidative damage”. Growing evidence demonstrates that oxidative stress is a key event in cataract formation. Hemichannels (HCs) formed by Connexin (Cx) 43, a Cx subtype only present in the epithelium of lens tissue, mediate the exchange of small molecules between the intracellular and extracellular environments, including redox-related metabolic molecules, such as glutathione (GSH) and reactive oxygen species (ROS). Here, we used a Cx43 heterozygous mouse model, Cx43E2 antibody (a specific Cx43 HC blocker), and knocked down Cx43 expression by siRNA in human lens epithelial HLE-B3 cells to assess the oxidative response of Cx43 HCs to H2O2 and UVB radiation. Western blot analysis of heterozygous Cx43-null (Cx43+/−) mouse lenses showed the haploinsufficiency of Cx43 protein. We further assessed anti-oxidative gene expression in response to H2O2 and UVB radiation treatment in the Cx43-deficient lens epithelial cells. This dataset will be useful for understanding the critical role of Cx43 HCs in maintaining redox homeostasis in the lens under oxidative stress.Yumeng QuanYu DuChangrui WuSumin GuJean X. JiangElsevierarticleConnexinHemichannelRedox metaboliteRedox regulationLensComputer applications to medicine. Medical informaticsR858-859.7Science (General)Q1-390ENData in Brief, Vol 39, Iss , Pp 107572- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Connexin Hemichannel Redox metabolite Redox regulation Lens Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390
spellingShingle	Connexin Hemichannel Redox metabolite Redox regulation Lens Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390 Yumeng Quan Yu Du Changrui Wu Sumin Gu Jean X. Jiang Data on Connexin 43 hemichannels regulation of cellular redox in lens
description	This article describes a dataset that is related to the research paper “Connexin hemichannels regulate redox potential via metabolite exchange and protect lens against cellular oxidative damage”. Growing evidence demonstrates that oxidative stress is a key event in cataract formation. Hemichannels (HCs) formed by Connexin (Cx) 43, a Cx subtype only present in the epithelium of lens tissue, mediate the exchange of small molecules between the intracellular and extracellular environments, including redox-related metabolic molecules, such as glutathione (GSH) and reactive oxygen species (ROS). Here, we used a Cx43 heterozygous mouse model, Cx43E2 antibody (a specific Cx43 HC blocker), and knocked down Cx43 expression by siRNA in human lens epithelial HLE-B3 cells to assess the oxidative response of Cx43 HCs to H2O2 and UVB radiation. Western blot analysis of heterozygous Cx43-null (Cx43+/−) mouse lenses showed the haploinsufficiency of Cx43 protein. We further assessed anti-oxidative gene expression in response to H2O2 and UVB radiation treatment in the Cx43-deficient lens epithelial cells. This dataset will be useful for understanding the critical role of Cx43 HCs in maintaining redox homeostasis in the lens under oxidative stress.
format	article
author	Yumeng Quan Yu Du Changrui Wu Sumin Gu Jean X. Jiang
author_facet	Yumeng Quan Yu Du Changrui Wu Sumin Gu Jean X. Jiang
author_sort	Yumeng Quan
title	Data on Connexin 43 hemichannels regulation of cellular redox in lens
title_short	Data on Connexin 43 hemichannels regulation of cellular redox in lens
title_full	Data on Connexin 43 hemichannels regulation of cellular redox in lens
title_fullStr	Data on Connexin 43 hemichannels regulation of cellular redox in lens
title_full_unstemmed	Data on Connexin 43 hemichannels regulation of cellular redox in lens
title_sort	data on connexin 43 hemichannels regulation of cellular redox in lens
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/cdea95d1b9af453aa859fa0d513fd49b
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Data on Connexin 43 hemichannels regulation of cellular redox in lens

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