CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome

CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome

Abstract CRISPR/Cas9-based gene knockout in animal cells, particularly in teleosts, has proven to be very efficient with regards to mutation rates, but the precise insertion of exogenous DNA or gene knock-in via the homology-directed repair (HDR) pathway has seldom been achieved outside of the model...

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Autores principales: Rhoda Mae C. Simora, De Xing, Max R. Bangs, Wenwen Wang, Xiaoli Ma, Baofeng Su, Mohd G. Q. Khan, Zhenkui Qin, Cuiyu Lu, Veronica Alston, Darshika Hettiarachchi, Andrew Johnson, Shangjia Li, Michael Coogan, Jeremy Gurbatow, Jeffery S. Terhune, Xu Wang, Rex A. Dunham
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/cdedef54f68c49f1baf0d0bd4a6c8ded
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