Efficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.

Evolution of resistance in pests threatens the long-term efficacy of insecticidal proteins from Bacillus thuringiensis (Bt) used in sprays and transgenic crops. Previous work showed that genetically modified Bt toxins Cry1AbMod and Cry1AcMod effectively countered resistance to native Bt toxins Cry1A...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bruce E Tabashnik, Jeffrey A Fabrick, Gopalan C Unnithan, Alex J Yelich, Luke Masson, Jie Zhang, Alejandra Bravo, Mario Soberón
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdefc5d2f11b42d9b20291561aadc0e2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cdefc5d2f11b42d9b20291561aadc0e2
record_format	dspace
spelling	oai:doaj.org-article:cdefc5d2f11b42d9b20291561aadc0e22021-11-18T08:47:49ZEfficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.1932-620310.1371/journal.pone.0080496https://doaj.org/article/cdefc5d2f11b42d9b20291561aadc0e22013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24244692/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Evolution of resistance in pests threatens the long-term efficacy of insecticidal proteins from Bacillus thuringiensis (Bt) used in sprays and transgenic crops. Previous work showed that genetically modified Bt toxins Cry1AbMod and Cry1AcMod effectively countered resistance to native Bt toxins Cry1Ab and Cry1Ac in some pests, including pink bollworm (Pectinophora gossypiella). Here we report that Cry1AbMod and Cry1AcMod were also effective against a laboratory-selected strain of pink bollworm resistant to Cry2Ab as well as to Cry1Ab and Cry1Ac. Resistance ratios based on the concentration of toxin killing 50% of larvae for the resistant strain relative to a susceptible strain were 210 for Cry2Ab, 270 for Cry1Ab, and 310 for Cry1Ac, but only 1.6 for Cry1AbMod and 2.1 for Cry1AcMod. To evaluate the interactions among toxins, we tested combinations of Cry1AbMod, Cry1Ac, and Cry2Ab. For both the resistant and susceptible strains, the net results across all concentrations tested showed slight but significant synergism between Cry1AbMod and Cry2Ab, whereas the other combinations of toxins did not show consistent synergism or antagonism. The results suggest that the modified toxins might be useful for controlling populations of pink bollworm resistant to Cry1Ac, Cry2Ab, or both.Bruce E TabashnikJeffrey A FabrickGopalan C UnnithanAlex J YelichLuke MassonJie ZhangAlejandra BravoMario SoberónPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80496 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bruce E Tabashnik Jeffrey A Fabrick Gopalan C Unnithan Alex J Yelich Luke Masson Jie Zhang Alejandra Bravo Mario Soberón Efficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.
description	Evolution of resistance in pests threatens the long-term efficacy of insecticidal proteins from Bacillus thuringiensis (Bt) used in sprays and transgenic crops. Previous work showed that genetically modified Bt toxins Cry1AbMod and Cry1AcMod effectively countered resistance to native Bt toxins Cry1Ab and Cry1Ac in some pests, including pink bollworm (Pectinophora gossypiella). Here we report that Cry1AbMod and Cry1AcMod were also effective against a laboratory-selected strain of pink bollworm resistant to Cry2Ab as well as to Cry1Ab and Cry1Ac. Resistance ratios based on the concentration of toxin killing 50% of larvae for the resistant strain relative to a susceptible strain were 210 for Cry2Ab, 270 for Cry1Ab, and 310 for Cry1Ac, but only 1.6 for Cry1AbMod and 2.1 for Cry1AcMod. To evaluate the interactions among toxins, we tested combinations of Cry1AbMod, Cry1Ac, and Cry2Ab. For both the resistant and susceptible strains, the net results across all concentrations tested showed slight but significant synergism between Cry1AbMod and Cry2Ab, whereas the other combinations of toxins did not show consistent synergism or antagonism. The results suggest that the modified toxins might be useful for controlling populations of pink bollworm resistant to Cry1Ac, Cry2Ab, or both.
format	article
author	Bruce E Tabashnik Jeffrey A Fabrick Gopalan C Unnithan Alex J Yelich Luke Masson Jie Zhang Alejandra Bravo Mario Soberón
author_facet	Bruce E Tabashnik Jeffrey A Fabrick Gopalan C Unnithan Alex J Yelich Luke Masson Jie Zhang Alejandra Bravo Mario Soberón
author_sort	Bruce E Tabashnik
title	Efficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.
title_short	Efficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.
title_full	Efficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.
title_fullStr	Efficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.
title_full_unstemmed	Efficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.
title_sort	efficacy of genetically modified bt toxins alone and in combinations against pink bollworm resistant to cry1ac and cry2ab.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cdefc5d2f11b42d9b20291561aadc0e2
work_keys_str_mv	AT bruceetabashnik efficacyofgeneticallymodifiedbttoxinsaloneandincombinationsagainstpinkbollwormresistanttocry1acandcry2ab AT jeffreyafabrick efficacyofgeneticallymodifiedbttoxinsaloneandincombinationsagainstpinkbollwormresistanttocry1acandcry2ab AT gopalancunnithan efficacyofgeneticallymodifiedbttoxinsaloneandincombinationsagainstpinkbollwormresistanttocry1acandcry2ab AT alexjyelich efficacyofgeneticallymodifiedbttoxinsaloneandincombinationsagainstpinkbollwormresistanttocry1acandcry2ab AT lukemasson efficacyofgeneticallymodifiedbttoxinsaloneandincombinationsagainstpinkbollwormresistanttocry1acandcry2ab AT jiezhang efficacyofgeneticallymodifiedbttoxinsaloneandincombinationsagainstpinkbollwormresistanttocry1acandcry2ab AT alejandrabravo efficacyofgeneticallymodifiedbttoxinsaloneandincombinationsagainstpinkbollwormresistanttocry1acandcry2ab AT mariosoberon efficacyofgeneticallymodifiedbttoxinsaloneandincombinationsagainstpinkbollwormresistanttocry1acandcry2ab
_version_	1718421350905806848

Efficacy of genetically modified Bt toxins alone and in combinations against pink bollworm resistant to Cry1Ac and Cry2Ab.

Ejemplares similares