Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles

Abstract Exosome-like vesicles (ELV) are involved in mediating radiation-induced bystander effect (RIBE). Here, we used ELV from control cell conditioned medium (CCCM) and from 4 Gy of X-ray irradiated cell conditioned medium (ICCM), which has been used to culture normal human fibroblast cells to ex...

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Autores principales:	Kentaro Ariyoshi, Tomisato Miura, Kosuke Kasai, Yohei Fujishima, Akifumi Nakata, Mitsuaki Yoshida
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdf02e44df7a4c7ba0ffedca4052b086
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spelling	oai:doaj.org-article:cdf02e44df7a4c7ba0ffedca4052b0862021-12-02T15:09:39ZRadiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles10.1038/s41598-019-45669-z2045-2322https://doaj.org/article/cdf02e44df7a4c7ba0ffedca4052b0862019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45669-zhttps://doaj.org/toc/2045-2322Abstract Exosome-like vesicles (ELV) are involved in mediating radiation-induced bystander effect (RIBE). Here, we used ELV from control cell conditioned medium (CCCM) and from 4 Gy of X-ray irradiated cell conditioned medium (ICCM), which has been used to culture normal human fibroblast cells to examine the possibility of ELV mediating RIBE signals. We investigated whether ELV from 4 Gy irradiated mouse serum mediate RIBE signals. Induction of DNA damage was observed in cells that were treated with ICCM ELV and ELV from 4 Gy irradiated mouse serum. In addition, we treated CCCM ELV and ICCM ELV with RNases, DNases, and proteinases to determine which component of ELV is responsible for RIBE. Induction of DNA damage by ICCM ELV was not observed after treatment with DNases. After treatment, DNA damages were not induced in CCCM ELV or ICCM ELV from mitochondria depleted (ρ0) normal human fibroblast cells. Further, we found significant increase in mitochondrial DNA (mtDNA) in ICCM ELV and ELV from 4 Gy irradiated mouse serum. ELV carrying amplified mtDNA (ND1, ND5) induced DNA damage in treated cells. These data suggest that the secretion of mtDNA through exosomes is involved in mediating RIBE signals.Kentaro AriyoshiTomisato MiuraKosuke KasaiYohei FujishimaAkifumi NakataMitsuaki YoshidaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kentaro Ariyoshi Tomisato Miura Kosuke Kasai Yohei Fujishima Akifumi Nakata Mitsuaki Yoshida Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles
description	Abstract Exosome-like vesicles (ELV) are involved in mediating radiation-induced bystander effect (RIBE). Here, we used ELV from control cell conditioned medium (CCCM) and from 4 Gy of X-ray irradiated cell conditioned medium (ICCM), which has been used to culture normal human fibroblast cells to examine the possibility of ELV mediating RIBE signals. We investigated whether ELV from 4 Gy irradiated mouse serum mediate RIBE signals. Induction of DNA damage was observed in cells that were treated with ICCM ELV and ELV from 4 Gy irradiated mouse serum. In addition, we treated CCCM ELV and ICCM ELV with RNases, DNases, and proteinases to determine which component of ELV is responsible for RIBE. Induction of DNA damage by ICCM ELV was not observed after treatment with DNases. After treatment, DNA damages were not induced in CCCM ELV or ICCM ELV from mitochondria depleted (ρ0) normal human fibroblast cells. Further, we found significant increase in mitochondrial DNA (mtDNA) in ICCM ELV and ELV from 4 Gy irradiated mouse serum. ELV carrying amplified mtDNA (ND1, ND5) induced DNA damage in treated cells. These data suggest that the secretion of mtDNA through exosomes is involved in mediating RIBE signals.
format	article
author	Kentaro Ariyoshi Tomisato Miura Kosuke Kasai Yohei Fujishima Akifumi Nakata Mitsuaki Yoshida
author_facet	Kentaro Ariyoshi Tomisato Miura Kosuke Kasai Yohei Fujishima Akifumi Nakata Mitsuaki Yoshida
author_sort	Kentaro Ariyoshi
title	Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles
title_short	Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles
title_full	Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles
title_fullStr	Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles
title_full_unstemmed	Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles
title_sort	radiation-induced bystander effect is mediated by mitochondrial dna in exosome-like vesicles
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cdf02e44df7a4c7ba0ffedca4052b086
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Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles

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