Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)

The aim of this work was to investigate the in vitro cytotoxic effect of previously developed nanocapsules, nanoemulsion, and microemulsion based on bullfrog oil (BFO) against human melanoma cells (A2058). The nanosystems were produced as described in previous studies and characterized according to...

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Autores principales:	W.N. Oliveira, E.N. Alencar, H.A.O. Rocha, L. Amaral-Machado, E.S.T. Egito
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Rana catesbeiana Shaw Nanocapsules Nanoemulsion Microemulsion Anti-melanoma activity Cell necrosis Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/cdf0505df85148fa87ebfea6d6538540
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spelling	oai:doaj.org-article:cdf0505df85148fa87ebfea6d65385402021-12-02T04:59:06ZNanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)0753-332210.1016/j.biopha.2021.112438https://doaj.org/article/cdf0505df85148fa87ebfea6d65385402022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012245https://doaj.org/toc/0753-3322The aim of this work was to investigate the in vitro cytotoxic effect of previously developed nanocapsules, nanoemulsion, and microemulsion based on bullfrog oil (BFO) against human melanoma cells (A2058). The nanosystems were produced as described in previous studies and characterized according to droplet/particle distribution and zeta potential. The biocompatibility was evaluated by the determination of the hemolytic potential against human erythrocytes. The cytotoxicity assessment was based on MTT and cell death assays, determination of Reactive Oxygen Species (ROS) levels, and cell uptake. The nanosystems were successfully reproduced and showed hemolytic potential smaller than 10% at all oil concentrations (50 and 100 µg.mL−1) (p < 0.05). The MTT assay revealed that the nanosystems decreased the mitochondrial activity up to 92 ± 2% (p < 0.05). The study showed that the free BFO induced cell apoptosis, while all the nanostructured systems caused cell death by necrosis associated with a ROS overproduction. This can be related to the increased ability of the nanostructured systems to deliver the BFO across all cellular compartments (membrane, cytoplasm, and nucleus). Finally, these results elucidate the in vitro BFO nanosystems cytotoxic effect against human melanoma cells (A2058), revealing the emulsified ones as the most cytotoxic systems. Overall, the findings suggest that the safety and antineoplastic activity of these systems can be further investigated by in vivo studies.W.N. OliveiraE.N. AlencarH.A.O. RochaL. Amaral-MachadoE.S.T. EgitoElsevierarticleRana catesbeiana ShawNanocapsulesNanoemulsionMicroemulsionAnti-melanoma activityCell necrosisTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112438- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rana catesbeiana Shaw Nanocapsules Nanoemulsion Microemulsion Anti-melanoma activity Cell necrosis Therapeutics. Pharmacology RM1-950
spellingShingle	Rana catesbeiana Shaw Nanocapsules Nanoemulsion Microemulsion Anti-melanoma activity Cell necrosis Therapeutics. Pharmacology RM1-950 W.N. Oliveira E.N. Alencar H.A.O. Rocha L. Amaral-Machado E.S.T. Egito Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)
description	The aim of this work was to investigate the in vitro cytotoxic effect of previously developed nanocapsules, nanoemulsion, and microemulsion based on bullfrog oil (BFO) against human melanoma cells (A2058). The nanosystems were produced as described in previous studies and characterized according to droplet/particle distribution and zeta potential. The biocompatibility was evaluated by the determination of the hemolytic potential against human erythrocytes. The cytotoxicity assessment was based on MTT and cell death assays, determination of Reactive Oxygen Species (ROS) levels, and cell uptake. The nanosystems were successfully reproduced and showed hemolytic potential smaller than 10% at all oil concentrations (50 and 100 µg.mL−1) (p < 0.05). The MTT assay revealed that the nanosystems decreased the mitochondrial activity up to 92 ± 2% (p < 0.05). The study showed that the free BFO induced cell apoptosis, while all the nanostructured systems caused cell death by necrosis associated with a ROS overproduction. This can be related to the increased ability of the nanostructured systems to deliver the BFO across all cellular compartments (membrane, cytoplasm, and nucleus). Finally, these results elucidate the in vitro BFO nanosystems cytotoxic effect against human melanoma cells (A2058), revealing the emulsified ones as the most cytotoxic systems. Overall, the findings suggest that the safety and antineoplastic activity of these systems can be further investigated by in vivo studies.
format	article
author	W.N. Oliveira E.N. Alencar H.A.O. Rocha L. Amaral-Machado E.S.T. Egito
author_facet	W.N. Oliveira E.N. Alencar H.A.O. Rocha L. Amaral-Machado E.S.T. Egito
author_sort	W.N. Oliveira
title	Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)
title_short	Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)
title_full	Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)
title_fullStr	Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)
title_full_unstemmed	Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)
title_sort	nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (a2058)
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/cdf0505df85148fa87ebfea6d6538540
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Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058)

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