Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo

Abstract The low ratio of embryonic callus (EC) induction has inhibited the rapid development of maize genetic engineering. Still, little is known to explain the genotype-dependence of EC induction. Here, we performed a large-scale, quantitative analysis of the maize EC metabolome and proteome at th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fei Ge, Hongmei Hu, Xing Huang, Yanling Zhang, Yanli Wang, Zhaoling Li, Chaoying Zou, Huanwei Peng, Lujiang Li, Shibin Gao, Guangtang Pan, Yaou Shen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdf24eeae9cc48cc927511a428b7e9d0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cdf24eeae9cc48cc927511a428b7e9d0
record_format	dspace
spelling	oai:doaj.org-article:cdf24eeae9cc48cc927511a428b7e9d02021-12-02T15:04:51ZMetabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo10.1038/s41598-017-01280-82045-2322https://doaj.org/article/cdf24eeae9cc48cc927511a428b7e9d02017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01280-8https://doaj.org/toc/2045-2322Abstract The low ratio of embryonic callus (EC) induction has inhibited the rapid development of maize genetic engineering. Still, little is known to explain the genotype-dependence of EC induction. Here, we performed a large-scale, quantitative analysis of the maize EC metabolome and proteome at three typical induction stages in two inbred lines with a range of EC induction capabilities. Comparison of the metabolomes and proteomes suggests that the differential molecular responses begin at an early stage of development and continue throughout the process of EC formation. The two inbred lines show different responses under various conditions, such as metal ion binding, cell enlargement, stem cell formation, meristematic activity maintenance, somatic embryogenesis, cell wall synthesis, and hormone signal transduction. Furthermore, the differences in hormone (auxin, cytokinin, gibberellin, salicylic acid, jasmonic acid, brassinosteroid and ethylene) synthesis and transduction ability could partially explain the higher EC induction ratio in the inbred line 18-599R. During EC formation, repression of the “histone deacetylase 2 and ERF transcription factors” complex in 18-599R activated the expression of downstream genes, which further promoted EC induction. Together, our data provide new insights into the molecular regulatory mechanism responsible for efficient EC induction in maize.Fei GeHongmei HuXing HuangYanling ZhangYanli WangZhaoling LiChaoying ZouHuanwei PengLujiang LiShibin GaoGuangtang PanYaou ShenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fei Ge Hongmei Hu Xing Huang Yanling Zhang Yanli Wang Zhaoling Li Chaoying Zou Huanwei Peng Lujiang Li Shibin Gao Guangtang Pan Yaou Shen Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo
description	Abstract The low ratio of embryonic callus (EC) induction has inhibited the rapid development of maize genetic engineering. Still, little is known to explain the genotype-dependence of EC induction. Here, we performed a large-scale, quantitative analysis of the maize EC metabolome and proteome at three typical induction stages in two inbred lines with a range of EC induction capabilities. Comparison of the metabolomes and proteomes suggests that the differential molecular responses begin at an early stage of development and continue throughout the process of EC formation. The two inbred lines show different responses under various conditions, such as metal ion binding, cell enlargement, stem cell formation, meristematic activity maintenance, somatic embryogenesis, cell wall synthesis, and hormone signal transduction. Furthermore, the differences in hormone (auxin, cytokinin, gibberellin, salicylic acid, jasmonic acid, brassinosteroid and ethylene) synthesis and transduction ability could partially explain the higher EC induction ratio in the inbred line 18-599R. During EC formation, repression of the “histone deacetylase 2 and ERF transcription factors” complex in 18-599R activated the expression of downstream genes, which further promoted EC induction. Together, our data provide new insights into the molecular regulatory mechanism responsible for efficient EC induction in maize.
format	article
author	Fei Ge Hongmei Hu Xing Huang Yanling Zhang Yanli Wang Zhaoling Li Chaoying Zou Huanwei Peng Lujiang Li Shibin Gao Guangtang Pan Yaou Shen
author_facet	Fei Ge Hongmei Hu Xing Huang Yanling Zhang Yanli Wang Zhaoling Li Chaoying Zou Huanwei Peng Lujiang Li Shibin Gao Guangtang Pan Yaou Shen
author_sort	Fei Ge
title	Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo
title_short	Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo
title_full	Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo
title_fullStr	Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo
title_full_unstemmed	Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo
title_sort	metabolomic and proteomic analysis of maize embryonic callus induced from immature embryo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cdf24eeae9cc48cc927511a428b7e9d0
work_keys_str_mv	AT feige metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT hongmeihu metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT xinghuang metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT yanlingzhang metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT yanliwang metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT zhaolingli metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT chaoyingzou metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT huanweipeng metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT lujiangli metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT shibingao metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT guangtangpan metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo AT yaoushen metabolomicandproteomicanalysisofmaizeembryoniccallusinducedfromimmatureembryo
_version_	1718389045255471104

Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo

Ejemplares similares