Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces

Abstract We report a straightforward and reproducible electrochemical approach to develop polydopamine-ethanolamine (ePDA-ETA) films to be used as immunosensing interfaces. ETA is strongly attached to polydopamine films during the potentiodynamic electropolymerization of dopamine. The great advantag...

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Autores principales:	Luís C. Almeida, Tânia Frade, Rui D. Correia, Yu Niu, Gang Jin, Jorge P. Correia, Ana S. Viana
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdf74a974bf94f06b3650336d8556e34
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spelling	oai:doaj.org-article:cdf74a974bf94f06b3650336d8556e342021-12-02T13:57:48ZElectrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces10.1038/s41598-021-81816-12045-2322https://doaj.org/article/cdf74a974bf94f06b3650336d8556e342021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81816-1https://doaj.org/toc/2045-2322Abstract We report a straightforward and reproducible electrochemical approach to develop polydopamine-ethanolamine (ePDA-ETA) films to be used as immunosensing interfaces. ETA is strongly attached to polydopamine films during the potentiodynamic electropolymerization of dopamine. The great advantage of the electrochemical methods is to generate the oxidized species (quinones), which can readily react with ETA amine groups present in solution, with the subsequent incorporation of this molecule in the polymer. The presence of ETA and its effect on the electrosynthesis of polydopamine was accessed by cyclic voltammetry, ellipsometry, atomic force microscopy, FTIR and X-ray photoelectron spectroscopy. The adhesive and biocompatible films enable a facile protein linkage, are resilient to flow assays, and display intrinsic anti-fouling properties to block non-specific protein interactions, as monitored by real-time surface plasmon resonance, and confirmed by ellipsometry. Immunoglobulin G (IgG) and Anti-IgG were used in this work as model proteins for the affinity sensor. By using the one-step methodology (ePDA-ETA), the lower amount of immobilized biorecognition element, IgG, compared to that deposited on ePDA or on ETA post-modified film (ePDA/ETA), allied to the presence of ETA, improved the antibody-antigen affinity interaction. The great potential of the developed platform is its versatility to be used with any target biorecognition molecules, allowing both optical and electrochemical detection.Luís C. AlmeidaTânia FradeRui D. CorreiaYu NiuGang JinJorge P. CorreiaAna S. VianaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luís C. Almeida Tânia Frade Rui D. Correia Yu Niu Gang Jin Jorge P. Correia Ana S. Viana Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces
description	Abstract We report a straightforward and reproducible electrochemical approach to develop polydopamine-ethanolamine (ePDA-ETA) films to be used as immunosensing interfaces. ETA is strongly attached to polydopamine films during the potentiodynamic electropolymerization of dopamine. The great advantage of the electrochemical methods is to generate the oxidized species (quinones), which can readily react with ETA amine groups present in solution, with the subsequent incorporation of this molecule in the polymer. The presence of ETA and its effect on the electrosynthesis of polydopamine was accessed by cyclic voltammetry, ellipsometry, atomic force microscopy, FTIR and X-ray photoelectron spectroscopy. The adhesive and biocompatible films enable a facile protein linkage, are resilient to flow assays, and display intrinsic anti-fouling properties to block non-specific protein interactions, as monitored by real-time surface plasmon resonance, and confirmed by ellipsometry. Immunoglobulin G (IgG) and Anti-IgG were used in this work as model proteins for the affinity sensor. By using the one-step methodology (ePDA-ETA), the lower amount of immobilized biorecognition element, IgG, compared to that deposited on ePDA or on ETA post-modified film (ePDA/ETA), allied to the presence of ETA, improved the antibody-antigen affinity interaction. The great potential of the developed platform is its versatility to be used with any target biorecognition molecules, allowing both optical and electrochemical detection.
format	article
author	Luís C. Almeida Tânia Frade Rui D. Correia Yu Niu Gang Jin Jorge P. Correia Ana S. Viana
author_facet	Luís C. Almeida Tânia Frade Rui D. Correia Yu Niu Gang Jin Jorge P. Correia Ana S. Viana
author_sort	Luís C. Almeida
title	Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces
title_short	Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces
title_full	Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces
title_fullStr	Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces
title_full_unstemmed	Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces
title_sort	electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cdf74a974bf94f06b3650336d8556e34
work_keys_str_mv	AT luiscalmeida electrosynthesisofpolydopamineethanolaminefilmsforthedevelopmentofimmunosensinginterfaces AT taniafrade electrosynthesisofpolydopamineethanolaminefilmsforthedevelopmentofimmunosensinginterfaces AT ruidcorreia electrosynthesisofpolydopamineethanolaminefilmsforthedevelopmentofimmunosensinginterfaces AT yuniu electrosynthesisofpolydopamineethanolaminefilmsforthedevelopmentofimmunosensinginterfaces AT gangjin electrosynthesisofpolydopamineethanolaminefilmsforthedevelopmentofimmunosensinginterfaces AT jorgepcorreia electrosynthesisofpolydopamineethanolaminefilmsforthedevelopmentofimmunosensinginterfaces AT anasviana electrosynthesisofpolydopamineethanolaminefilmsforthedevelopmentofimmunosensinginterfaces
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Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces

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