Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress

Abstract In different plant species, aquaporins (AQPs) facilitate water movement by regulating root hydraulic conductivity under diverse stress conditions such as salt and water stresses. To improve survival and yield of crop plants, a detailed understanding of stress responses is imperative and req...

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Autores principales:	Da-yong Zhang, Manoj Kumar, Ling Xu, Qun Wan, Yi-hong Huang, Zhao-Long Xu, Xiao-Lan He, Jin-Biao Ma, Girdhar K. Pandey, Hong-Bo Shao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdfb51f1ff774e17820cc9545bd6d592
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spelling	oai:doaj.org-article:cdfb51f1ff774e17820cc9545bd6d5922021-12-02T16:06:28ZGenome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress10.1038/s41598-017-04253-z2045-2322https://doaj.org/article/cdfb51f1ff774e17820cc9545bd6d5922017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04253-zhttps://doaj.org/toc/2045-2322Abstract In different plant species, aquaporins (AQPs) facilitate water movement by regulating root hydraulic conductivity under diverse stress conditions such as salt and water stresses. To improve survival and yield of crop plants, a detailed understanding of stress responses is imperative and required. We used Glycine soja genome as a tool to study AQPs, considering it shows abundant genetic diversity and higher salt environment tolerance features and identified 62 Gs AQP genes. Additionally, this study identifies major aquaporins responsive to salt and drought stresses in soybean and elucidates their mode of action through yeast two-hybrid assay and BiFC. Under stress condition, the expression analysis of AQPs in roots and leaves of two contrasting ecotypes of soybean revealed diverse expression patterns suggesting complex regulation at transcriptional level. Based on expression analysis, we identify GmTIP2;1 as a potential candidate involved in salinity and drought responses. The overexpression of GmTIP2;1 in Saccharomyces cerevisiae as well as in-planta enhanced salt and drought tolerance. We identified that GmTIP2;1 forms homodimers as well as interacts with GmTIP1;7 and GmTIP1;8. This study augments our knowledge of stress responsive pathways and also establishes GmTIP2;1 as a new stress responsive gene in imparting salt stress tolerance in soybean.Da-yong ZhangManoj KumarLing XuQun WanYi-hong HuangZhao-Long XuXiao-Lan HeJin-Biao MaGirdhar K. PandeyHong-Bo ShaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Da-yong Zhang Manoj Kumar Ling Xu Qun Wan Yi-hong Huang Zhao-Long Xu Xiao-Lan He Jin-Biao Ma Girdhar K. Pandey Hong-Bo Shao Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress
description	Abstract In different plant species, aquaporins (AQPs) facilitate water movement by regulating root hydraulic conductivity under diverse stress conditions such as salt and water stresses. To improve survival and yield of crop plants, a detailed understanding of stress responses is imperative and required. We used Glycine soja genome as a tool to study AQPs, considering it shows abundant genetic diversity and higher salt environment tolerance features and identified 62 Gs AQP genes. Additionally, this study identifies major aquaporins responsive to salt and drought stresses in soybean and elucidates their mode of action through yeast two-hybrid assay and BiFC. Under stress condition, the expression analysis of AQPs in roots and leaves of two contrasting ecotypes of soybean revealed diverse expression patterns suggesting complex regulation at transcriptional level. Based on expression analysis, we identify GmTIP2;1 as a potential candidate involved in salinity and drought responses. The overexpression of GmTIP2;1 in Saccharomyces cerevisiae as well as in-planta enhanced salt and drought tolerance. We identified that GmTIP2;1 forms homodimers as well as interacts with GmTIP1;7 and GmTIP1;8. This study augments our knowledge of stress responsive pathways and also establishes GmTIP2;1 as a new stress responsive gene in imparting salt stress tolerance in soybean.
format	article
author	Da-yong Zhang Manoj Kumar Ling Xu Qun Wan Yi-hong Huang Zhao-Long Xu Xiao-Lan He Jin-Biao Ma Girdhar K. Pandey Hong-Bo Shao
author_facet	Da-yong Zhang Manoj Kumar Ling Xu Qun Wan Yi-hong Huang Zhao-Long Xu Xiao-Lan He Jin-Biao Ma Girdhar K. Pandey Hong-Bo Shao
author_sort	Da-yong Zhang
title	Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress
title_short	Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress
title_full	Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress
title_fullStr	Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress
title_full_unstemmed	Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress
title_sort	genome-wide identification of major intrinsic proteins in glycine soja and characterization of gmtip2;1 function under salt and water stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cdfb51f1ff774e17820cc9545bd6d592
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Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress

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