The changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.

Many cartilage tissue engineering approaches aim to differentiate human mesenchymal stem cells (hMSCs) into chondrocytes and develop cartilage in vitro by targeting cell-matrix interactions. We sought to better inform the design of cartilage tissue engineering scaffolds by understanding how integrin...

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Autores principales:	Vanessa L S LaPointe, Amanda Verpoorte, Molly M Stevens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cdfffaf3ea7b4395be52832a01802e15
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spelling	oai:doaj.org-article:cdfffaf3ea7b4395be52832a01802e152021-11-18T08:44:21ZThe changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.1932-620310.1371/journal.pone.0082035https://doaj.org/article/cdfffaf3ea7b4395be52832a01802e152013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312400/?tool=EBIhttps://doaj.org/toc/1932-6203Many cartilage tissue engineering approaches aim to differentiate human mesenchymal stem cells (hMSCs) into chondrocytes and develop cartilage in vitro by targeting cell-matrix interactions. We sought to better inform the design of cartilage tissue engineering scaffolds by understanding how integrin expression changes during chondrogenic differentiation. In three models of in vitro chondrogenesis, we studied the temporal change of cartilage phenotype markers and integrin subunits during the differentiation of hMSCs. We found that transcript expression of most subunits was conserved across the chondrogenesis models, but was significantly affected by the time-course of differentiation. In particular, ITGB8 was up-regulated and its importance in chondrogenesis was further established by a knockdown of integrin β8, which resulted in a non-hyaline cartilage phenotype, with no COL2A1 expression detected. In conclusion, we performed a systematic study of the temporal changes of integrin expression during chondrogenic differentiation in multiple chondrogenesis models, and revealed a role for integrin β8 in chondrogenesis. This work enhances our understanding of the changing adhesion requirements of hMSCs during chondrogenic differentiation and underlines the importance of integrins in establishing a cartilage phenotype.Vanessa L S LaPointeAmanda VerpoorteMolly M StevensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e82035 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vanessa L S LaPointe Amanda Verpoorte Molly M Stevens The changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.
description	Many cartilage tissue engineering approaches aim to differentiate human mesenchymal stem cells (hMSCs) into chondrocytes and develop cartilage in vitro by targeting cell-matrix interactions. We sought to better inform the design of cartilage tissue engineering scaffolds by understanding how integrin expression changes during chondrogenic differentiation. In three models of in vitro chondrogenesis, we studied the temporal change of cartilage phenotype markers and integrin subunits during the differentiation of hMSCs. We found that transcript expression of most subunits was conserved across the chondrogenesis models, but was significantly affected by the time-course of differentiation. In particular, ITGB8 was up-regulated and its importance in chondrogenesis was further established by a knockdown of integrin β8, which resulted in a non-hyaline cartilage phenotype, with no COL2A1 expression detected. In conclusion, we performed a systematic study of the temporal changes of integrin expression during chondrogenic differentiation in multiple chondrogenesis models, and revealed a role for integrin β8 in chondrogenesis. This work enhances our understanding of the changing adhesion requirements of hMSCs during chondrogenic differentiation and underlines the importance of integrins in establishing a cartilage phenotype.
format	article
author	Vanessa L S LaPointe Amanda Verpoorte Molly M Stevens
author_facet	Vanessa L S LaPointe Amanda Verpoorte Molly M Stevens
author_sort	Vanessa L S LaPointe
title	The changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.
title_short	The changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.
title_full	The changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.
title_fullStr	The changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.
title_full_unstemmed	The changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.
title_sort	changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cdfffaf3ea7b4395be52832a01802e15
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The changing integrin expression and a role for integrin β8 in the chondrogenic differentiation of mesenchymal stem cells.

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