Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.

Phospholipid membranes are thought to be one of the main inducers of hemozoin formation in Plasmodia and other blood-feeding parasites. The "membrane surrounding hemozoin" has been observed in infected cells but has not been observed in in vitro experiments. This study focused on observing...

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Autores principales:	Nguyen Tien Huy, Yusuke Shima, Atsushi Maeda, Tran Thanh Men, Kenji Hirayama, Ai Hirase, Atsuo Miyazawa, Kaeko Kamei
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce02c8ba0c6e4a6282c57e7b0f3b2ee0
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spelling	oai:doaj.org-article:ce02c8ba0c6e4a6282c57e7b0f3b2ee02021-11-18T09:03:30ZPhospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.1932-620310.1371/journal.pone.0070025https://doaj.org/article/ce02c8ba0c6e4a6282c57e7b0f3b2ee02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23894579/?tool=EBIhttps://doaj.org/toc/1932-6203Phospholipid membranes are thought to be one of the main inducers of hemozoin formation in Plasmodia and other blood-feeding parasites. The "membrane surrounding hemozoin" has been observed in infected cells but has not been observed in in vitro experiments. This study focused on observing the association of phospholipid membranes and synthetic β-hematin, which is chemically identical to hemozoin, and on a further exploration into the mechanism of phospholipid membrane-induced β-hematin formation. Our results showed that β-hematin formation was induced by phospholipids in the fluid phase but not in the gel phase. The ability of phospholipids to induce β-hematin formation was inversely correlated with gel-to-liquid phase transition temperatures, suggesting an essential insertion of heme into the hydrocarbon chains of the phospholipid membrane to form β-hematin. For this study, a cryogenic transmission electron microscope was used to achieve the first direct observation of the formation of a monolayer of phospholipid membrane surrounding β-hematin.Nguyen Tien HuyYusuke ShimaAtsushi MaedaTran Thanh MenKenji HirayamaAi HiraseAtsuo MiyazawaKaeko KameiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e70025 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nguyen Tien Huy Yusuke Shima Atsushi Maeda Tran Thanh Men Kenji Hirayama Ai Hirase Atsuo Miyazawa Kaeko Kamei Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.
description	Phospholipid membranes are thought to be one of the main inducers of hemozoin formation in Plasmodia and other blood-feeding parasites. The "membrane surrounding hemozoin" has been observed in infected cells but has not been observed in in vitro experiments. This study focused on observing the association of phospholipid membranes and synthetic β-hematin, which is chemically identical to hemozoin, and on a further exploration into the mechanism of phospholipid membrane-induced β-hematin formation. Our results showed that β-hematin formation was induced by phospholipids in the fluid phase but not in the gel phase. The ability of phospholipids to induce β-hematin formation was inversely correlated with gel-to-liquid phase transition temperatures, suggesting an essential insertion of heme into the hydrocarbon chains of the phospholipid membrane to form β-hematin. For this study, a cryogenic transmission electron microscope was used to achieve the first direct observation of the formation of a monolayer of phospholipid membrane surrounding β-hematin.
format	article
author	Nguyen Tien Huy Yusuke Shima Atsushi Maeda Tran Thanh Men Kenji Hirayama Ai Hirase Atsuo Miyazawa Kaeko Kamei
author_facet	Nguyen Tien Huy Yusuke Shima Atsushi Maeda Tran Thanh Men Kenji Hirayama Ai Hirase Atsuo Miyazawa Kaeko Kamei
author_sort	Nguyen Tien Huy
title	Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.
title_short	Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.
title_full	Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.
title_fullStr	Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.
title_full_unstemmed	Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.
title_sort	phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ce02c8ba0c6e4a6282c57e7b0f3b2ee0
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Phospholipid membrane-mediated hemozoin formation: the effects of physical properties and evidence of membrane surrounding hemozoin.

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