Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.

<h4>Background</h4>Periodontal diseases are inflammatory processes that occur following the influx of neutrophils into the periodontal tissues in response to the subgingival bacterial biofilm. Current literature suggests that while neutrophils are protective and prevent bacterial infecti...

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Autores principales:	Flavia S Lakschevitz, Guy M Aboodi, Michael Glogauer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce04de63426a427fa7baa5bd5a5266fb
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spelling	oai:doaj.org-article:ce04de63426a427fa7baa5bd5a5266fb2021-11-18T07:37:47ZOral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.1932-620310.1371/journal.pone.0068983https://doaj.org/article/ce04de63426a427fa7baa5bd5a5266fb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874838/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Periodontal diseases are inflammatory processes that occur following the influx of neutrophils into the periodontal tissues in response to the subgingival bacterial biofilm. Current literature suggests that while neutrophils are protective and prevent bacterial infections, they also appear to contribute to damage of the periodontal tissues. In the present study we compare the gene expression profile changes in neutrophils as they migrate from the circulation into the oral tissues in patients with chronic periodontits and matched healthy subjects. We hypothesized that oral neutrophils in periodontal disease patients will display a disease specific transcriptome that differs from the oral neutrophil of healthy subjects.<h4>Methods</h4>Venous blood and oral rinse samples were obtained from healthy subjects and chronic periodontitis patients for neutrophil isolation. mRNA was isolated from the neutrophils, and gene expression microarray analysis was completed. Results were confirmed for specific genes of interest by qRT-PCR and Western Blot analysis.<h4>Results and discussion</h4>Chronic periodontitis patients presented with increased recruitment of neutrophils to the oral cavity. Gene expression analysis revealed differences in the expression levels of genes from several biological pathways. Using hierarchical clustering analysis, we found that the apoptosis network was significantly altered in patients with chronic inflammation in the oral cavity, with up-regulation of pro-survival members of the Bcl-2 family and down-regulation of pro-apoptosis members in the same compartment. Additional functional analysis confirmed that the percentages of viable neutrophils are significantly increased in the oral cavity of chronic periodontitis patients.<h4>Conclusions</h4>Oral neutrophils from patients with periodontal disease displayed an altered transcriptome following migration into the oral tissues. This resulted in a pro-survival neutrophil phenotype in chronic periodontitis patients when compared with healthy subjects, resulting in a longer-lived neutrophil. This is likely to impact the severity and length of the inflammatory response in this oral disease.Flavia S LakschevitzGuy M AboodiMichael GlogauerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68983 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Flavia S Lakschevitz Guy M Aboodi Michael Glogauer Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.
description	<h4>Background</h4>Periodontal diseases are inflammatory processes that occur following the influx of neutrophils into the periodontal tissues in response to the subgingival bacterial biofilm. Current literature suggests that while neutrophils are protective and prevent bacterial infections, they also appear to contribute to damage of the periodontal tissues. In the present study we compare the gene expression profile changes in neutrophils as they migrate from the circulation into the oral tissues in patients with chronic periodontits and matched healthy subjects. We hypothesized that oral neutrophils in periodontal disease patients will display a disease specific transcriptome that differs from the oral neutrophil of healthy subjects.<h4>Methods</h4>Venous blood and oral rinse samples were obtained from healthy subjects and chronic periodontitis patients for neutrophil isolation. mRNA was isolated from the neutrophils, and gene expression microarray analysis was completed. Results were confirmed for specific genes of interest by qRT-PCR and Western Blot analysis.<h4>Results and discussion</h4>Chronic periodontitis patients presented with increased recruitment of neutrophils to the oral cavity. Gene expression analysis revealed differences in the expression levels of genes from several biological pathways. Using hierarchical clustering analysis, we found that the apoptosis network was significantly altered in patients with chronic inflammation in the oral cavity, with up-regulation of pro-survival members of the Bcl-2 family and down-regulation of pro-apoptosis members in the same compartment. Additional functional analysis confirmed that the percentages of viable neutrophils are significantly increased in the oral cavity of chronic periodontitis patients.<h4>Conclusions</h4>Oral neutrophils from patients with periodontal disease displayed an altered transcriptome following migration into the oral tissues. This resulted in a pro-survival neutrophil phenotype in chronic periodontitis patients when compared with healthy subjects, resulting in a longer-lived neutrophil. This is likely to impact the severity and length of the inflammatory response in this oral disease.
format	article
author	Flavia S Lakschevitz Guy M Aboodi Michael Glogauer
author_facet	Flavia S Lakschevitz Guy M Aboodi Michael Glogauer
author_sort	Flavia S Lakschevitz
title	Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.
title_short	Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.
title_full	Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.
title_fullStr	Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.
title_full_unstemmed	Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.
title_sort	oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ce04de63426a427fa7baa5bd5a5266fb
work_keys_str_mv	AT flaviaslakschevitz oralneutrophiltranscriptomechangesresultinaprosurvivalphenotypeinperiodontaldiseases AT guymaboodi oralneutrophiltranscriptomechangesresultinaprosurvivalphenotypeinperiodontaldiseases AT michaelglogauer oralneutrophiltranscriptomechangesresultinaprosurvivalphenotypeinperiodontaldiseases
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Oral neutrophil transcriptome changes result in a pro-survival phenotype in periodontal diseases.

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