FAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.

Meiosis is a complex process involving the expression and interaction of numerous genes in a series of highly orchestrated molecular events. Fam9b localized in Xp22.3 has been found to be expressed in testes. However, FAM9B expression, localization, and its role in meiosis have not been previously r...

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Autores principales:	Xin-Jie Zhuang, Xue Feng, Wen-Hao Tang, Jin-Liang Zhu, Ming Li, Jun-Sheng Li, Xiao-Ying Zheng, Rong Li, Ping Liu, Jie Qiao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce0857eb43c94a128fee1791dc36d8a2
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spelling	oai:doaj.org-article:ce0857eb43c94a128fee1791dc36d8a22021-12-02T20:14:40ZFAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.1932-620310.1371/journal.pone.0257248https://doaj.org/article/ce0857eb43c94a128fee1791dc36d8a22021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257248https://doaj.org/toc/1932-6203Meiosis is a complex process involving the expression and interaction of numerous genes in a series of highly orchestrated molecular events. Fam9b localized in Xp22.3 has been found to be expressed in testes. However, FAM9B expression, localization, and its role in meiosis have not been previously reported. In this study, FAM9B expression was evaluated in the human testes and ovaries by RT-PCR, qPCR, and western blotting. FAM9B was found in the nuclei of primary spermatocytes in testes and specifically localized in the synaptonemal complex (SC) region of spermatocytes. FAM9B was also evident in the follicle cell nuclei and diffusely dispersed in the granular cell cytoplasm. FAM9B was partly co-localized with SYCP3, which is essential for both formation and maintenance of lateral SC elements. In addition, FAM9B had a similar distribution pattern and co-localization as γH2AX, which is a novel biomarker for DNA double-strand breaks during meiosis. All results indicate that FAM9B is a novel meiosis-associated protein that is co-localized with SYCP3 and γH2AX and may play an important role in SC formation and DNA recombination during meiosis. These findings offer a new perspective for understanding the molecular mechanisms involved in meiosis of human gametogenesis.Xin-Jie ZhuangXue FengWen-Hao TangJin-Liang ZhuMing LiJun-Sheng LiXiao-Ying ZhengRong LiPing LiuJie QiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257248 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin-Jie Zhuang Xue Feng Wen-Hao Tang Jin-Liang Zhu Ming Li Jun-Sheng Li Xiao-Ying Zheng Rong Li Ping Liu Jie Qiao FAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.
description	Meiosis is a complex process involving the expression and interaction of numerous genes in a series of highly orchestrated molecular events. Fam9b localized in Xp22.3 has been found to be expressed in testes. However, FAM9B expression, localization, and its role in meiosis have not been previously reported. In this study, FAM9B expression was evaluated in the human testes and ovaries by RT-PCR, qPCR, and western blotting. FAM9B was found in the nuclei of primary spermatocytes in testes and specifically localized in the synaptonemal complex (SC) region of spermatocytes. FAM9B was also evident in the follicle cell nuclei and diffusely dispersed in the granular cell cytoplasm. FAM9B was partly co-localized with SYCP3, which is essential for both formation and maintenance of lateral SC elements. In addition, FAM9B had a similar distribution pattern and co-localization as γH2AX, which is a novel biomarker for DNA double-strand breaks during meiosis. All results indicate that FAM9B is a novel meiosis-associated protein that is co-localized with SYCP3 and γH2AX and may play an important role in SC formation and DNA recombination during meiosis. These findings offer a new perspective for understanding the molecular mechanisms involved in meiosis of human gametogenesis.
format	article
author	Xin-Jie Zhuang Xue Feng Wen-Hao Tang Jin-Liang Zhu Ming Li Jun-Sheng Li Xiao-Ying Zheng Rong Li Ping Liu Jie Qiao
author_facet	Xin-Jie Zhuang Xue Feng Wen-Hao Tang Jin-Liang Zhu Ming Li Jun-Sheng Li Xiao-Ying Zheng Rong Li Ping Liu Jie Qiao
author_sort	Xin-Jie Zhuang
title	FAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.
title_short	FAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.
title_full	FAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.
title_fullStr	FAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.
title_full_unstemmed	FAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.
title_sort	fam9b serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ce0857eb43c94a128fee1791dc36d8a2
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FAM9B serves as a novel meiosis-related protein localized in meiotic chromosome cores and is associated with human gametogenesis.

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