FATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO

While conducting numerous studies, including researchers in our laboratory, it was found that Th1/Th2 balance plays an essential role in the regulation of reactions that determine the outcomes of immunopathological processes in both chronic and acute GVHD models. However, the question about activity...

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Autores principales:	E. N. Demchenko, E. D. Gavrilova, E. V. Goiman, N. N. Volckiy
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	systemic lupus erythematosus experimental model fatty acid ligands gpr84 th1/th2 balance Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ce10d3da3cd4471cbaf0bf2fdeaae6ca
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spelling	oai:doaj.org-article:ce10d3da3cd4471cbaf0bf2fdeaae6ca2021-11-18T08:03:51ZFATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO1563-06252313-741X10.15789/1563-0625-FAR-2235https://doaj.org/article/ce10d3da3cd4471cbaf0bf2fdeaae6ca2021-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2235https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWhile conducting numerous studies, including researchers in our laboratory, it was found that Th1/Th2 balance plays an essential role in the regulation of reactions that determine the outcomes of immunopathological processes in both chronic and acute GVHD models. However, the question about activity of which element in the regulatory process during GVHD induction (for example, a receptor or an enzyme) affects the ratio of this balance depends remains open. It has been suggested that the degree of activation of the GPR84 receptor during GVHD induction can significantly affect the host Th1/Th2 balance. And, by assessing this parameter, the direction of development and the intensity of the pathological process can be determined. The aim of this work was to investigate the effect of ligands such as medium-chain fatty acid receptor GPR84 on the Th1/Th2 balance in an experimental model in an in vivo system.Female DBA/2 and hybrids (C57Bl/6 × DBA/2) F1 mouse strains were used in the experiments.The studied ligands of GPR84 were capric and lauric acids, as well as a synthetic ligand 6-OAU. Chronic GVHD in the semi-allogenic system was induced by injecting splenocytes from DBA/2 mice to B6D2F1 hybrid mice: 60-70 × 106 -cells iv twice with an interval of 6 days. The first administration of the GPR84 ligands was performed one hour after the donor cell transfer and then once a day for two weeks.The effect of the study drugs on the course of chronic GVHD was assessed three months after the onset of the experiment.It was shown that the administration of GPR84 ligands to to animals during the induction of chronic GVHD affects the activity of the receptor and the host Th1/Th2 ratio. In the group with the injection of 6-OAU, the number of animals which the immunopathological process developed according to the Th1-dependent variant increased by more than 1.5-fold, compared with the control group. This fact is consistent with the literature data obtained in the in vitro system. Apparently, the effect of a mixture of capric and lauric acids is mediated by some other mechanism, differed from the GPR84 activation. Therefore, further research is required to realize the promising possibility of adjusting immune responses by including certain fatty acids in the diet.E. N. DemchenkoE. D. GavrilovaE. V. GoimanN. N. VolckiySPb RAACIarticlesystemic lupus erythematosusexperimental modelfatty acid ligandsgpr84th1/th2 balanceImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 4, Pp 659-664 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	systemic lupus erythematosus experimental model fatty acid ligands gpr84 th1/th2 balance Immunologic diseases. Allergy RC581-607
spellingShingle	systemic lupus erythematosus experimental model fatty acid ligands gpr84 th1/th2 balance Immunologic diseases. Allergy RC581-607 E. N. Demchenko E. D. Gavrilova E. V. Goiman N. N. Volckiy FATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO
description	While conducting numerous studies, including researchers in our laboratory, it was found that Th1/Th2 balance plays an essential role in the regulation of reactions that determine the outcomes of immunopathological processes in both chronic and acute GVHD models. However, the question about activity of which element in the regulatory process during GVHD induction (for example, a receptor or an enzyme) affects the ratio of this balance depends remains open. It has been suggested that the degree of activation of the GPR84 receptor during GVHD induction can significantly affect the host Th1/Th2 balance. And, by assessing this parameter, the direction of development and the intensity of the pathological process can be determined. The aim of this work was to investigate the effect of ligands such as medium-chain fatty acid receptor GPR84 on the Th1/Th2 balance in an experimental model in an in vivo system.Female DBA/2 and hybrids (C57Bl/6 × DBA/2) F1 mouse strains were used in the experiments.The studied ligands of GPR84 were capric and lauric acids, as well as a synthetic ligand 6-OAU. Chronic GVHD in the semi-allogenic system was induced by injecting splenocytes from DBA/2 mice to B6D2F1 hybrid mice: 60-70 × 106 -cells iv twice with an interval of 6 days. The first administration of the GPR84 ligands was performed one hour after the donor cell transfer and then once a day for two weeks.The effect of the study drugs on the course of chronic GVHD was assessed three months after the onset of the experiment.It was shown that the administration of GPR84 ligands to to animals during the induction of chronic GVHD affects the activity of the receptor and the host Th1/Th2 ratio. In the group with the injection of 6-OAU, the number of animals which the immunopathological process developed according to the Th1-dependent variant increased by more than 1.5-fold, compared with the control group. This fact is consistent with the literature data obtained in the in vitro system. Apparently, the effect of a mixture of capric and lauric acids is mediated by some other mechanism, differed from the GPR84 activation. Therefore, further research is required to realize the promising possibility of adjusting immune responses by including certain fatty acids in the diet.
format	article
author	E. N. Demchenko E. D. Gavrilova E. V. Goiman N. N. Volckiy
author_facet	E. N. Demchenko E. D. Gavrilova E. V. Goiman N. N. Volckiy
author_sort	E. N. Demchenko
title	FATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO
title_short	FATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO
title_full	FATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO
title_fullStr	FATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO
title_full_unstemmed	FATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO
title_sort	fatty acid receptor gpr84 and th1/th2 balance in the experimental system in vivo
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/ce10d3da3cd4471cbaf0bf2fdeaae6ca
work_keys_str_mv	AT endemchenko fattyacidreceptorgpr84andth1th2balanceintheexperimentalsysteminvivo AT edgavrilova fattyacidreceptorgpr84andth1th2balanceintheexperimentalsysteminvivo AT evgoiman fattyacidreceptorgpr84andth1th2balanceintheexperimentalsysteminvivo AT nnvolckiy fattyacidreceptorgpr84andth1th2balanceintheexperimentalsysteminvivo
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FATTY ACID RECEPTOR GPR84 AND Th1/Th2 BALANCE IN THE EXPERIMENTAL SYSTEM IN VIVO

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