Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.

In mammals, myocardial cell death due to infarction results in scar formation and little regenerative response. In contrast, zebrafish have a high capacity to regenerate the heart after surgical resection of myocardial tissue. However, whether zebrafish can also regenerate lesions caused by cell dea...

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Autores principales:	Kristin Schnabel, Chi-Chung Wu, Thomas Kurth, Gilbert Weidinger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce159b04ecc449498947fa9560e0d5cc
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spelling	oai:doaj.org-article:ce159b04ecc449498947fa9560e0d5cc2021-11-18T06:55:53ZRegeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.1932-620310.1371/journal.pone.0018503https://doaj.org/article/ce159b04ecc449498947fa9560e0d5cc2011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21533269/?tool=EBIhttps://doaj.org/toc/1932-6203In mammals, myocardial cell death due to infarction results in scar formation and little regenerative response. In contrast, zebrafish have a high capacity to regenerate the heart after surgical resection of myocardial tissue. However, whether zebrafish can also regenerate lesions caused by cell death has not been tested. Here, we present a simple method for induction of necrotic lesions in the adult zebrafish heart based on cryoinjury. Despite widespread tissue death and loss of cardiomyocytes caused by these lesions, zebrafish display a robust regenerative response, which results in substantial clearing of the necrotic tissue and little scar formation. The cellular mechanisms underlying regeneration appear to be similar to those activated in response to ventricular resection. In particular, the epicardium activates a developmental gene program, proliferates and covers the lesion. Concomitantly, mature uninjured cardiomyocytes become proliferative and invade the lesion. Our injury model will be a useful tool to study the molecular mechanisms of natural heart regeneration in response to necrotic cell death.Kristin SchnabelChi-Chung WuThomas KurthGilbert WeidingerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18503 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristin Schnabel Chi-Chung Wu Thomas Kurth Gilbert Weidinger Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.
description	In mammals, myocardial cell death due to infarction results in scar formation and little regenerative response. In contrast, zebrafish have a high capacity to regenerate the heart after surgical resection of myocardial tissue. However, whether zebrafish can also regenerate lesions caused by cell death has not been tested. Here, we present a simple method for induction of necrotic lesions in the adult zebrafish heart based on cryoinjury. Despite widespread tissue death and loss of cardiomyocytes caused by these lesions, zebrafish display a robust regenerative response, which results in substantial clearing of the necrotic tissue and little scar formation. The cellular mechanisms underlying regeneration appear to be similar to those activated in response to ventricular resection. In particular, the epicardium activates a developmental gene program, proliferates and covers the lesion. Concomitantly, mature uninjured cardiomyocytes become proliferative and invade the lesion. Our injury model will be a useful tool to study the molecular mechanisms of natural heart regeneration in response to necrotic cell death.
format	article
author	Kristin Schnabel Chi-Chung Wu Thomas Kurth Gilbert Weidinger
author_facet	Kristin Schnabel Chi-Chung Wu Thomas Kurth Gilbert Weidinger
author_sort	Kristin Schnabel
title	Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.
title_short	Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.
title_full	Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.
title_fullStr	Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.
title_full_unstemmed	Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.
title_sort	regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/ce159b04ecc449498947fa9560e0d5cc
work_keys_str_mv	AT kristinschnabel regenerationofcryoinjuryinducednecroticheartlesionsinzebrafishisassociatedwithepicardialactivationandcardiomyocyteproliferation AT chichungwu regenerationofcryoinjuryinducednecroticheartlesionsinzebrafishisassociatedwithepicardialactivationandcardiomyocyteproliferation AT thomaskurth regenerationofcryoinjuryinducednecroticheartlesionsinzebrafishisassociatedwithepicardialactivationandcardiomyocyteproliferation AT gilbertweidinger regenerationofcryoinjuryinducednecroticheartlesionsinzebrafishisassociatedwithepicardialactivationandcardiomyocyteproliferation
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Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation.

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