Persistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study

Longitudinal mapping of antibody-based SARS-CoV-2 immunity is critical for public health control of the pandemic and vaccine development. We performed a longitudinal analysis of the antibody-based immune response in a cohort of 100 COVID-19 individuals who were infected during the first wave of infe...

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Autores principales:	Gloria Griffante, Shikha Chandel, Daniela Ferrante, Valeria Caneparo, Daniela Capello, Valentina Bettio, Cinzia Borgogna, Chiara Aleni, Salvatore Esposito, Andrea Sarro, Alessandra Vasile, Marco Comba, Tommaso Testa, Gianmarco Cotrupi, Marco De Andrea, Sara Bortoluzzi, Marisa Gariglio
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 COVID-19 neutralizing humoral response Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ce1eef91640446459a231ab1612a69cd
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spelling	oai:doaj.org-article:ce1eef91640446459a231ab1612a69cd2021-11-25T19:13:58ZPersistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study10.3390/v131122701999-4915https://doaj.org/article/ce1eef91640446459a231ab1612a69cd2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2270https://doaj.org/toc/1999-4915Longitudinal mapping of antibody-based SARS-CoV-2 immunity is critical for public health control of the pandemic and vaccine development. We performed a longitudinal analysis of the antibody-based immune response in a cohort of 100 COVID-19 individuals who were infected during the first wave of infection in northern Italy. The SARS-CoV-2 humoral response was tested using the COVID-SeroIndex, Kantaro Quantitative SARS-CoV-2 IgG Antibody RUO Kit (R&D Systems, Bio-Techne, Minneapolis, USA) and pseudotype-based neutralizing antibody assay. Using sequential serum samples collected from 100 COVID-19 recovered individuals from northern Italy—mostly with mild disease—at 2 and 10 months after their first positive PCR test, we show that 93% of them seroconverted at 2 months, with a geometric mean (GeoMean) half-maximal neutralization titer (NT50) of 387.9. Among the 35 unvaccinated subjects retested at 10 months, 7 resulted seronegative, with an 80% drop in seropositivity, while 28 showed decreased anti-receptor binding domain (RBD) and anti-spike (S) IgG titers, with a GeoMean NT50 neutralization titer dropping to 163.5. As an NT50 > 100 is known to confer protection from SARS-CoV-2 re-infection, our data show that the neutralizing activity elicited by the natural infection has lasted for at least 10 months in a large fraction of subjects.Gloria GriffanteShikha ChandelDaniela FerranteValeria CaneparoDaniela CapelloValentina BettioCinzia BorgognaChiara AleniSalvatore EspositoAndrea SarroAlessandra VasileMarco CombaTommaso TestaGianmarco CotrupiMarco De AndreaSara BortoluzziMarisa GariglioMDPI AGarticleSARS-CoV-2COVID-19neutralizing humoral responseMicrobiologyQR1-502ENViruses, Vol 13, Iss 2270, p 2270 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 COVID-19 neutralizing humoral response Microbiology QR1-502
spellingShingle	SARS-CoV-2 COVID-19 neutralizing humoral response Microbiology QR1-502 Gloria Griffante Shikha Chandel Daniela Ferrante Valeria Caneparo Daniela Capello Valentina Bettio Cinzia Borgogna Chiara Aleni Salvatore Esposito Andrea Sarro Alessandra Vasile Marco Comba Tommaso Testa Gianmarco Cotrupi Marco De Andrea Sara Bortoluzzi Marisa Gariglio Persistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study
description	Longitudinal mapping of antibody-based SARS-CoV-2 immunity is critical for public health control of the pandemic and vaccine development. We performed a longitudinal analysis of the antibody-based immune response in a cohort of 100 COVID-19 individuals who were infected during the first wave of infection in northern Italy. The SARS-CoV-2 humoral response was tested using the COVID-SeroIndex, Kantaro Quantitative SARS-CoV-2 IgG Antibody RUO Kit (R&D Systems, Bio-Techne, Minneapolis, USA) and pseudotype-based neutralizing antibody assay. Using sequential serum samples collected from 100 COVID-19 recovered individuals from northern Italy—mostly with mild disease—at 2 and 10 months after their first positive PCR test, we show that 93% of them seroconverted at 2 months, with a geometric mean (GeoMean) half-maximal neutralization titer (NT50) of 387.9. Among the 35 unvaccinated subjects retested at 10 months, 7 resulted seronegative, with an 80% drop in seropositivity, while 28 showed decreased anti-receptor binding domain (RBD) and anti-spike (S) IgG titers, with a GeoMean NT50 neutralization titer dropping to 163.5. As an NT50 > 100 is known to confer protection from SARS-CoV-2 re-infection, our data show that the neutralizing activity elicited by the natural infection has lasted for at least 10 months in a large fraction of subjects.
format	article
author	Gloria Griffante Shikha Chandel Daniela Ferrante Valeria Caneparo Daniela Capello Valentina Bettio Cinzia Borgogna Chiara Aleni Salvatore Esposito Andrea Sarro Alessandra Vasile Marco Comba Tommaso Testa Gianmarco Cotrupi Marco De Andrea Sara Bortoluzzi Marisa Gariglio
author_facet	Gloria Griffante Shikha Chandel Daniela Ferrante Valeria Caneparo Daniela Capello Valentina Bettio Cinzia Borgogna Chiara Aleni Salvatore Esposito Andrea Sarro Alessandra Vasile Marco Comba Tommaso Testa Gianmarco Cotrupi Marco De Andrea Sara Bortoluzzi Marisa Gariglio
author_sort	Gloria Griffante
title	Persistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study
title_short	Persistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study
title_full	Persistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study
title_fullStr	Persistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study
title_full_unstemmed	Persistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study
title_sort	persistence of neutralizing antibodies to sars-cov-2 in first wave infected individuals at ten months post-infection: the unirsa cohort study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ce1eef91640446459a231ab1612a69cd
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Persistence of Neutralizing Antibodies to SARS-CoV-2 in First Wave Infected Individuals at Ten Months Post-Infection: The UnIRSA Cohort Study

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