Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice

Ruonan Bo,1 Yaqin Sun,1 Shuzhen Zhou,2 Ning Ou,1 Pengfei Gu,1 Zhenguang Liu,1 Yuanliang Hu,1 Jiaguo Liu,1 Deyun Wang1 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2Foshan City Nanhai Eastern Along Pharmaceutical Co....

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Autores principales:	Bo R, Sun Y, Zhou S, Ou N, Gu P, Liu Z, Hu Y, Liu J, Wang D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Lycium barbarum polysaccharide liposome Adjuvant Ovalbumin Draining lymph nodes Antigen-specific humoral response Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ce224c61b7ae4b429a03130da6e6969b
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spelling	oai:doaj.org-article:ce224c61b7ae4b429a03130da6e6969b2021-12-02T02:04:23ZSimple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice1178-2013https://doaj.org/article/ce224c61b7ae4b429a03130da6e6969b2017-08-01T00:00:00Zhttps://www.dovepress.com/simple-nanoliposomes-encapsulating-lycium-barbarum-polysaccharides-as--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ruonan Bo,1 Yaqin Sun,1 Shuzhen Zhou,2 Ning Ou,1 Pengfei Gu,1 Zhenguang Liu,1 Yuanliang Hu,1 Jiaguo Liu,1 Deyun Wang1 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2Foshan City Nanhai Eastern Along Pharmaceutical Co., Ltd, Foshan, China Abstract: The success of subunit vaccines has been hampered by the problems of weak or short-term immunity and the lack of availability of nontoxic, potent adjuvants. It would be desirable to develop safe and efficient adjuvants with the aim of improving the cellular immune response against the target antigen. In this study, the targeting and sustained release of simple nanoliposomes containing Lycium barbarum polysaccharides (LBP) as an efficacious immune adjuvant to improve immune responses were explored. LBP liposome (LBPL) with high entrapment efficiency (86%) were obtained using a reverse-phase evaporation method and then used to encapsulate the model antigen, ovalbumin (OVA). We demonstrated that the as-synthesized liposome loaded with OVA and LBP (LBPL-OVA) was stable for 45 days and determined the encapsulation stability of OVA at 4°C and 37°C and the release profile of OVA from LBPL-OVA was investigated in pH 7.4 and pH 5.0. Further in vivo investigation showed that the antigen-specific humoral response was correlated with antigen delivery to the draining lymph nodes. The LBPL-OVA were also associated with high levels of uptake by key dendritic cells in the draining lymph nodes and they efficiently stimulated CD4+ and CD8+ T cell proliferation in vivo, further promoting antibody production. These features together elicited a significant humoral and celluar immune response, which was superior to that produced by free antigen alone. Keywords: Lycium barbarum polysaccharide liposome, adjuvant, ovalbumin, draining lymph nodes, antigen-specific humoral responseBo RSun YZhou SOu NGu PLiu ZHu YLiu JWang DDove Medical PressarticleLycium barbarum polysaccharide liposomeAdjuvantOvalbuminDraining lymph nodesAntigen-specific humoral responseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6289-6301 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lycium barbarum polysaccharide liposome Adjuvant Ovalbumin Draining lymph nodes Antigen-specific humoral response Medicine (General) R5-920
spellingShingle	Lycium barbarum polysaccharide liposome Adjuvant Ovalbumin Draining lymph nodes Antigen-specific humoral response Medicine (General) R5-920 Bo R Sun Y Zhou S Ou N Gu P Liu Z Hu Y Liu J Wang D Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice
description	Ruonan Bo,1 Yaqin Sun,1 Shuzhen Zhou,2 Ning Ou,1 Pengfei Gu,1 Zhenguang Liu,1 Yuanliang Hu,1 Jiaguo Liu,1 Deyun Wang1 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2Foshan City Nanhai Eastern Along Pharmaceutical Co., Ltd, Foshan, China Abstract: The success of subunit vaccines has been hampered by the problems of weak or short-term immunity and the lack of availability of nontoxic, potent adjuvants. It would be desirable to develop safe and efficient adjuvants with the aim of improving the cellular immune response against the target antigen. In this study, the targeting and sustained release of simple nanoliposomes containing Lycium barbarum polysaccharides (LBP) as an efficacious immune adjuvant to improve immune responses were explored. LBP liposome (LBPL) with high entrapment efficiency (86%) were obtained using a reverse-phase evaporation method and then used to encapsulate the model antigen, ovalbumin (OVA). We demonstrated that the as-synthesized liposome loaded with OVA and LBP (LBPL-OVA) was stable for 45 days and determined the encapsulation stability of OVA at 4°C and 37°C and the release profile of OVA from LBPL-OVA was investigated in pH 7.4 and pH 5.0. Further in vivo investigation showed that the antigen-specific humoral response was correlated with antigen delivery to the draining lymph nodes. The LBPL-OVA were also associated with high levels of uptake by key dendritic cells in the draining lymph nodes and they efficiently stimulated CD4+ and CD8+ T cell proliferation in vivo, further promoting antibody production. These features together elicited a significant humoral and celluar immune response, which was superior to that produced by free antigen alone. Keywords: Lycium barbarum polysaccharide liposome, adjuvant, ovalbumin, draining lymph nodes, antigen-specific humoral response
format	article
author	Bo R Sun Y Zhou S Ou N Gu P Liu Z Hu Y Liu J Wang D
author_facet	Bo R Sun Y Zhou S Ou N Gu P Liu Z Hu Y Liu J Wang D
author_sort	Bo R
title	Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice
title_short	Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice
title_full	Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice
title_fullStr	Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice
title_full_unstemmed	Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice
title_sort	simple nanoliposomes encapsulating lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/ce224c61b7ae4b429a03130da6e6969b
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Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice

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