Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China

ABSTRACT In 2017, a new type of goose-origin astrovirus (GoAstV) that is completely different from previously identified avian astroviruses (which have only 30.0% to 50.5% homology with GoAstV) has been isolated from diseased geese in China. This disease can cause joint swelling in sick geese, and t...

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Autores principales:	Xiaoyuan Yuan, Kai Meng, Yuxia Zhang, Lihong Qi, Wu Ai, Youling Wang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	goose-origin astrovirus RT-qPCR TaqMan probe Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ce265fb7a2b04f4eb3dff68f98d63bd2
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spelling	oai:doaj.org-article:ce265fb7a2b04f4eb3dff68f98d63bd22021-11-15T15:22:21ZEstablishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China10.1128/mSphere.00380-182379-5042https://doaj.org/article/ce265fb7a2b04f4eb3dff68f98d63bd22018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00380-18https://doaj.org/toc/2379-5042ABSTRACT In 2017, a new type of goose-origin astrovirus (GoAstV) that is completely different from previously identified avian astroviruses (which have only 30.0% to 50.5% homology with GoAstV) has been isolated from diseased geese in China. This disease can cause joint swelling in sick geese, and the anatomy shows a clear precipitation of urate in the kidney. The rate of death and culling can reach more than 30%, revealing the disease’s severe pathogenicity. To quickly and accurately diagnose the newly emerging disease, we established a highly specific reverse transcription-quantitative PCR (RT-qPCR) method of detecting GoAstV. Sensitivity testing showed that the minimum amount of test sample for this method is 52.5 copies/μl. Clinical application confirmed that this method can quickly and effectively detect GoAstV, providing a diagnostic platform for the prevention and control of goose disease. IMPORTANCE Goose-origin astrovirus (GoAstV), as a newly emerging virus in 2017, is different from previously known astroviruses in the genus Avastrovirus. So far, few studies have focused on the novel virus. Considering the infectious development of astrovirus (AstV), we established a reverse transcription-quantitative PCR (RT-qPCR) assay with a strong specificity to quickly and accurately diagnose GoAstV. Confirmed by clinical application, this method can quickly and accurately detect prevalent GoAstV. The assay is thus convenient for clinical operation and is applicable to the monitoring of GoAstV disease.Xiaoyuan YuanKai MengYuxia ZhangLihong QiWu AiYouling WangAmerican Society for Microbiologyarticlegoose-origin astrovirusRT-qPCRTaqMan probeMicrobiologyQR1-502ENmSphere, Vol 3, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	goose-origin astrovirus RT-qPCR TaqMan probe Microbiology QR1-502
spellingShingle	goose-origin astrovirus RT-qPCR TaqMan probe Microbiology QR1-502 Xiaoyuan Yuan Kai Meng Yuxia Zhang Lihong Qi Wu Ai Youling Wang Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
description	ABSTRACT In 2017, a new type of goose-origin astrovirus (GoAstV) that is completely different from previously identified avian astroviruses (which have only 30.0% to 50.5% homology with GoAstV) has been isolated from diseased geese in China. This disease can cause joint swelling in sick geese, and the anatomy shows a clear precipitation of urate in the kidney. The rate of death and culling can reach more than 30%, revealing the disease’s severe pathogenicity. To quickly and accurately diagnose the newly emerging disease, we established a highly specific reverse transcription-quantitative PCR (RT-qPCR) method of detecting GoAstV. Sensitivity testing showed that the minimum amount of test sample for this method is 52.5 copies/μl. Clinical application confirmed that this method can quickly and effectively detect GoAstV, providing a diagnostic platform for the prevention and control of goose disease. IMPORTANCE Goose-origin astrovirus (GoAstV), as a newly emerging virus in 2017, is different from previously known astroviruses in the genus Avastrovirus. So far, few studies have focused on the novel virus. Considering the infectious development of astrovirus (AstV), we established a reverse transcription-quantitative PCR (RT-qPCR) assay with a strong specificity to quickly and accurately diagnose GoAstV. Confirmed by clinical application, this method can quickly and accurately detect prevalent GoAstV. The assay is thus convenient for clinical operation and is applicable to the monitoring of GoAstV disease.
format	article
author	Xiaoyuan Yuan Kai Meng Yuxia Zhang Lihong Qi Wu Ai Youling Wang
author_facet	Xiaoyuan Yuan Kai Meng Yuxia Zhang Lihong Qi Wu Ai Youling Wang
author_sort	Xiaoyuan Yuan
title	Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
title_short	Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
title_full	Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
title_fullStr	Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
title_full_unstemmed	Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
title_sort	establishment and application of rapid diagnosis for reverse transcription-quantitative pcr of newly emerging goose-origin nephrotic astrovirus in china
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/ce265fb7a2b04f4eb3dff68f98d63bd2
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Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China

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