Comparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility

ABSTRACT Plant root-associated microbes promote plant growth and elicit induced systemic resistance (ISR) to foliar pathogens. In an attempt to find novel growth-promoting and ISR-inducing strains, we previously identified strains of root-associated Pseudomonas spp. that promote plant growth but une...

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Autores principales:	Polina Beskrovnaya, Ryan A. Melnyk, Zhexian Liu, Yang Liu, Melanie A. Higgins, Yi Song, Katherine S. Ryan, Cara H. Haney
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	rhizosphere microbiome induced systemic susceptibility Pseudomonas Arabidopsis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ce2d410e3d984061a0b959e71895d258
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spelling	oai:doaj.org-article:ce2d410e3d984061a0b959e71895d2582021-11-15T15:56:46ZComparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility10.1128/mBio.00575-202150-7511https://doaj.org/article/ce2d410e3d984061a0b959e71895d2582020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00575-20https://doaj.org/toc/2150-7511ABSTRACT Plant root-associated microbes promote plant growth and elicit induced systemic resistance (ISR) to foliar pathogens. In an attempt to find novel growth-promoting and ISR-inducing strains, we previously identified strains of root-associated Pseudomonas spp. that promote plant growth but unexpectedly elicited induced systemic susceptibility (ISS) rather than ISR to foliar pathogens. Here, we demonstrate that the ISS-inducing phenotype is common among root-associated Pseudomonas spp. Using comparative genomics, we identified a single Pseudomonas fluorescens locus that is unique to ISS strains. We generated a clean deletion of the 11-gene ISS locus and found that it is necessary for the ISS phenotype. Although the functions of the predicted genes in the locus are not apparent based on similarity to genes of known function, the ISS locus is present in diverse bacteria, and a subset of the genes were previously implicated in pathogenesis in animals. Collectively, these data show that a single bacterial locus contributes to modulation of systemic plant immunity. IMPORTANCE Microbiome-associated bacteria can have diverse effects on health of their hosts, yet the genetic and molecular bases of these effects have largely remained elusive. This work demonstrates that a novel bacterial locus can modulate systemic plant immunity. Additionally, this work demonstrates that growth-promoting strains may have unanticipated consequences for plant immunity, and this is critical to consider when the plant microbiome is being engineered for agronomic improvement.Polina BeskrovnayaRyan A. MelnykZhexian LiuYang LiuMelanie A. HigginsYi SongKatherine S. RyanCara H. HaneyAmerican Society for Microbiologyarticlerhizospheremicrobiomeinduced systemic susceptibilityPseudomonasArabidopsisMicrobiologyQR1-502ENmBio, Vol 11, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	rhizosphere microbiome induced systemic susceptibility Pseudomonas Arabidopsis Microbiology QR1-502
spellingShingle	rhizosphere microbiome induced systemic susceptibility Pseudomonas Arabidopsis Microbiology QR1-502 Polina Beskrovnaya Ryan A. Melnyk Zhexian Liu Yang Liu Melanie A. Higgins Yi Song Katherine S. Ryan Cara H. Haney Comparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility
description	ABSTRACT Plant root-associated microbes promote plant growth and elicit induced systemic resistance (ISR) to foliar pathogens. In an attempt to find novel growth-promoting and ISR-inducing strains, we previously identified strains of root-associated Pseudomonas spp. that promote plant growth but unexpectedly elicited induced systemic susceptibility (ISS) rather than ISR to foliar pathogens. Here, we demonstrate that the ISS-inducing phenotype is common among root-associated Pseudomonas spp. Using comparative genomics, we identified a single Pseudomonas fluorescens locus that is unique to ISS strains. We generated a clean deletion of the 11-gene ISS locus and found that it is necessary for the ISS phenotype. Although the functions of the predicted genes in the locus are not apparent based on similarity to genes of known function, the ISS locus is present in diverse bacteria, and a subset of the genes were previously implicated in pathogenesis in animals. Collectively, these data show that a single bacterial locus contributes to modulation of systemic plant immunity. IMPORTANCE Microbiome-associated bacteria can have diverse effects on health of their hosts, yet the genetic and molecular bases of these effects have largely remained elusive. This work demonstrates that a novel bacterial locus can modulate systemic plant immunity. Additionally, this work demonstrates that growth-promoting strains may have unanticipated consequences for plant immunity, and this is critical to consider when the plant microbiome is being engineered for agronomic improvement.
format	article
author	Polina Beskrovnaya Ryan A. Melnyk Zhexian Liu Yang Liu Melanie A. Higgins Yi Song Katherine S. Ryan Cara H. Haney
author_facet	Polina Beskrovnaya Ryan A. Melnyk Zhexian Liu Yang Liu Melanie A. Higgins Yi Song Katherine S. Ryan Cara H. Haney
author_sort	Polina Beskrovnaya
title	Comparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility
title_short	Comparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility
title_full	Comparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility
title_fullStr	Comparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility
title_full_unstemmed	Comparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility
title_sort	comparative genomics identified a genetic locus in plant-associated <italic toggle="yes">pseudomonas</italic> spp. that is necessary for induced systemic susceptibility
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/ce2d410e3d984061a0b959e71895d258
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Comparative Genomics Identified a Genetic Locus in Plant-Associated <italic toggle="yes">Pseudomonas</italic> spp. That Is Necessary for Induced Systemic Susceptibility

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