Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry

Abstract Antimicrobial resistance against colistin has emerged worldwide and is threatening the efficacy of colistin treatment of multi-resistant Gram-negative bacteria. In this study, PCRs were used to detect mcr genes (mcr-1, mcr-2, mcr-3) in 213 anal and 1,339 nasal swabs from pigs (n = 1,454) in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jilei Zhang, Li Chen, Jiawei Wang, Afrah Kamal Yassin, Patrick Butaye, Patrick Kelly, Jiansen Gong, Weina Guo, Jing Li, Min Li, Feng Yang, Zhixing Feng, Ping Jiang, Chunlian Song, Yaoyao Wang, Jinfeng You, Yi Yang, Stuart Price, Kezong Qi, Yuan Kang, Chengming Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce368cae87d147faa6f75fe41c8c4f5e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ce368cae87d147faa6f75fe41c8c4f5e
record_format	dspace
spelling	oai:doaj.org-article:ce368cae87d147faa6f75fe41c8c4f5e2021-12-02T15:08:51ZMolecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry10.1038/s41598-018-22084-42045-2322https://doaj.org/article/ce368cae87d147faa6f75fe41c8c4f5e2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-22084-4https://doaj.org/toc/2045-2322Abstract Antimicrobial resistance against colistin has emerged worldwide and is threatening the efficacy of colistin treatment of multi-resistant Gram-negative bacteria. In this study, PCRs were used to detect mcr genes (mcr-1, mcr-2, mcr-3) in 213 anal and 1,339 nasal swabs from pigs (n = 1,454) in nine provinces of China, and 1,696 cloacal and 1,647 oropharyngeal samples from poultry (n = 1,836) at live-bird markets in 24 provinces. The mcr-1 prevalences in pigs (79.2%) and geese (71.7%) were significantly higher than in chickens (31.8%), ducks (34.6%) and pigeons (13.1%). The mcr-2 prevalence in pigs was 56.3%, significantly higher than in chickens (5.5%), ducks (2.3%), geese (5.5%) and pigeons (0%). The mcr-3 prevalences in pigs (18.7%), ducks (13.8%) and geese (11.9%) were significantly higher than in chickens (5.2%) and pigeons (5.1%). In total, 173 pigs and three chickens were positive for all three mcr genes. The prevalences of the mcr were significantly higher in nasal/oropharyngeal swabs than in the anal /cloacal swabs. Phylogenetic studies identified 33 new mcr-2 variants and 12 new mcr-3 variants. This study demonstrates high prevalences of mcr in pigs and poultry in China, and indicates there is need for more thorough surveillance and control programs to prevent further selection of colistin resistance.Jilei ZhangLi ChenJiawei WangAfrah Kamal YassinPatrick ButayePatrick KellyJiansen GongWeina GuoJing LiMin LiFeng YangZhixing FengPing JiangChunlian SongYaoyao WangJinfeng YouYi YangStuart PriceKezong QiYuan KangChengming WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jilei Zhang Li Chen Jiawei Wang Afrah Kamal Yassin Patrick Butaye Patrick Kelly Jiansen Gong Weina Guo Jing Li Min Li Feng Yang Zhixing Feng Ping Jiang Chunlian Song Yaoyao Wang Jinfeng You Yi Yang Stuart Price Kezong Qi Yuan Kang Chengming Wang Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry
description	Abstract Antimicrobial resistance against colistin has emerged worldwide and is threatening the efficacy of colistin treatment of multi-resistant Gram-negative bacteria. In this study, PCRs were used to detect mcr genes (mcr-1, mcr-2, mcr-3) in 213 anal and 1,339 nasal swabs from pigs (n = 1,454) in nine provinces of China, and 1,696 cloacal and 1,647 oropharyngeal samples from poultry (n = 1,836) at live-bird markets in 24 provinces. The mcr-1 prevalences in pigs (79.2%) and geese (71.7%) were significantly higher than in chickens (31.8%), ducks (34.6%) and pigeons (13.1%). The mcr-2 prevalence in pigs was 56.3%, significantly higher than in chickens (5.5%), ducks (2.3%), geese (5.5%) and pigeons (0%). The mcr-3 prevalences in pigs (18.7%), ducks (13.8%) and geese (11.9%) were significantly higher than in chickens (5.2%) and pigeons (5.1%). In total, 173 pigs and three chickens were positive for all three mcr genes. The prevalences of the mcr were significantly higher in nasal/oropharyngeal swabs than in the anal /cloacal swabs. Phylogenetic studies identified 33 new mcr-2 variants and 12 new mcr-3 variants. This study demonstrates high prevalences of mcr in pigs and poultry in China, and indicates there is need for more thorough surveillance and control programs to prevent further selection of colistin resistance.
format	article
author	Jilei Zhang Li Chen Jiawei Wang Afrah Kamal Yassin Patrick Butaye Patrick Kelly Jiansen Gong Weina Guo Jing Li Min Li Feng Yang Zhixing Feng Ping Jiang Chunlian Song Yaoyao Wang Jinfeng You Yi Yang Stuart Price Kezong Qi Yuan Kang Chengming Wang
author_facet	Jilei Zhang Li Chen Jiawei Wang Afrah Kamal Yassin Patrick Butaye Patrick Kelly Jiansen Gong Weina Guo Jing Li Min Li Feng Yang Zhixing Feng Ping Jiang Chunlian Song Yaoyao Wang Jinfeng You Yi Yang Stuart Price Kezong Qi Yuan Kang Chengming Wang
author_sort	Jilei Zhang
title	Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry
title_short	Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry
title_full	Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry
title_fullStr	Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry
title_full_unstemmed	Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry
title_sort	molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ce368cae87d147faa6f75fe41c8c4f5e
work_keys_str_mv	AT jileizhang moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT lichen moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT jiaweiwang moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT afrahkamalyassin moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT patrickbutaye moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT patrickkelly moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT jiansengong moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT weinaguo moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT jingli moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT minli moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT fengyang moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT zhixingfeng moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT pingjiang moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT chunliansong moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT yaoyaowang moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT jinfengyou moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT yiyang moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT stuartprice moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT kezongqi moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT yuankang moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry AT chengmingwang moleculardetectionofcolistinresistancegenesmcr1mcr2andmcr3innasaloropharyngealandanalcloacalswabsfrompigsandpoultry
_version_	1718388028423012352

Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry

Ejemplares similares