In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.

Bifidobacteria have received significant attention due to their contribution to human gut health and the use of specific strains as probiotics. It is thus not surprising that there has also been significant interest with respect to their antibiotic resistance profile. Numerous culture-based studies...

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Autores principales:	Fiona Fouhy, Mary O'Connell Motherway, Gerald F Fitzgerald, R Paul Ross, Catherine Stanton, Douwe van Sinderen, Paul D Cotter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce369888ac964c3ea57b631da9f2d55b
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spelling	oai:doaj.org-article:ce369888ac964c3ea57b631da9f2d55b2021-11-18T08:43:02ZIn silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.1932-620310.1371/journal.pone.0082653https://doaj.org/article/ce369888ac964c3ea57b631da9f2d55b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24324818/?tool=EBIhttps://doaj.org/toc/1932-6203Bifidobacteria have received significant attention due to their contribution to human gut health and the use of specific strains as probiotics. It is thus not surprising that there has also been significant interest with respect to their antibiotic resistance profile. Numerous culture-based studies have demonstrated that bifidobacteria are resistant to the majority of aminoglycosides, but are sensitive to β-lactams. However, limited research exists with respect to the genetic basis for the resistance of bifidobacteria to aminoglycosides. Here we performed an in-depth in silico analysis of putative Bifidobacterium-encoded aminoglycoside resistance proteins and β-lactamases and assess the contribution of these proteins to antibiotic resistance. The in silico-based screen detected putative aminoglycoside and β-lactam resistance proteins across the Bifidobacterium genus. Laboratory-based investigations of a number of representative bifidobacteria strains confirmed that despite containing putative β-lactamases, these strains were sensitive to β-lactams. In contrast, all strains were resistant to the aminoglycosides tested. To assess the contribution of genes encoding putative aminoglycoside resistance proteins in Bifidobacterium sp. two genes, namely Bbr_0651 and Bbr_1586, were targeted for insertional inactivation in B. breve UCC2003. As compared to the wild-type, the UCC2003 insertion mutant strains exhibited decreased resistance to gentamycin, kanamycin and streptomycin. This study highlights the associated risks of relying on the in silico assignment of gene function. Although several putative β-lactam resistance proteins are located in bifidobacteria, their presence does not coincide with resistance to these antibiotics. In contrast however, this approach has resulted in the identification of two loci that contribute to the aminoglycoside resistance of B. breve UCC2003 and, potentially, many other bifidobacteria.Fiona FouhyMary O'Connell MotherwayGerald F FitzgeraldR Paul RossCatherine StantonDouwe van SinderenPaul D CotterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82653 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fiona Fouhy Mary O'Connell Motherway Gerald F Fitzgerald R Paul Ross Catherine Stanton Douwe van Sinderen Paul D Cotter In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.
description	Bifidobacteria have received significant attention due to their contribution to human gut health and the use of specific strains as probiotics. It is thus not surprising that there has also been significant interest with respect to their antibiotic resistance profile. Numerous culture-based studies have demonstrated that bifidobacteria are resistant to the majority of aminoglycosides, but are sensitive to β-lactams. However, limited research exists with respect to the genetic basis for the resistance of bifidobacteria to aminoglycosides. Here we performed an in-depth in silico analysis of putative Bifidobacterium-encoded aminoglycoside resistance proteins and β-lactamases and assess the contribution of these proteins to antibiotic resistance. The in silico-based screen detected putative aminoglycoside and β-lactam resistance proteins across the Bifidobacterium genus. Laboratory-based investigations of a number of representative bifidobacteria strains confirmed that despite containing putative β-lactamases, these strains were sensitive to β-lactams. In contrast, all strains were resistant to the aminoglycosides tested. To assess the contribution of genes encoding putative aminoglycoside resistance proteins in Bifidobacterium sp. two genes, namely Bbr_0651 and Bbr_1586, were targeted for insertional inactivation in B. breve UCC2003. As compared to the wild-type, the UCC2003 insertion mutant strains exhibited decreased resistance to gentamycin, kanamycin and streptomycin. This study highlights the associated risks of relying on the in silico assignment of gene function. Although several putative β-lactam resistance proteins are located in bifidobacteria, their presence does not coincide with resistance to these antibiotics. In contrast however, this approach has resulted in the identification of two loci that contribute to the aminoglycoside resistance of B. breve UCC2003 and, potentially, many other bifidobacteria.
format	article
author	Fiona Fouhy Mary O'Connell Motherway Gerald F Fitzgerald R Paul Ross Catherine Stanton Douwe van Sinderen Paul D Cotter
author_facet	Fiona Fouhy Mary O'Connell Motherway Gerald F Fitzgerald R Paul Ross Catherine Stanton Douwe van Sinderen Paul D Cotter
author_sort	Fiona Fouhy
title	In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.
title_short	In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.
title_full	In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.
title_fullStr	In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.
title_full_unstemmed	In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.
title_sort	in silico assigned resistance genes confer bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ce369888ac964c3ea57b631da9f2d55b
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In silico assigned resistance genes confer Bifidobacterium with partial resistance to aminoglycosides but not to β-lactams.

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