Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)

Barley production is essential in Egypt. In the present study, 15 different six-rowed Egyptian barley cultivars were studied. To differentiate between the different cultivars under study in terms of morphological characteristics and ISSR, molecular characterization reactions were carried out. Moreov...

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Autores principales:	Azza H. Mohamed, Ahmad A. Omar, Ahmed M. Attya, Mohamed M. A. Elashtokhy, Ehab M. Zayed, Rehab M. Rizk
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plastid markers DNA barcoding ISSR markers Egyptian barley agro-morphological traits cluster analysis Botany QK1-989
Acceso en línea:	https://doaj.org/article/ce3a6ce1c1f040368da9a9f94dc8d343
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spelling	oai:doaj.org-article:ce3a6ce1c1f040368da9a9f94dc8d3432021-11-25T18:47:25ZMorphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)10.3390/plants101125272223-7747https://doaj.org/article/ce3a6ce1c1f040368da9a9f94dc8d3432021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2527https://doaj.org/toc/2223-7747Barley production is essential in Egypt. In the present study, 15 different six-rowed Egyptian barley cultivars were studied. To differentiate between the different cultivars under study in terms of morphological characteristics and ISSR, molecular characterization reactions were carried out. Moreover, four cultivars (Giza 123, Giza 126, Giza 136, and Giza 138) were selected for further studies using scanning electron microscopy (SEM). Computational analysis of the DNA barcoding sequences of the two plastid markers <i>rbc</i>L and <i>mat</i>K was executed, and the results were deposited in the NCBI database. The morphological traits showed low statistical significance among the different cultivars under study via the data collected from two seasons, suggesting that the mean field performance of these Egyptian cultivars may be equal under these conditions. The results showed that the phylogenetic tree was divided into four groups, one of which contained the most closely related genotypes in the genetic distance, including Giza 124, Giza 130, Giza 138, Giza 136, and Giza 137, which converge in the indicative uses of farmers. The seed coat of the studied cultivars was “rugose”. The elevation folding of the rugose pattern ranged from 11 ± 1.73 µm (Giza 126) to 14.67 ± 2.43 µm (Giza 123), suggesting variation in seed quality and its uses in feed and the food industry. According to the similarity matrix of ISSR analysis, the highest similarity value (93%) was recorded between Giza 133 and Giza 132, as well as between Giza 2000 and Giza 126. On the other hand, the lowest similarity value (80%) was recorded between Giza 130 and (Giza 133 and Giza 132), indicating that these cultivars were distantly related. Polymorphism information content (PIC) ranged from 0.26 for the primer ISSR UBC 835 to 0.37 for the primers ISSR UBC 814 and ISSR UBC 840. The current study showed that the <i>mat</i>K gene is more mutable than the <i>rbc</i>L gene among the tested cultivars.Azza H. MohamedAhmad A. OmarAhmed M. AttyaMohamed M. A. ElashtokhyEhab M. ZayedRehab M. RizkMDPI AGarticleplastid markersDNA barcodingISSR markersEgyptian barleyagro-morphological traitscluster analysisBotanyQK1-989ENPlants, Vol 10, Iss 2527, p 2527 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plastid markers DNA barcoding ISSR markers Egyptian barley agro-morphological traits cluster analysis Botany QK1-989
spellingShingle	plastid markers DNA barcoding ISSR markers Egyptian barley agro-morphological traits cluster analysis Botany QK1-989 Azza H. Mohamed Ahmad A. Omar Ahmed M. Attya Mohamed M. A. Elashtokhy Ehab M. Zayed Rehab M. Rizk Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)
description	Barley production is essential in Egypt. In the present study, 15 different six-rowed Egyptian barley cultivars were studied. To differentiate between the different cultivars under study in terms of morphological characteristics and ISSR, molecular characterization reactions were carried out. Moreover, four cultivars (Giza 123, Giza 126, Giza 136, and Giza 138) were selected for further studies using scanning electron microscopy (SEM). Computational analysis of the DNA barcoding sequences of the two plastid markers <i>rbc</i>L and <i>mat</i>K was executed, and the results were deposited in the NCBI database. The morphological traits showed low statistical significance among the different cultivars under study via the data collected from two seasons, suggesting that the mean field performance of these Egyptian cultivars may be equal under these conditions. The results showed that the phylogenetic tree was divided into four groups, one of which contained the most closely related genotypes in the genetic distance, including Giza 124, Giza 130, Giza 138, Giza 136, and Giza 137, which converge in the indicative uses of farmers. The seed coat of the studied cultivars was “rugose”. The elevation folding of the rugose pattern ranged from 11 ± 1.73 µm (Giza 126) to 14.67 ± 2.43 µm (Giza 123), suggesting variation in seed quality and its uses in feed and the food industry. According to the similarity matrix of ISSR analysis, the highest similarity value (93%) was recorded between Giza 133 and Giza 132, as well as between Giza 2000 and Giza 126. On the other hand, the lowest similarity value (80%) was recorded between Giza 130 and (Giza 133 and Giza 132), indicating that these cultivars were distantly related. Polymorphism information content (PIC) ranged from 0.26 for the primer ISSR UBC 835 to 0.37 for the primers ISSR UBC 814 and ISSR UBC 840. The current study showed that the <i>mat</i>K gene is more mutable than the <i>rbc</i>L gene among the tested cultivars.
format	article
author	Azza H. Mohamed Ahmad A. Omar Ahmed M. Attya Mohamed M. A. Elashtokhy Ehab M. Zayed Rehab M. Rizk
author_facet	Azza H. Mohamed Ahmad A. Omar Ahmed M. Attya Mohamed M. A. Elashtokhy Ehab M. Zayed Rehab M. Rizk
author_sort	Azza H. Mohamed
title	Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)
title_short	Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)
title_full	Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)
title_fullStr	Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)
title_full_unstemmed	Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)
title_sort	morphological and molecular characterization of some egyptian six-rowed barley (<i>hordeum vulgare</i> l.)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ce3a6ce1c1f040368da9a9f94dc8d343
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Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (<i>Hordeum vulgare</i> L.)

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